James E. Norton
A5105984693- Gut microbiota and health
- Sepsis Diagnosis and Treatment
- S100 Proteins and Annexins
- Microbial Inactivation Methods
- Immune Response and Inflammation
- Wildlife Ecology and Conservation
- Genetic and phenotypic traits in livestock
- Genetic diversity and population structure
- Clusterin in disease pathology
- Immune cells in cancer
- Clostridium difficile and Clostridium perfringens research
- Rangeland Management and Livestock Ecology
- Transgenic Plants and Applications
- Primate Behavior and Ecology
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Immunotherapy and Biomarkers
- Genetic Associations and Epidemiology
- Lipid Membrane Structure and Behavior
- Posttraumatic Stress Disorder Research
- Biomedical Text Mining and Ontologies
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Glaucoma and retinal disorders
- Asthma and respiratory diseases
- Adipokines, Inflammation, and Metabolic Diseases
New York/New Jersey VA Health Care Network
2024
Merck & Co., Inc., Rahway, NJ, USA (United States)
2021-2023
Quantitative BioSciences
2023
North Bristol NHS Trust
2023
Merck (Germany)
2023
Dana-Farber Cancer Institute
2019
Northwestern University
2004-2013
University of Illinois Chicago
2004
Acute lung injury and acute respiratory distress syndrome are common clinical syndromes resulting largely from the accumulation of inability to clear pulmonary edema, due alveolar epithelium. Gene therapy may represent an important alternative for treatment prevention these diseases by restoring epithelial function. We have recently developed electroporation strategy transfer genes lungs mice, with high efficiency low inflammation.We asked whether electroporation-mediated encoding subunits...
Sepsis is a life-threatening condition caused by dysregulated host response to infection. Despite continued efforts understand the pathophysiology of sepsis, no effective therapies are currently available. While singular components aberrant immune have been investigated, comprehensive studies linking different data layers lacking. Using an integrated systems immunology approach, we evaluated neutrophil phenotypes and concomitant changes in cytokines metabolites patients with sepsis. Our...
Abstract Background Posttraumatic stress disorder (PTSD) and traumatic brain injury (TBI) are associated with self-reported problems cognition as well risk for Alzheimer’s disease related dementias (ADRD). Overlapping symptom profiles observed in cognitive disorders, psychiatric environmental exposures (e.g., head injury) can complicate the detection of early signs ADRD. The interplay between PTSD, injury, subjective (self-reported) concerns genetic ADRD is also not understood, particularly...
Elevated Lipoprotein(a) [Lp(a)] is a causal risk factor for atherosclerotic cardiovascular disease, but the mechanisms of are debated. Studies have found inconsistent associations between Lp(a) and measurements atherosclerosis. We aimed to assess relationship Lp(a), low-density lipoprotein cholesterol (LDL-C) coronary artery plaque severity.
We show that simultaneous study of stool and nasopharyngeal microbiome reveals divergent timing patterns maturation, suggesting local mucosal factors may influence composition in the gut respiratory system. Antibiotic exposure early life as occurs commonly, have an adverse effect on vaccine responsiveness. Abundance and/or bacteria with machinery to produce lipopolysaccharide-a toll-like receptor 4 agonist-may positively affect future protection, potentially by acting a natural adjuvant. The...
Abstract Baird's tapir, Tapirus bairdii , is an endangered, large, Central American mammal whose shy behaviour, solitary social structure and preference for deep tropical forests make it difficult to study using traditional field biology techniques. Despite great concern regarding its conservation status, very little known about population structure, mating system or dispersal patterns, information needed designing appropriate management plans. Molecular genetic approaches can be of use in...
Abstract The objectives of this study were to assess the level genetic variability and population differentiation within captive populations an endangered large mammal, Baird's tapir ( Tapirus bairdii ). We genotyped 37 animals from North American (NA) Central (CA) zoos conservation ranches using six polymorphic microsatellite loci. Standard indices (allelic richness diversity, heterozygosity) estimated compared between populations, wild samples. In addition, we evaluated levels Weir...
