A5023366526- Acute Myeloid Leukemia Research
- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Barrier Structure and Function Studies
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
- Force Microscopy Techniques and Applications
- Cancer Genomics and Diagnostics
- Ocular Oncology and Treatments
- Photoreceptor and optogenetics research
- Acute Kidney Injury Research
- Neurogenesis and neuroplasticity mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Health Systems, Economic Evaluations, Quality of Life
- Glioma Diagnosis and Treatment
- Advanced biosensing and bioanalysis techniques
- Pharmacogenetics and Drug Metabolism
- Genetics and Neurodevelopmental Disorders
- Genetic factors in colorectal cancer
- Bacterial Identification and Susceptibility Testing
- Neutropenia and Cancer Infections
- Multiple and Secondary Primary Cancers
Stanford Health Care
2024
Vanderbilt University Medical Center
2023-2024
Boston Children's Hospital
2018-2023
Harvard University
2023
Vanderbilt University
2023
Boston University
2018
Seattle University
2011
Forebrain precursor cells are dynamic during early brain development, yet the underlying molecular changes remain elusive. We observed major differences in transcriptional signatures of from mouse forebrain at embryonic days E8.5 vs. E10.5 (before after neural tube closure). Genes encoding protein biosynthetic machinery were strongly downregulated E10.5. This was matched by decreases ribosome biogenesis and synthesis, together with age-related proteomic content adjacent fluids. Notably,...
Transmission and secretion of signals via the choroid plexus (ChP) brain barrier can modulate states regulation cerebrospinal fluid (CSF) composition. Here, we developed a platform to analyze diurnal variations in male mouse ChP CSF. Ribosome profiling epithelial cells revealed translatome differences metabolic machinery, secreted proteins, components. Using CSF metabolomics blood-CSF analyses, observed changes metabolites cellular junctions. We then focused on transthyretin (TTR), diurnally...
Massive, coordinated cellular changes accompany the transition of central nervous system (CNS) progenitors from forebrain neurectodermal cells to specified neuroepithelial cells. We previously found that c-MYC regulates changing ribosomal and proteostatic landscapes in mouse precursors at embryonic days E8.5 vs. E10.5 (before after neural tube closure; NTC) (Chau et al., 2018). Here we demonstrate parallel transcriptional metabolic machinery during this same stage specification. Progenitors...
Clonal hematopoiesis (CH) is an age-associated phenomenon leading to increased risk of both hematologic malignancy and nonmalignant organ dysfunction. Increasingly available genetic testing has made the incidental discovery CH clinically common yet evidence-based guidelines effective management strategies prevent adverse health outcomes are lacking. To address this gap, prospective CHIVE (clonal inflammation in vasculature) registry biorepository was created identify monitor individuals at...
Febrile neutropenia (FN) in pediatric patients with cancer can cause severe infections, and prompt antibiotics are warranted. Extrapolated from other populations, a time-to-antibiotic (TTA) metric of <60 minutes after medical center presentation was established, compliance data factoring into oncology program national rankings.
Despite complex pathways of drug disposition, clinical pharmacogenetic predictors currently rely on only a few high effect variants. Quantification the polygenic contribution to variability in disposition is necessary prioritize target drugs for pharmacogenomic approaches and guide analytic methods. Dexmedetomidine fentanyl, often used postoperative care pediatric patients, have rates inter-individual dosing requirements. Analyzing previously generated population pharmacokinetic parameters,...
Atomic force microscopy (AFM) allows for the visualizing of individual proteins, DNA molecules, protein-protein complexes, and DNA-protein complexes. On end microscope's cantilever is a nano-scale probe, which traverses image areas ranging from nanometers to micrometers, measuring elevation macromolecules resting on substrate surface at any given point. Electrostatic forces cause lipids, nucleic acids loosely attach in random orientations permit imaging. The generated data resemble...
The choroid plexus (ChP) epithelium is a source of secreted signaling factors in cerebrospinal fluid (CSF) and key barrier between blood brain. Here, we develop imaging tools to interrogate these functions adult lateral ventricle ChP wholemount explants awake mice. By epithelial cells intact explants, observed calcium activity secretory events that increased frequency following delivery serotonergic agonists. Using chronic two-photon mice, spontaneous subcellular as well strong...
Atomic force microscopy (AFM) allows for the visualizing of individual proteins, DNA molecules, protein-protein complexes, and DNA-protein complexes. On end microscope's cantilever is a nano-scale probe, which traverses image areas ranging from nanometers to micrometers, measuring elevation macromolecules resting on substrate surface at any given point. Electrostatic forces cause lipids, nucleic acids loosely attach in random orientations permit imaging. The generated data resemble...