A5033218957- Sulfur Compounds in Biology
- Adipose Tissue and Metabolism
- Neuroscience and Neuropharmacology Research
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Mitochondrial Function and Pathology
- Ion channel regulation and function
- Neuroscience of respiration and sleep
- Nitric Oxide and Endothelin Effects
- Muscle metabolism and nutrition
- Cardiac electrophysiology and arrhythmias
- Eicosanoids and Hypertension Pharmacology
- Advanced Glycation End Products research
- Fatty Acid Research and Health
- Biochemical effects in animals
- Polyamine Metabolism and Applications
- Lipid metabolism and biosynthesis
- Pancreatic function and diabetes
- Calpain Protease Function and Regulation
- Cardiac Ischemia and Reperfusion
- Kruppel-like factors research
- Alcohol Consumption and Health Effects
- Magnesium in Health and Disease
- Cannabis and Cannabinoid Research
- Coenzyme Q10 studies and effects
Harbin Medical University
2015-2024
Research Institute of General Pathology and Pathophysiology, the Russian Academy of Medical Sciences
2013
Excessive autophagy induced by extravagant oxidative stress is the main reason for diabetes-induced vascular endothelial cells dysfunction. Hydrogen sulfide (H2S) has anti-oxidative effects but its regulation on excessive of unclear. In this study, aorta db/db mice (28 weeks old) and rat aortic (RAECs) treated with 40 mM glucose 500 μM palmitate acted as type II diabetic animal cellular models, respectively, 100 μMNaHS was used an exogenous H2S donor. The apoptosis level measured terminal...
Hydrogen sulfide (H2S), an important gasotransmitter, regulates cardiovascular functions. Mitochondrial damage induced by the overproduction of reactive oxygen species (ROS) results in myocardial injury with a diabetic state. The purpose this study was to investigate effects exogenous H2S on mitophagy formation cardiomyopathy. In study, we found that could improve cardiac functions, reduce mitochondrial fragments and ROS levels, enhance respiration chain activities inhibit apoptosis hearts...
Excessive ROS accumulation contributes to cardiac injury in type 2 diabetes mellitus. Hydrogen sulfide (H2S) is a vital endogenous gasotransmitter alleviate damage diabetic cardiomyopathy (DCM). However, the underlying mechanisms remain unclear. In this study, we investigated effects of NaHS administration db/db mice via intraperitoneal injection for 20 weeks and treatment high glucose (HG), palmitate (PA) HL-1 cardiomyocytes 48 h, respectively. H2S levels were decreased hearts exposed HG...
Endothelial cell dysfunction is one of the main reasons for type II diabetes vascular complications. Hydrogen sulphide (H2 S) has antioxidative effect, but its regulation on mitochondrial dynamics and mitophagy in aortic endothelial cells under hyperglycaemia hyperlipidaemia unclear. Rat (RAECs) were treated with 40 mM glucose 200 μM palmitate to imitate endothelium hyperlipidaemia, 100 NaHS was used as an exogenous H2 S donor. Firstly, we demonstrated that high decreased production CSE...
Background: Oxidative stress inducing hyperglycemia and high glucose play an important role in the development of cardiac fibrosis associated with diabetic cardiomyopathy. The endogenous gasotransmitter hydrogen sulfide (H2S) can act a cytoprotective manner. However, whether H2S could inhibit fibrotic process is unclear. purpose our study was to examine underlying mechanisms behind Methods: Diabetic cardiomyopathy induced rats by injection streptozotocin (STZ). Cardiac proliferation rat...
Background/Aims: Intracellular calcium concentration ([Ca2+]i) homeostasis, an initial factor of cardiac hypertrophy, is regulated by the calcium-sensing receptor (CaSR) and associated with formation autolysosomes. The aim this study was to investigate role Calhex231, a CaSR inhibitor, on hypertrophic response via autophagy modulation. Methods: Cardiac hypertrophy induced transverse aortic constriction (TAC) in 40 male Wistar rats, while 10 rats underwent sham operation served as controls....
Aims: Alterations in calcium homeostasis the intracellular endo/sarcoplasmic reticulum (ER/SR) and mitochondria of cardiomyocytes cause cell death via SR mitochondrial apoptotic pathway, contributing to ventricular dysfunction. However, role calcium-sensing receptor (CaR) cardiac hypertrophy heart failure has not been studied. This study examined possible involvement CaR pathway an experimental model failure. Methods Results: In Wistar rats, were induced by subcutaneous injection...
The upregulation of reactive oxygen species (ROS) is a primary cause cardiomyocyte apoptosis in diabetes cardiomyopathy (DCM). Mitofusin-2 (Mfn-2) key protein that bridges the mitochondria and endoplasmic reticulum (ER). Hydrogen sulfide (H2S)-mediated cardioprotection related to antioxidant effects. present study demonstrated H2S inhibited interaction between ER mitochondrial apoptotic pathway. This investigated cardiac function, ultrastructural changes mitochondria, rate using TUNEL,...
