A5087030693- Erythrocyte Function and Pathophysiology
- Hemoglobinopathies and Related Disorders
- Blood transfusion and management
- Blood groups and transfusion
- Hemoglobin structure and function
- Extracellular vesicles in disease
- Blood properties and coagulation
- Heme Oxygenase-1 and Carbon Monoxide
- Neonatal Health and Biochemistry
- Iron Metabolism and Disorders
- MicroRNA in disease regulation
- Erythropoietin and Anemia Treatment
- Biochemical effects in animals
- Blood donation and transfusion practices
- Free Radicals and Antioxidants
- Circular RNAs in diseases
- Platelet Disorders and Treatments
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Phagocytosis and Immune Regulation
- Ginseng Biological Effects and Applications
- Vitamin C and Antioxidants Research
- RNA Interference and Gene Delivery
- Alcohol Consumption and Health Effects
- Immune Cell Function and Interaction
- Hepatitis B Virus Studies
University of Patras
2023-2025
National and Kapodistrian University of Athens
2017-2025
University of West Attica
2023
Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest malignancies worldwide, while it persists as fourth most prevalent cause cancer-related death in United States America. Although there are several novel therapeutic strategies for approach this intensely aggressive tumor, remains a clinical challenge, hard to identify early stages, due its asymptomatic course. A diagnosis usually established when disease already late chemoresistance constitutes an obstacle optimal management...
Blood donor genetics and lifestyle affect the quality of red blood cell (RBC) storage. Heterozygotes for beta thalassemia (bThal+) constitute a non-negligible proportion donors in Mediterranean other geographical areas. The unique hematological profile bThal+ could capacity enduring storage stress, however, storability RBC is largely unknown. In this study, from 18 were stored cold profiled primary (hemolysis) secondary (phosphatidylserine exposure, potassium leakage, oxidative stress)...
Abstract Background Storage of packed red blood cells (RBCs) for transfusion leads to biochemical and morphological changes, increasing hemolysis risk. Urate levels in bags at donation contribute the molecular heterogeneity hemolytic propensity stored RBCs. However, studies date have been underpowered investigate scale contribution donor demographics genetics urate across donations. Study Design Methods were measured 13,091 RBC units from REDS study. Characteristics tested included...
Background: The platelet functionality of cold-stored platelets remains a subject debate. Our aim was to investigate the effect temperature on hemostatic properties stored platelets. Methods: Ten split pooled at cold and room were evaluated in vitro storage days 1, 5, 10, 15 for metabolic, physiological, vesiculation parameters, as well their profile using rotational thromboelastometry (ROTEM®). Results: integrity better preserved platelets, lower lactate dehydrogenase levels documented...
Background Several factors contribute to the manifestation of red blood cell (RBC) storage lesions, with one most interesting being donor variation effect. Since many haematological characteristics donors are sex-dependent, sex hormones and their age-dependent may affect profile RBCs. Materials methods Fresh from 200 healthy male female underwent haematological, biochemical physiological analysis. Three selected groups (men, n=8; pre-menopausal women, post-menopausal n=4) exhibiting as...
Redox imbalance and oxidative stress have emerged as generative causes of the structural functional degradation red blood cells (RBC) that happens during their hypothermic storage at banks. The aim present study was to examine whether antioxidant enhancement stored RBC units following uric (UA) and/or ascorbic acid (AA) supplementation can improve storability well post-transfusion phenotypes recovery by using in vitro animal models, respectively. For this purpose, 34 leukoreduced CPD/SAGM...
Proteasomes are proteolytic complexes with prominent roles in the control of protein homeostasis and cellular viability. However, little is known about effects storage glucose-6-phosphate dehydrogenase deficiency (G6PD-) on activity topology red blood cell (RBC) proteasomes.We investigated concentration (by GeLC-MS proteomics analysis immunoblotting), using peptide substrates proteasome inhibitors), subcellular/extracellular distribution (following fractionation isolation extracellular...
