A5023659596- Ovarian cancer diagnosis and treatment
- Cytokine Signaling Pathways and Interactions
- Lymphoma Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- Antibiotic Resistance in Bacteria
- Peptidase Inhibition and Analysis
- Cancer Mechanisms and Therapy
- MicroRNA in disease regulation
- Pharmaceutical and Antibiotic Environmental Impacts
- Cancer-related molecular mechanisms research
- Immunotherapy and Immune Responses
- Adipose Tissue and Metabolism
- RNA modifications and cancer
- Circular RNAs in diseases
- Peroxisome Proliferator-Activated Receptors
- Advanced Proteomics Techniques and Applications
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Bone Metabolism and Diseases
- Mycobacterium research and diagnosis
- Enterobacteriaceae and Cronobacter Research
- Synthesis and biological activity
- Bone health and treatments
- Helicobacter pylori-related gastroenterology studies
- Metabolism, Diabetes, and Cancer
Ningbo University Affiliated Hospital
2025
Qilu Hospital of Shandong University
2009-2024
Ningbo University
2023-2024
Liaoning Shihua University
2023
Shandong University
2008-2022
Lishui University
2018-2022
Zhejiang University
2018-2022
Gansu Agricultural University
2021
Lishui Central Hospital
2018-2020
Wenzhou Medical University
2015-2019
Protein methylation is a common posttranslational modification that mostly occurs on arginine and lysine residues. Arginine has been reported to regulate RNA processing, gene transcription, DNA damage repair, protein translocation, signal transduction. Lysine best known histone function involved in epigenetic regulation of transcription. To better study methylation, we have developed highly specific antibodies against monomethyl arginine; asymmetric dimethyl monomethyl, dimethyl, trimethyl...
Cholangiocarcinoma, also known as bile duct cancer, is the second most common primary hepatic carcinoma with a median survival of less than 2 years. The molecular mechanisms underlying development this disease are not clear. To survey activated tyrosine kinases signaling in cholangiocarcinoma, we employed immunoaffinity profiling coupled to mass spectrometry and identified DDR1, EPHA2, EGFR, ROS kinases, along over 1,000 phosphorylation sites from about 750 different proteins...
Ovarian cancer is the leading cause of death from gynecologic cancer. Improvement in clinical outcome patients likely to be achieved by identification molecular events that underlie oncogenesis ovarian Here we show anaplastic lymphoma kinase (ALK) aberrantly activated Using an unbiased and global phosphoproteomic approach, profiled 69 Chinese primary tumor tissues found ALK expressed phosphorylated 4 tumors. Genetic characterization these ALK-positive tumors indicated full-length expression...
Hypervirulent variants of Klebsiella pneumoniae (hvKp) that cause invasive community-acquired pyogenic liver abscess (PLA) have emerged globally. Little is known about the virulence determinants associated with hvKp, except for genes rmpA/A2 and siderophores (iroBCD/iucABCD) carried by pK2044-like large plasmid. Here, we collected most recent clinical isolates hvKp from PLA samples in China, performed clinical, molecular, genomic sequencing analyses. We found 90.9% (40/44) pathogens causing...
Glucagon-like peptide 1 (GLP-1), a gut-derived peptide, has been reported to have profound effects on metabolism and reduce insulin resistance. Adipocyte hyperplasia stimulated by preadipocyte differentiation positive effect adipose tissue sensitivity. However, it remains less clear whether GLP-1 plays role in adipogenesis. In this study, we examined the of investigated mechanisms that may be involved effect. our 3T3-L1 cell tested levels adipocyte-specific markers signaling pathways during...
Cognitive dysfunction often occurs in diabetes mellitus patients. This study aimed to investigate the efficacy of melatonin (MLT) improving diabetes-associated cognitive decline and underlying mechanism involved. Type 2 diabetic mice palmitic acid (PA)-stimulated BV-2 cells were treated by MLT, potential mechanisms among cognition, autophagy explored. The results showed that type obvious learning memory impairments Morris water maze test compared with normal controls, which could be...
Mantle cell lymphoma (MCL) is currently an incurable entity, and new therapeutic approaches are needed. We have applied a high-throughput phospho-proteomic technique to MCL lines identify activated pathways we then validated our data in both tumor tissues.PhosphoScan analysis was performed on lines. Results were by flow cytometry western blotting. Functional validation blocking the most active pathway lines.PhosphoScan identified more than 300 tyrosine-phosporylated proteins, among which...
<title>Abstract</title> Background COPD is a respiratory disease with significant inflammatory characteristics. Low-dose aspirin widely used as an anti-inflammatory medicine. However, the impact of low-dose on unclear. This article aims to investigate association between and prevalence COPD. Methods A cohort study was conducted based United States population data from National Health Nutrition Examination Survey (NHANES) (2011–2012, 2013–2014, 2015–2016, 2017–2020, 2021–2023). We examined...
Activating mutations of FMS-like tyrosine kinase-3 (FLT3) are found in approximately 30% patients with acute myeloid leukemia (AML). FLT3 is therefore an attractive drug target. However, the molecular mechanisms by which lead to cell transformation AML remain unclear. To develop a better understanding signaling as well its downstream effectors, we performed detailed phosphoproteomic analysis human cells. We identified over 1000 phosphorylation sites from about 750 proteins both (wild type...
