A5020120616- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Antifungal resistance and susceptibility
- Immune Cell Function and Interaction
- Infectious Diseases and Tuberculosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Fungal Infections and Studies
- Antibiotics Pharmacokinetics and Efficacy
- Diagnosis and treatment of tuberculosis
- IL-33, ST2, and ILC Pathways
- Immune cells in cancer
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Immunodeficiency and Autoimmune Disorders
- Helminth infection and control
- COVID-19 Clinical Research Studies
- Drug Transport and Resistance Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Biosensors and Analytical Detection
- Pneumonia and Respiratory Infections
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nematode management and characterization studies
- Ocular Infections and Treatments
- Antibiotic Resistance in Bacteria
Shenzhen Third People’s Hospital
2016-2025
Southern University of Science and Technology
2020-2025
National Clinical Research
2023-2024
Second Affiliated Hospital of Xi'an Jiaotong University
2021-2024
First Affiliated Hospital of Xi'an Jiaotong University
2015-2024
Shanghai Clinical Research Center
2023-2024
Xi'an Jiaotong University
2015-2024
Xi'an Medical University
2024
Shanghai Ocean University
2023
Shenyang Jianzhu University
2020-2023
ABSTRACT Background The outbreak of novel coronavirus pneumonia (NCP) caused by 2019-nCoV spread rapidly, and elucidating the diagnostic accuracy different respiratory specimens is crucial for control treatment this disease. Methods Respiratory samples including nasal swabs, throat sputum bronchoalveolar lavage fluid (BALF) were collected from Guangdong CDC confirmed NCP patients, viral RNAs detected using a CFDA approved detection kit. Results analyzed in combination with sample collection...
T cell immunoglobulin-3 (TIM-3) has been established as a negative regulatory molecule and plays critical role in immune tolerance. TIM-3 is upregulated exhausted CD8(+) cells both chronic infection tumor. However, the nature of TIM-3(+)CD4(+) tumor microenvironment unclear. This study to characterize expressing lymphocytes within human lung cancer tissues establish clinical significance expression progression.A total 51 tissue specimens were obtained from pathologically confirmed newly...
The worldwide epidemic of coronavirus disease 2019 (COVID-19) is ongoing. Rapid and accurate detection the causative virus SARS-CoV-2 vital for treatment control COVID-19. In this study, comparative sensitivity different respiratory specimen types were retrospectively analyzed using 3,552 clinical samples from 410 COVID-19 patients confirmed by Guangdong CDC (Center Disease Control Prevention). Except bronchoalveolar lavage fluid (BALF), sputum possessed highest positive rate (73.4%-87.5%),...
Abstract Understanding the mechanism that leads to immune dysfunction in severe coronavirus disease 2019 (COVID-19) is crucial for development of effective treatment. Here, using single-cell RNA sequencing, we characterized peripheral blood mononuclear cells (PBMCs) from uninfected controls and COVID-19 patients paired broncho-alveolar lavage fluid (BALF). We found a close association decreased dendritic (DCs) increased monocytes resembling myeloid-derived suppressor (MDSCs), which...
Cancer immunotherapy has shown great promise as a new standard cancer therapeutic modality. However, the response rates are limited for current approach that depends on enhancing spontaneous antitumor immune responses. Therefore, increasing tumor immunogenicity by expressing appropriate cytokines should further improve immunotherapy. IL-33 is member of IL-1 family and released necrotic epithelial cells or activated innate thus considered "danger" signal. The role in promoting type 2...
Abstract The effector functions of CD8 + T cells are influenced by tissue inflammatory microenvironments. IL‐33, a member the IL‐1 family, acts as danger signal after its release during cell necrosis. IL‐33/ST2 axis has been implicated in various Th2 responses. Its role T‐cell‐mediated immune response is, however, not known. Here we find that type 1 cytotoxic (Tc1) cultured vitro unexpectedly express high levels IL‐33 receptor ST2. Interestingly, expression ST2 Tc1 is dependent on T‐bet,...
Although it is well recognized that CD4(+) T cells and helper (Th) 1 cytokines are critical in the cell-mediated response to Mycobacterium tuberculosis, also clear this immunity alone not enough. Understanding roles of other cell subsets essential for vaccine design clinical immunotherapy against tuberculosis (TB).To investigate significance possible regulatory mechanism Th17 responses human TB.The frequencies IFN-gamma-, IL-4-, IL-17-, FoxP3- IL-6 receptor (IL-6R)-expressing blood and/or...