Genetic characteristics of blood donors may impact the storability products. Despite higher basal stress, red cells (RBCs) from eligible that are heterozygous for beta-thalassemia traits (βThal+) possess a differential nitrogen-related metabolism, and cope better with storage stress compared to control. Nevertheless, not much is known about how impacts proteome membrane extracellular vesicles (EVs) in βThal+. For this purpose, RBC units twelve βThal+ were studied through proteomics,...
BACKGROUND Previous investigations in leukoreduced units of red blood cells (RBCs) mannitol additive solution revealed the close association uric acid (UA) levels vivo with susceptibility RBCs to storage lesion markers. In this study, we examined whether UA has a similar correlation capability cope oxidative provocations under different conditions, namely, CPDA‐1 and absence leukoreduction. STUDY DESIGN AND METHODS The UA‐dependent antioxidant capacity supernatant was measured...
Red blood cells (RBCs) release hemoglobin (Hb)-containing extracellular vesicles (EVs) throughout their lifespan in the circulation, and especially during senescence, by spleen-facilitated vesiculation of membrane. During ex vivo aging under bank conditions, RBCs lose Hb, both soluble form inside EVs that accumulate as a part storage lesion supernatant unit. Spontaneous hemolysis are increasingly promoted duration, but little is known about any physiological linkage between them. In present...
Oxidative phenomena are considered to lie at the root of accelerated senescence observed in red blood cells (RBCs) stored under standard bank conditions. It was recently shown that addition uric (UA) and/or ascorbic acid (AA) preservative medium beneficially impacts storability features RBCs related handling pro-oxidant triggers. This study constitutes next step, aiming examine links between hemolysis, redox, and metabolic parameters control supplemented RBC units different storage times....
Proteasomes are multi-catalytic complexes with important roles in protein control. Their activity stored red blood cells (RBCs) is affected by both storage time and the donor’s characteristics. However, apart from their abundancy membrane proteome, not much known about topology, activity, networking during of RBCs beta-thalassemia trait donors (βThal+). For this purpose, RBC units fourteen βThal+ were fractionated studied for proteasome distribution interactome through fluorometric...
To preserve cellular integrity and avoid bacterial growth, storage transfer of blood products follow strict guidelines in terms temperature control. We evaluated the impact ineligible warming whole donations on quality components.One-hundred twenty units (WB) from eligible donors were collected CPDA-1 stored at 4±2 °C. During shipment to processing centre, a gradual up 17 °C was recorded within period less than eight hours. The warmed processed packed red cells (PRBCs) or as WB In-bag...
The broad spectrum of beta-thalassemia ( β Thal) mutations may result in mild reduction ++ ), severe + ) or complete absence 0 beta-globin synthesis. Thal heterozygotes eligible for blood donation are “good storers” terms red cell (RBC) fragility, proteostasis and redox parameters storage lesion. However, it has not been examined if heterogeneity genetic backgrounds among Thal-trait donors affects their RBC storability profile. For this purpose, a paired analysis physiological omics was...
Blood donors with beta-thalassemia traits (βThal
Transfusion research has recently focused on the discovery of red blood cell (RBC) storage capacity biomarkers and elucidation donor variation effects. This shift focus can further strengthen personalization transfusion therapy, by revealing probable links between biology, RBC lesion profile, posttransfusion performance.We performed a paired correlation analysis osmotic fragility in freshly drawn RBCs during cold different preservative solutions at weekly intervals until unit's expiration...
The 24-hour (24 h) post-transfusion survival of donor red blood cells (RBCs) is an important marker transfusion efficacy. Nonetheless, within that period, donated RBCs may encounter challenges able to evoke rapid stress-responses. aim the present study was assess effect exposure plasma and body temperature upon stored under recipient-mimicking conditions in vitro from first hours "post-transfusion" up 24 h. For this purpose, packed seven leukoreduced CPD/SAGM units were reconstituted with...
The clarification of donor variation effects upon red blood cell (RBC) storage lesion and transfusion efficacy may open new ways for donor-recipient matching optimization. We hereby propose a "triangular" strategy studying the links comprising chain-donor, product, recipient-as exemplified in two cohorts control beta-thalassemia minor (βThal+) donors (n = 18 each). It was unraveled that RBC osmotic fragility caspase-like proteasomal activity can link both to post-storage states. In case...