MiR-155-5p is a key oncogenic microRNA that maintains immune homeostasis and mediates cross-talk between inflammation tumorigenesis. High expression of programmed death ligand-1 (PD-L1) also plays an important role in tolerance tumors. The present study aimed to explore the relationship miR-155-5p PD-L1 lung adenocarcinoma (LUAD) cells A549 H1650. levels LUAD patients were detected by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) mimics used model increased or H1650...
Fungal keratitis is an inflammation of the cornea that results from infection by fungal organisms. It has a high rate blindness, which makes accurate diagnosis important. Confocal microscopy optical imaging technique assists doctors in diagnosing keratitis, and images obtained confocal can be used to detect hyphae. The current challenges are how classify normal with nerves abnormal hyphae complicated background. To address this problem, paper proposes novel automatic detection method making...
Overweight or obesity in post-menopausal women is known to induce metabolic disorders associated with neuroinflammation. α-lipoic acid (α-LA), which has been clinically used treat diabetic peripheral neuropathy, found exert anti-inflammatory and neuroprotective effects patients Alzheimer's disease (AD). In this study, investigate the of α-LA on neuroinflammatory factors levels hippocampal brain-derived neurotrophic factor (BDNF) ovariectomized rats fed a high-fat diet (HFD), Wistar were HFD...
There is a general agreement in the brain computer interface community that feature extracting method called common spatial pattern (CSP) combined with nonlinear classifiers can provide excellent results some cases. However, CSP also known to be very sensitive noise and prone over fitting, performance of this filter closely related operational frequency band electroencephalogram data. To address issue, we propose new FB-TRCSP+RF based on random forest. The FB-TRCSP by 8th-order Butterworth...
The occurrence of non-contiguous, multiple, and remote involvement tuberculous spondylitis is rare. clinical presentation in patients with multifocal musculoskeletal tuberculosis may closely mimic that multiple bone metastases, which makes the accurate diagnosis challenging. Herein, we report a case was misdiagnosed for 8 months as metastases. A 63-year-old male farmer Chinese Han ethnicity presented to us pain left side neck, right chest back 10 without typical symptoms. His past medical...
// Jing Wu 1,* , Xue-hua Chen 2,* Xin-qiong Wang 1 Yi Yu Jian-min Ren Yuan Xiao Tong Zhou Pu Li 2 Chun-di Xu Department of Pediatrics, Ruijin Hospital, Shanghai Jiao University School Medicine, Shanghai, People’s Republic China Key Laboratory Gastric Neoplasms, Institute Digestive Surgery, * These authors have contributed equally to this work Correspondence to: Xu, email: Li, Keywords : ERp19, gastric cancer, tumorigenicity, FAK, paxillin Received January 07, 2015 Accepted March 02,...
TSH/TSHR signaling plays a role in the regulation of lipid metabolism adipocytes. However, precise mechanisms are not known. In present study, we determined effect TSH on fatty acid synthase (FASN) expression, and explored underlying mechanisms. vitro, reduced FASN expression both mRNA protein levels mature adipocytes was accompanied by kinase A (PKA) activation, cAMP‐response element binding (CREB) phosphorylation, as well extracellular signal‐regulated 1/2 (ERK1/2) c‐Jun NH 2 ‐terminal...
The plasmid-mediated transmission of antibiotic resistance genes has been reported to be involved in the development bacteria, and poses a serious threat for success bacterial infection treatment human health worldwide. present study used 454 GS‑FLX pyrosequencing system determine ~140 kb nucleotide sequence plasmid pHS36‑NDM, which was identified Salmonella Stanley isolate from stool sample an 11‑month‑old girl at Lishui Central Hospital, China, contains New Delhi metallo‑β‑lactamase‑1...
The wide spread of Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae is great threat to public health in China. Plasmids are among the major factors mediating blaKPC gene dissemination. A total 156 carbapenem-resistant (CRE) isolates were identified a tertiary hospital Six KPC-producing isolates, namely, E. coli (n = 2), asburiae 1), C. freundii portucalensis and koseri tested positive for pCKPC18-1-like untypeable plasmid, which was described recently freundii. All 6...
The expression of the blaKPC gene plays a key role in carbapenem resistance Enterobacteriaceae However, genetic regulators have not been completely elucidated, especially genes Tn3-Tn4401 chimeras. Two novel chimera isoforms were characterized our hospital, isoform A (CTA), which harbors 121-bp deletion containing PX promoter and was present 22.6% (54/239) isolates, C (CTC), 624-bp insertion P1 only 1 isolate. MICs both 2-fold or more higher than those wild type (Tn3-Tn4401 chimera, CTB),...
To investigate the molecular characteristics of carbapenem-resistant Enterobacteriaceae (CRE) from county hospitals in China.Forty-three sequential non-duplicate CRE strains (including 33 Klebsiella pneumoniae isolates, 4 Enterobacter cloacae 3 Escherichia coli 1 Serratia marcescens, Morganella morganii and Citrobacter freundii) were collected 2 municipal hospitals. Antimicrobial susceptibility testing was conducted by broth microdilution method, using 3-aminophenylboronic acid EDTA modified...