A5091248796- Erythrocyte Function and Pathophysiology
- Hemoglobinopathies and Related Disorders
- Blood groups and transfusion
- Blood transfusion and management
- Neonatal Health and Biochemistry
- Blood properties and coagulation
- Erythropoietin and Anemia Treatment
- Hemoglobin structure and function
- Extracellular vesicles in disease
- Heme Oxygenase-1 and Carbon Monoxide
- Iron Metabolism and Disorders
- Blood donation and transfusion practices
- MicroRNA in disease regulation
- Dialysis and Renal Disease Management
- Methemoglobinemia and Tumor Lysis Syndrome
- Invertebrate Immune Response Mechanisms
- Circular RNAs in diseases
- Ginseng Biological Effects and Applications
- Vitamin C and Antioxidants Research
- Hepatitis B Virus Studies
- Biochemical effects in animals
- RNA Interference and Gene Delivery
- Alcohol Consumption and Health Effects
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Phagocytosis and Immune Regulation
University of Patras
2023-2025
National and Kapodistrian University of Athens
2016-2025
BACKGROUND Previous studies have shown that baseline hematologic characteristics concerning or influencing red blood cell (RBC) properties might affect storage lesion development in individual donors. This study was conducted to evaluate whether variation hemolysis, microparticle accumulation, phosphatidylserine (PS) exposure, and other lesion–associated variables be a function of the prestorage biologic profiles donor. STUDY DESIGN AND METHODS Ten eligible, regular donors were paired...
Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest malignancies worldwide, while it persists as fourth most prevalent cause cancer-related death in United States America. Although there are several novel therapeutic strategies for approach this intensely aggressive tumor, remains a clinical challenge, hard to identify early stages, due its asymptomatic course. A diagnosis usually established when disease already late chemoresistance constitutes an obstacle optimal management...
BACKGROUND Oxidative stress orchestrates a significant part of the red blood cell (RBC) storage lesion. Considering tremendous interdonor variability observed in “storability,” namely, capacity RBCs to sustain lesion, this study aimed at elucidation donor‐specific factors that affect redox homeostasis during standard systems. STUDY DESIGN AND METHODS The hematologic profile regular donors (n = 78) was evaluated by biochemical analysis 48 different variables, including vivo hemolysis and...
Blood donor genetics and lifestyle affect the quality of red blood cell (RBC) storage. Heterozygotes for beta thalassemia (bThal+) constitute a non-negligible proportion donors in Mediterranean other geographical areas. The unique hematological profile bThal+ could capacity enduring storage stress, however, storability RBC is largely unknown. In this study, from 18 were stored cold profiled primary (hemolysis) secondary (phosphatidylserine exposure, potassium leakage, oxidative stress)...
Abstract Background Storage of packed red blood cells (RBCs) for transfusion leads to biochemical and morphological changes, increasing hemolysis risk. Urate levels in bags at donation contribute the molecular heterogeneity hemolytic propensity stored RBCs. However, studies date have been underpowered investigate scale contribution donor demographics genetics urate across donations. Study Design Methods were measured 13,091 RBC units from REDS study. Characteristics tested included...
Background: The platelet functionality of cold-stored platelets remains a subject debate. Our aim was to investigate the effect temperature on hemostatic properties stored platelets. Methods: Ten split pooled at cold and room were evaluated in vitro storage days 1, 5, 10, 15 for metabolic, physiological, vesiculation parameters, as well their profile using rotational thromboelastometry (ROTEM®). Results: integrity better preserved platelets, lower lactate dehydrogenase levels documented...
Background Several factors contribute to the manifestation of red blood cell (RBC) storage lesions, with one most interesting being donor variation effect. Since many haematological characteristics donors are sex-dependent, sex hormones and their age-dependent may affect profile RBCs. Materials methods Fresh from 200 healthy male female underwent haematological, biochemical physiological analysis. Three selected groups (men, n=8; pre-menopausal women, post-menopausal n=4) exhibiting as...
Objective: In glucose 6-phosphate dehydrogenase (G6PD) deficiency, decreased NADPH regeneration in the pentose phosphate pathway and subnormal levels of reduced glutathione result insufficient anti-oxidant defense, increased susceptibility red blood cells (RBCs) to oxidative stress, acute hemolysis following exposure pro-oxidant drugs infections. Despite fact that redox disequilibrium is a prominent feature RBC storage lesion it has been reported G6PD deficient RBCs store well, at least...
Redox imbalance and oxidative stress have emerged as generative causes of the structural functional degradation red blood cells (RBC) that happens during their hypothermic storage at banks. The aim present study was to examine whether antioxidant enhancement stored RBC units following uric (UA) and/or ascorbic acid (AA) supplementation can improve storability well post-transfusion phenotypes recovery by using in vitro animal models, respectively. For this purpose, 34 leukoreduced CPD/SAGM...
Proteasomes are proteolytic complexes with prominent roles in the control of protein homeostasis and cellular viability. However, little is known about effects storage glucose-6-phosphate dehydrogenase deficiency (G6PD-) on activity topology red blood cell (RBC) proteasomes.We investigated concentration (by GeLC-MS proteomics analysis immunoblotting), using peptide substrates proteasome inhibitors), subcellular/extracellular distribution (following fractionation isolation extracellular...
Genetic characteristics of blood donors may impact the storability products. Despite higher basal stress, red cells (RBCs) from eligible that are heterozygous for beta-thalassemia traits (βThal+) possess a differential nitrogen-related metabolism, and cope better with storage stress compared to control. Nevertheless, not much is known about how impacts proteome membrane extracellular vesicles (EVs) in βThal+. For this purpose, RBC units twelve βThal+ were studied through proteomics,...
BACKGROUND Previous investigations in leukoreduced units of red blood cells (RBCs) mannitol additive solution revealed the close association uric acid (UA) levels vivo with susceptibility RBCs to storage lesion markers. In this study, we examined whether UA has a similar correlation capability cope oxidative provocations under different conditions, namely, CPDA‐1 and absence leukoreduction. STUDY DESIGN AND METHODS The UA‐dependent antioxidant capacity supernatant was measured...
Red blood cells (RBCs) release hemoglobin (Hb)-containing extracellular vesicles (EVs) throughout their lifespan in the circulation, and especially during senescence, by spleen-facilitated vesiculation of membrane. During ex vivo aging under bank conditions, RBCs lose Hb, both soluble form inside EVs that accumulate as a part storage lesion supernatant unit. Spontaneous hemolysis are increasingly promoted duration, but little is known about any physiological linkage between them. In present...
This article contains data on the variation in several physiological parameters of red blood cells (RBCs) donated by eligible glucose-6-phosphate dehydrogenase (G6PD) deficient donors during storage standard bank conditions compared to control, G6PD sufficient (G6PD(+)) cells. Intracellular reactive oxygen species (ROS) generation, cell fragility and membrane exovesiculation were measured RBCs throughout period, with or without stimulation oxidants, supplementation N-acetylcysteine energy...