Background and Purpose Hydrogen sulfide (H 2 S) plays important roles as a gasotransmitter in pathologies. Increased expression of the E3 ubiquitin ligase, muscle RING finger‐1 (MuRF1), may be involved diabetic cardiomyopathy. Here we have investigated whether how exogenous H S alleviates cardiac degradation through modifications MuRF1 S‐sulfhydration db/db mice. Experimental Approach Neonatal rat cardiomyocytes were treated with high glucose (40 mM), oleate (100 μM), palmitate (400 NaHS μM)...
Hydrogen sulfide (H2S), a gaseous molecule, is involved in modulating multiple physiological functions, such as antioxidant, antihypertension, and the production of polysulfide cysteine. H2S may inhibit reactive oxygen species generation ATP through respiratory chain enzyme activities; however, mechanism this effect remains unclear. In study, db/db mice, neonatal rat cardiomyocytes, H9c2 cells treated with high glucose, oleate, palmitate were used animal cellular models type 2 diabetes. The...
Hydrogen sulfide (H<sub>2</sub>S) plays physiological roles in vascular tone regulation, cytoprotection, and ATP synthesis. HMG-CoA reductase degradation protein (Hrd1), an E3 ubiquitin ligase, is involved trafficking. H<sub>2</sub>S may play a role controlling fatty acid uptake diabetic cardiomyopathy (DCM) manner correlated with modulation of Hrd1 S-sulfhydration; however, this remains to be elucidated. The aim the present study was examine whether can attenuate lipid accumulation explain...
Aims: Calcium-sensing receptor (CaR) acts as a G protein coupled that mediates the increase of intracellular Ca2+ concentration. The expression CaR has been confirmed in various cell types, including cardiomyocytes, smooth muscle cells, neurons and vascular endothelial cells. However, whether is expressed functions cardiac fibroblasts remained unknown. present study investigated played role fibroblast proliferation extracellular matrix (ECM) secretion, both cultured rat neonatal model...
Ischemic preconditioning (IPC) strongly protects against myocardial ischemia reperfusion (IR) injury. However, IPC protection is ineffective in aged hearts. Exercise training reduces the incidence of age-related cardiovascular disease and upregulates ornithine decarboxylase (ODC)/polyamine pathway. The aim this study was to investigate whether exercise can reestablish hearts linked restoration cardiac polyamine pool. Rats aging 3 or 18 months perform treadmill exercises with without gradient...
Abstract Diabetic cardiomyopathy (DCM) is a serious complication of diabetes. Hydrogen sulphide (H 2 S), newly found gaseous signalling molecule, has an important role in many regulatory functions. The purpose this study to investigate the effects exogenous H S on autophagy and its possible mechanism DCM induced by type II diabetes (T2DCM). In study, we that sodium hydrosulphide (NaHS) attenuated augment left ventricular (LV) mass increased LV volume, decreased reactive oxygen species (ROS)...
Vascular smooth muscle cell (VSMC) proliferation in response to hyperglycemia is an important process the development of arterial vessel hyperplasia. The shape change mitochondria dynamic and closely related fission fusion. Hydrogen sulfide (H2S) was confirmed have anti-oxidative, anti-inflammatory anti-proliferative effects. However, little it known about its effects on mitochondrial morphology induced by hyperglycemia. aim study demonstrate that H2S inhibits VSMC through regulating...
Diabetic cardiomyopathy (DCM) is a serious complication of diabetes mellitus that eventually progresses to heart failure. The sarco(endo)plasmic reticulum calcium ATPase 2a (SERCA2a), an important pump in cardiomyocytes, closely related myocardial systolic-diastolic function. In mammalian cells, hydrogen sulfide (H2S), as second messenger, antioxidant, and sulfurizing agent, involved diverse biological processes. Despite the importance H2S for protection against DCM, mechanisms remain poorly...
Abstract Communication between the SR (sarcoplasmic reticulum, SR) and mitochondria is important for cell survival apoptosis. The supplies Ca 2+ directly to via inositol 1,4,5-trisphosphate receptors (IP 3 Rs) at close contacts two organelles referred as mitochondrion-associated ER membrane (MAM). Although it has been demonstrated that CaR (calcium sensing receptor) activation involved in intracellular calcium overload during hypoxia/reoxygenation (H/Re), role of cardiomyocyte apoptotic...
Abstract Accumulation of lipid droplets (LDs) induces cardiac dysfunctions in type 2 diabetes patients. Recent studies have shown that hydrogen sulphide (H S) ameliorates functions db/db mice, but its regulation on the formation LDs tissues is unclear. Db/db mice were injected with NaHS (40 μmol·kg ‐1 ) for twelve weeks. H9c2 cells treated high glucose mmol/L), oleate (200 µmol/L), palmitate µmol/L) and (100 48 hours. Plasmids overexpression wild‐type Hrd1 mutated at Cys115 constructed. The...
Abstract: The calcium‐sensing receptor (CaR) is a G protein‐coupled receptor. CaR stimulation elicits phospholipase C‐mediated inositol triphosphate formation, leading to an elevation in the level of intracellular calcium released from endoplasmic reticulum (ER). Depletion ER Ca 2+ leads stress, which thought induce apoptosis. Intracellular overload‐induced apoptosis cardiac myocytes during hypoxia–reoxygenation (H/Re) has been demonstrated. However, links between CaR, stress and H/Re are...