We developed a chemiluminescence immunoassay method based on the recombinant nucleocapsid antigen and assessed its performance for clinical diagnosis of severe acute respiratory syndrome coronavirus (SARS-CoV)-2 infections by detecting SARS-CoV-2–specific IgM IgG antibodies in patients. Full-length tosyl magnetic beads were used to develop approach. Plasmas from 29 healthy cohorts, 51 tuberculosis patients, 79 confirmed SARS-CoV-2 patients employed evaluate infections. A commercial ELISA kit...
Complement functions as an important host defense system and complement C5 C7 have been implicated in immunopathology of tuberculosis. However, little is known about the role other components tuberculosis.Complement gene expression peripheral blood mononuclear cells tuberculosis patients controls were determined using whole genome transcriptional microarray assays. The mRNA protein levels three C1q components, C1qA, C1qB, C1qC, further validated by qRT-PCR enzyme-linked immunosorbent assay,...
T cells are strongly regulated by oxidizing environments and amino acid restriction. How reprogram metabolism to adapt these extracellular stress situations is not well understood. Here, we show that starvation induce ATF4 in CD4
Mycobacterium tuberculosis (Mtb) infection, including active (TB) and latent Mtb infection (LTBI), leads to diverse outcomes owing different host immune responses. However, the mechanisms that govern progression from LTBI TB remain poorly defined in humans. Here, we profiled lung cell populations within bronchoalveolar lavage fluid (BALF) patients with or using single-cell RNA sequencing (scRNA-seq). We found substantially changed compartments BALF, especially for three subsets of...
ABSTRACT Mice deficient for granulocyte-macrophage colony-stimulating factor (GM-CSF −/− ) are highly susceptible to infection with Mycobacterium tuberculosis , and clinical data have shown that anti-GM-CSF neutralizing antibodies can lead increased susceptibility in otherwise healthy people. GM-CSF activates human murine macrophages inhibit intracellular M. growth. We previously produced by iNKT cells inhibits growth of . However, the more general role T cell-derived during has not been...
Orchestration of an effective T lymphocyte response at infection sites is critical for protection against Mycobacterium tuberculosis (Mtb) infection. However, the local cell immunity landscape in human poorly defined. Tuberculous pleural effusion (TPE), caused by Mtb, characterized influx leukocytes to space, providing a platform suitable delineating complex tissue responses Mtb Using single-cell transcriptomics and receptor sequencing, we analyzed mononuclear populations paired fluid...
Glycosylation, a fundamental biological process, involves the attachment of glycans to proteins, lipids, and RNA, it plays crucial role in various pathways. It is great significance obtain precise spatial distribution glycosylation modifications at cellular tissue levels. Here, we introduce LectoScape, an innovative method enabling detailed imaging glycomes with up 1 μm resolution through image mass cytometry (IMC). This utilizes 12 distinct, nonoverlapping lectins selected via microarray...
Memory T cells are composed of effector, central, and memory stem cells. Previous studies have implicated that both T-bet Eomes involved in the generation effector central CD8 The exact role these transcription factors shaping cell pool is not well understood, particularly with Here, we demonstrate or required for elimination established tumors by adoptively transferred We also examined tumor-specific subsets upon adoptive transfer. showed combined deficiency resulted a severe reduction...
We sought to describe the population pharmacokinetics of tigecycline in critically ill patients and determine optimized dosing regimens for different bacterial infections. This prospective study included 10 given a standard dose tigecycline. Blood samples were collected during one interval analyzed using validated chromatography. Population Monte Carlo simulations undertaken Pmetrics. Three target exposures, expressed as ratios 24-h area under curve MICs (AUC0-24/MIC), evaluated (≥17.9 skin...
Abstract The ongoing SARS-CoV-2 outbreak has killed over twenty-one thousand and sickened four hundred people worldwide, posing a great challenge to global public health. A sensitive accurate diagnosis method will substantially help control disease expansion. Here, we developed chemiluminescence-immunoassay based on the recombinant nucleocapsid antigen magnetic beads for of infections surveillance antibody changing pattern. Serums from 29 healthy individuals, 51 tuberculosis patients, 79...
Abstract Tissue-resident memory T cells (TRMs) have a key role in mediating the host defense against tuberculosis (TB) mice, but their human counterparts not been well characterized. In this article, we recruited patients with TB and determined TRM frequency, trafficking, activation marker expression, cytokine production by flow or mass cytometry at different infection sites, including peripheral blood, pleural fluid, bronchoalveolar lavage lung. We found high frequency of TRMs all sites...