Emerging evidence demonstrates a connection between microbiome composition and suboptimal response to vaccines (vaccine hyporesponse). Harnessing the interaction microbes immune system could provide novel therapeutic strategies for improving vaccine response. Currently we do not fully understand mechanisms dynamics by which influences Using both mouse non-human primate models, report that short-term oral treatment with single antibiotic (vancomycin) results in disruption of gut this...
There is mounting evidence that the microbiome plays a critical role in training and maturation of host immune system. Pre-clinical clinical studies have shown perturbation correlated with sub-optimal responses to vaccines cancer immunotherapy. As such, identifying species commensal bacteria capable modulating immunological outcomes considerable interest. Currently, lack reliable primary cell-based assays differentiating immuno-modulatory properties various major limitation. Here, we...
Abstract The Phenome-Wide Association Study (PheWAS) is increasingly used to broadly screen for potential treatment effects, e.g., IL6R variant as a proxy antagonists. This approach offers an opportunity address the limited power in clinical trials study differential effects across patient subgroups. However, methods exist efficiently test differences subgroups thousands of multiple comparisons generated part PheWAS. In this study, we developed that maximizes heterogeneous genotype–phenotype...
Abstract Emerging evidence demonstrates a connection between microbiome composition and suboptimal response to vaccines (vaccine hyporesponse). Harnessing the interaction microbes immune system could provide novel therapeutic strategies for improving vaccine response. Currently we do not fully understand mechanisms dynamics by which influences Using both mouse non-human primate models, report that short-term oral treatment with single antibiotic (vancomycin) results in disruption of gut this...
Abstract Psoriasin (S100A7) is a potent antimicrobial peptide produced by epithelial cells. Previous work our group has demonstrated significant reduction in both the expression of S100A7, and activation STAT3 (pSTAT3) tissue samples from patients with chronic rhinosinusitis nasal polyps (CRSwNP) compared controls. To investigate whether these two independent observations were linked, we examined relationship between pSTAT3 S100A7 primary human bronchial (NHBE) cells (NEC). NHBEs or NECs...
Abstract Background Genome-wide Association Studies (GWAS) aims to uncover the link between genomic variation and phenotype. They have been actively applied in cancer biology investigate associations variations phenotypes, such as susceptibility certain types of predisposed responsiveness specific treatments. Since GWAS primarily focuses on finding individual there are limitations understanding mechanisms by which phenotypes cooperatively affected more than one variation. Results This paper...
Abstract Sepsis is a life-threatening condition caused by an aberrant immune response to infection and subsequent organ dysfunction. Further, due shared mechanisms such as severe inflammation endothelial dysfunction, sepsis remains the most common etiology in acute respiratory distress syndrome (ARDS) patients. Despite multiple efforts identify distinct signature support development of new therapeutics, significant unmet clinical need. While singular components dysregulated have been...
Abstract Background The majority of cases Dent’s disease are caused by pathogenic variants in the CLCN5 gene, which encodes a voltage-gated chloride ion channel (ClC-5), resulting proximal tubular dysfunction. We present three members same family and one unrelated paediatric patient with insertion-deletion variant. identification these patients positive familial segregation led to re-classification this variant from unknown significance likely pathogenicity. Case presentation A 41 year old...
Electroporation is an efficient and safe technique to deliver naked DNA both cells in culture living animals. Application of brief square wave pulses induces transient pores open the plasma membrane, allowing enter cytoplasm directly. We have used this approach plasmid murine lung found high level gene expression with almost undetectable inflammation or injury. By contrast, cationic lipid mediated transfer routinely inflammatory responses characterized by increased levels cytokines. This...
Abstract Sepsis is a life-threatening condition caused by dysregulated host response to infection. Despite continued efforts understand the pathophysiology of sepsis, no effective therapies are currently available. While singular components aberrant immune have been investigated, comprehensive studies linking different data layers lacking. Using an integrated systems immunology approach, we evaluated neutrophil phenotypes and concomitant changes in cytokines metabolites sepsis patients. Our...