A5103027143- Air Quality and Health Impacts
- Indoor Air Quality and Microbial Exposure
- Atmospheric chemistry and aerosols
- Energy and Environment Impacts
- Climate Change and Health Impacts
- RNA Research and Splicing
- RNA regulation and disease
- DNA Repair Mechanisms
- Genetics and Neurodevelopmental Disorders
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- interferon and immune responses
- Air Quality Monitoring and Forecasting
- Natural Fiber Reinforced Composites
- Orthopaedic implants and arthroplasty
- Particle Dynamics in Fluid Flows
- Cellular Mechanics and Interactions
- Inhalation and Respiratory Drug Delivery
- Chromosomal and Genetic Variations
- Chemokine receptors and signaling
- Virology and Viral Diseases
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
Helmholtz Zentrum München
2020-2024
Stockholm University
2019-2022
Paul Ehrlich Institut
2010-2015
Novel (Germany)
2011
Background: Secondary organic aerosols (SOAs) formed from anthropogenic or biogenic gaseous precursors in the atmosphere substantially contribute to ambient fine particulate matter [PM ≤2.5μm aerodynamic diameter (PM2.5)] burden, which has been associated with adverse human health effects. However, there is only limited evidence on their differential toxicological impact. Objectives: We aimed discriminate effects of generated by atmospheric aging combustion soot particles (SPs) (β-pinene)...
ABSTRACT In 2012, the first cases of infection with Middle East respiratory syndrome coronavirus (MERS-CoV) were identified. Since then, more than 1,000 MERS-CoV have been confirmed; is typically associated considerable morbidity and, in approximately 30% cases, mortality. Currently, there no protective vaccine available. Replication-competent recombinant measles virus (MV) expressing foreign antigens constitutes a promising tool to induce immunity against corresponding pathogens. Therefore,...
Abstract Background Wood combustion emissions have been studied previously either by in vitro or vivo models using collected particles, yet most studies neglected gaseous compounds. Furthermore, a more accurate and holistic view of the toxicity aerosols can be gained with parallel direct exposure methods. Moreover, modern techniques such as air-liquid interface (ALI) exposures enable better assessment applied than, for example, previous state-of-the-art submerged cell techniques. Methods We...
The health effects of exposure to secondary organic aerosols (SOAs) are still limited. Here, we investigated and compared the toxicities soot particles (SP) coated with β-pinene SOA (SOAβPin-SP) SP naphthalene (SOANap-SP) in a human bronchial epithelial cell line (BEAS-2B) residing at air–liquid interface. SOAβPin-SP mostly contained oxygenated aliphatic compounds from photooxidation, whereas SOANap-SP significant fraction aromatic products under similar conditions. Following exposure,...
Interfering with interferon: A low-molecular-weight inhibitor has been discovered that blocks the interaction between interferon-α (IFN-α) and its receptor (see picture for a model of interfaces). The resulting lead compound significantly reduces IFN-α production in vitro. NMR SPR experiments confirm direct IFN-α.
Soot particles (SP) are ubiquitous components of atmospheric particulate matter and have been shown to cause various adverse health effects. In the atmosphere, freshly emitted SP can be coated by condensed low-volatility secondary organic inorganic species. addition, gas-phase oxidants may react with surface SP. Due chemical physical resemblance carbon backbone polyaromatic hydrocarbon species their potent oxidation products, we investigated biological responses BEAS-2B lung epithelial cells...
Solid fuel usage in residential heating and cooking is one of the largest sources ambient indoor air particulate matter, which causes adverse effects on health millions peoples worldwide. Emissions from solid combustion, such as biomass or coal, are detrimental to health, but toxicological responses largely unknown. In present study, we compared regarding cytotoxicity, inflammation genotoxicity spruce (SPR) brown coal briquette (BCB) combustion aerosols human alveolar epithelial cells (A549)...
Type I interferon (IFN-α/β) induction upon viral infection contributes to the early antiviral host defense and ensures survival until onset of adaptive immunity. Many infections lead an acute, transient IFN expression which peaks a few hours after reverts initial levels 24 36 h. Robust often is conferred by specialized plasmacytoid dendritic cells (pDC) may depend on positive-feedback amplification via type receptor (IFNAR). Here, we show that mice infected with Thogoto virus (THOV),...
We present the development and application of a new machine-learning approach to exhaustively reliably identify major histocompatibility complex class I (MHC-I) ligands among all 208 octapeptides in genome-derived proteomes Mus musculus, influenza A H3N8, vesicular stomatitis virus (VSV). Focusing on murine H-2Kb, we identified potent exhibiting direct MHC-I binding stabilization surface TAP-deficient RMA-S cells. Computationally VSV-derived peptides induced CD8+ T-cell proliferation after...
Resolution of branched DNA structures is pivotal for repair stalled replication forks and meiotic recombination intermediates. The Yen1 nuclease cleaves both Holliday junctions forks. We show that interacts physically with Uls1, a suggested SUMO-targeted ubiquitin ligase also contains SWI/SNF-family ATPase-domain. SUMO-modified in its noncatalytic carboxyl terminus damage induces SUMOylation. SUMO-modification strengthens the interaction to mutations SUMO motifs Uls1 weakens interaction....
Cell exposure experiments at the air-liquid interface (ALI) are used increasingly as indicators for health effects and impact of aerosols on lung. Thereby aerosol particles kept airborne can deposit a cell surface area similar to human respiratory tract (RT). However, geometry air flow rates an ALI system deviate considerably from RT. As tissue-delivered particle dose lungs (TD) hardly be measured, computer models deposition here mimic both in An setup (VitroCell GmbH) airflow rate 100 cm3...
Abstract The most commonly used construction material nowadays is steel-reinforced concrete which underlies corrosion and thus buildings are susceptible for structural collapses. Recently, a new resistant to oxidation with higher tensile strength called carbon composite (C 3 ) was developed. allows resource-saving constructions using fiber instead of steel reinforcement materials embedded in matrix. C reinforcements consist fibers coated an organic polymer In this study, abrasive dust from...
Telomere chromatin structure is pivotal for maintaining genome stability by regulating the binding of telomere-associated proteins and inhibiting DNA damage response. In Saccharomyces cerevisiae, silent information regulator (Sir) bind to terminal repeats subtelomeric X-elements, resulting in transcriptional silencing. Herein, we show that sir2 mutant strains display a specific loss nucleosome residing X-elements this deficiency remarkably consistent between different telomeres. The contain...
Abstract The prevalence of allergic diseases is constantly increasing since few decades. Anthropogenic ultrafine particles (UFPs) and allergenic aerosols highly involved in this increase; however, the underlying cellular mechanisms are not yet understood. Studies observing these effects focused mainly on singular vivo or vitro exposures single particle sources, while there only limited evidence their subsequent combined effects. Our study aimed at evaluating effect to allergy‐related...