A5003177235- Pituitary Gland Disorders and Treatments
- Neuroendocrine Tumor Research Advances
- Biochemical Analysis and Sensing Techniques
- Regulation of Appetite and Obesity
- melanin and skin pigmentation
- Receptor Mechanisms and Signaling
- Chemical Synthesis and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Glioma Diagnosis and Treatment
- Neuropeptides and Animal Physiology
- Protein purification and stability
- Cyclopropane Reaction Mechanisms
- Folate and B Vitamins Research
- Advanced Biosensing Techniques and Applications
- Glycosylation and Glycoproteins Research
- TGF-β signaling in diseases
- Sleep and Wakefulness Research
- Growth Hormone and Insulin-like Growth Factors
- Chemokine receptors and signaling
- Adipokines, Inflammation, and Metabolic Diseases
- Cutaneous Melanoma Detection and Management
- Advanced Proteomics Techniques and Applications
- Peptidase Inhibition and Analysis
- Phenothiazines and Benzothiazines Synthesis and Activities
- BRCA gene mutations in cancer
Latvian Biomedical Research and Study Centre
2013-2024
University of Latvia
1997-2021
Uppsala University
2000-2008
Summary The expression of melanocortin MC1 receptors on human peripheral lymphocyte subsets was analysed by flow cytometry using rabbit antibodies selective for the receptor and a panel monoclonal against differentiation markers. found to be constitutively expressed monocytes/macrophages, B-lymphocytes, natural killer (NK) cells subset cytotoxic T-cells. Interestingly T-helper appeared essentially devoid receptors. results were confirmed RT-PCR which indicated strong mRNA in CD14+, CD19+...
Polyherbal formulations Jathyadi Thailam and Jatyadi Ghritam (JT) are used in Indian traditional medicine for diabetic chronic wounds, fistula, fissure, eczema, burn management. We aimed to investigate the antibacterial anti-inflammatory properties of crude hexane ethanol extracts JT formulations.Antibacterial activity was tested estimate minimum inhibitory concentrations (MICs) against nine reference bacterial strains, including one methicillin-resistant Staphylococcus aureus (MRSA)...
Proteochemometrics is a technology for the study of molecular recognition based on chemometric techniques. Here we applied it to analyse amino acids and acid physico-chemical properties that are involved in antibodies' peptide antigens. To this end, used system comprised by diverse single chain antibody library derived from murine mAb anti-p24 (HIV-1) CB4-1, evaluated arrays manufactured SPOT synthesis. The binding pattern obtained was correlated descriptors (z-scales) antibodies peptides...
Members of the hydroxycarboxylic acid receptor (HCA1-3) family are mainly expressed in adipocytes and immune cells. HCA2 ligand, niacin, has been used for decades as lipid-modifying drug. Recent studies suggest that HCA ligands can be involved modulation inflammatory processes. In this study, we evaluated effects HCA1-3 on adipose differentiation cytokine expression human macrophages. Simpson-Golabi-Behmel syndrome (SGBS) preadipocytes were induced to differentiate into 8 d presence or...
Pituitary adenomas are one of the most common endocrine and intracranial neoplasms. Although they theoretically monoclonal in origin, several studies have shown that contain different multipotent cell types thought to play an important role tumor initiation, maintenance, recurrence after therapy. In present study, we isolated characterized populations from seven pituitary somatotroph, nonhormonal, lactotroph adenomas. The obtained cells showed characteristics mesenchymal stromal as observed...
Abstract Pituitary neuroendocrine tumours (PitNETs) are neoplasms of the pituitary that overproduce hormones or cause unspecific symptoms due to mass effect. Growth hormone overproducing GH-producing PitNETs acromegaly leading connective tissue, metabolic oncologic disorders. The medical treatment is somatostatin analogues (SSA) in specific cases combined with dopamine agonists (DA), but almost half patients display partial full SSA resistance and potential causes this unknown. In study we...
Abstract Tunicates are evolutionary model organisms bridging the gap between vertebrates and invertebrates. A genomic sequence in Ciona intestinalis (CiOX) shows high similarity to vertebrate orexin receptors protostome allatotropin (ATR). Here, molecular phylogeny suggested that CiOX is divergent from ATRs human (hOX 1/2 ). However, appears closer hOX than ATR both terms of percent identity its modelled binding cavity, as by modelling. was heterologously expressed a recombinant HEK293 cell...
The most common type of pituitary neoplasms is benign adenoma (PA). Clinically significant PAs affect around 0.1% the population. Currently, there no established human PA cell culture available and when tumour cells are cultured they form two distinct types depending on culturing conditions either free-floating aggregates also known as pituispheres or adhering to surface plates displaying mesenchymal stem-like properties. aim this study was trace origin sphere-forming adherent cultures...
Acromegaly is a disease mainly caused by pituitary neuroendocrine tumor (PitNET) overproducing growth hormone. First-line medication for this condition the use of somatostatin analogs (SSAs), that decrease mass and induce antiproliferative effects on PitNET cells. Dopamine agonists (DAs) can also be used if SSA treatment not effective. This study aimed to determine differences in transcriptome signatures induced SSA/DA therapy tissue. We selected tissue from twelve patients with...
Abstract The aim of this study was to develop predictive quantitative structure–activity relationship (QSAR) modeling for antibody‐peptide interactions. A small single chain antibody library designed and manufactured around the murine anti‐p24 (HIV‐1) monoclonal CB4‐1 by use statistical molecular design (SMD) principles site directed mutagenesis, its affinity a p24 derived antigen determined fluorescence polarization. satisfactory QSAR model ( Q 2 = 0.74, R 0.88) correlating data...
The paradigm of ligand-receptor interactions postulated as "one compound-one target" has been evolving; a multi-target, pleiotropic approach is now considered to be realistic. Novel series 1,4,5,6,7,8-hexahydro-5-oxoquinolines, pyranopyrimidines and S-alkyl derivatives have synthesized in order characterise their pleiotropic, multitarget activity on the FFA3/GPR41, FFA2/GPR43, HCA2/GPR109A receptors. Hexahydroquinoline known exhibit characteristic FFA3/GPR41 ligands, but during this study we...
In the title compound, C 25 H NO 5 S, which exhibits metabolism-regulating activity, 1,4-dihydropyridine ring adopts a flattened boat conformation while cyclohexenone is in an envelope conformation. Molecules crystal are assembled into (6) chains along -axis direction via N—H...O hydrogen bonds. The thienyl fragment disordered over two sets of sites 0.7220 (19):0.2780 (19) ratio.
Abstract We demonstrate the use of statistical molecular design (SMD) in selection peptide libraries aimed to systematically investigate antigen‐antibody binding spaces. Earlier, we derived two novel antibodies by mutating complementarity‐determining region anti‐p24 (HIV‐1) single chain Fv antibody, CB4‐1 that had lost their affinity for a p24 epitope‐homologous 8‐ and 60‐fold. The present study was devoted explore how can be designed under experimental criteria effective screening antigens....