A5051605062- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Immune Cell Function and Interaction
- Liver Disease Diagnosis and Treatment
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Virus-based gene therapy research
- Cytokine Signaling Pathways and Interactions
- Cancer, Stress, Anesthesia, and Immune Response
- Biodiesel Production and Applications
- Peroxisome Proliferator-Activated Receptors
- Supercapacitor Materials and Fabrication
- Vascular Procedures and Complications
- Cardiac, Anesthesia and Surgical Outcomes
- Proteoglycans and glycosaminoglycans research
- Renal cell carcinoma treatment
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Nutrition and Health in Aging
- Aortic aneurysm repair treatments
- Immune cells in cancer
- Liver physiology and pathology
- Infectious Aortic and Vascular Conditions
- COVID-19 Clinical Research Studies
- Adipokines, Inflammation, and Metabolic Diseases
University Medical Center Hamburg-Eppendorf
2020-2025
Universität Hamburg
2020-2025
Technical University of Darmstadt
2019
University Medical Centre Mannheim
2018
Heidelberg University
2018
The liver is one of the organs most commonly affected by metastasis. presence metastases has been reported to be responsible for an immunosuppressive microenvironment and diminished immunotherapy efficacy. Herein, we aimed investigate role IL-10 in metastasis determine how its modulation could affect efficacy vivo.
Abstract Background A multicentre study was conducted to investigate the impact of sarcopenia as an independent predictor oncological outcome after radical cystectomy for bladder cancer. Methods In total, 500 patients with available digital computed tomography scans abdomen obtained within 90 days before surgery were identified. The lumbar skeletal muscle index measured using pre‐operative tomography. Cancer‐specific survival (CSS) and overall (OS) estimated Kaplan–Meier curves. Predictors...
A novel green lubricating oil additive (carbon quantum dot (CQD) particle-doped nickel (Ni-CQD)) was synthesized from citric acid and acetate. The effects of CQD Ni-CQD nanoparticles on the tribological behaviors polyethylene glycol (PEG200) were investigated under different loads reciprocation speeds. results indicate that particles can both enhance lubrication properties PEG200. However, enhanced more than plain did. average friction coefficient wear rate PEG200 containing 2 wt% Ni-CQDs...
Forced metastasis models, those in which the step of intravasation is bypassed, can be used to investigate mechanisms underlying and evaluate potential therapeutic targets. Here, we present a protocol for using three forced models lung liver generate within 3-4 weeks approximately 99% injected mice. We describe steps cancer cell preparation, mouse analgesia anesthesia; injecting through intrasplenic, intraportal, intravenous techniques; daily evaluation metastasis. For complete details on...
Metastasis is a cancer-related systemic disease and responsible for the greatest mortality rate among cancer patients. Interestingly, interaction between immune system cells seems to play key role in metastasis formation target organ. However, this complex network only partially understood. We previously found that IL-22 produced by tissue resident iNKT17 promotes cell extravasation, early step of metastasis. Based on these data, we aimed here decipher last formation. levels were increased...
Abstract The lung is one of the most frequently metastasized organs from various cancer entities, especially colorectal (CRC). occurrence metastasis correlates with worse prognosis in CRC patients. Here, we aimed to investigate role IL-10 development and identify cellular source target cells during metastatic establishment. To induce mice, injected MC38 murine colon intravenously. Mice Il10 -deficiency were used test IL-10. burden was assessed both macroscopically histologically. IL-10-...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which is responsible for eliciting Coronavirus disease 2019 (COVID-19) still challenges healthcare services worldwide. While many patients only suffer from mild symptoms, with some pre-existing medical conditions are at a higher risk detrimental course of disease. However, the underlying mechanisms determining partially understood. One key factor influencing severity described to be immune-mediated. In this report, we describe...
To investigate underlying mechanisms for cancer metastasis and promising therapies in animal models, spontaneous models can be used to recreate development. Here, we present three mouse of lung and/or liver induction. We describe steps cell preparation, analgesia, injection techniques (subcutaneous, intracecal, intramucosal). then detail procedures evaluating metastasis. Most these generate a time span 4 weeks the majority injected mice. For complete details on use execution this protocol,...
BackgroundInsufficient remnant liver volume (RLV) after the resection of hepatic malignancy could lead to failure and mortality. Portal vein ligation (PVL) prior hepatectomy is subsequently introduced increase improve outcome malignancy. IL-22 has previously been reported promote regeneration, while facilitating tumor development in via Steap4 upregulation. Here we performed PVL mouse models study role regeneration post-PVL.MethodsLiver weight was measured magnetic resonance imaging (MRI)....
This in vitro study investigates the antimicrobial efficacy of impregnation commercially available aortic endografts (EG) with rifampicin (RIF) and nanocolloidal silver.Endografts were flushed 50 mL RIF 600 mg, 70 a silver-based aqueous solution (AG), or phosphate-buffered saline (PBS) over 15 minutes. Endografts then retrieved from sheath cut 1 × cm sized graft units (n = 80 each impregnation), which incubated for hour separately inoculates containing 106 103 bacteria per milliliter...
The transplantation model provides the opportunity to assess relevance of a molecule interest for tumor cell extravasation by using respective genetically modified donor animal. Here, we present protocol orthotopic single-lung in mice as tool lung metastasis studies. We describe steps animal preparation, transplantation, and injection. then detail procedures direct comparison spreading between left naive right parenchyma. For complete details on use execution this protocol, please refer...
Differential glycosylation, marked by the presence of truncated O-glycans, is a distinctive feature epithelial-derived cancers. However, there notable gap in research regarding expression Tn and STn antigens esophageal adenocarcinoma (EAC). To address this, we employed commercially available antibodies, previously validated for antigens, to analyze two cohorts EAC tissues. Initially, large-area tissue sections from formalin-fixed paraffin-embedded (FFPE) corresponding healthy tissues were...
Zusammenfassung Die wichtigste Komplikation nach Pankreatoduodenektomie ist die klinisch relevante Pankreasfistel. Um Rate an Komplikationen zu senken, eine routinierte und standardisierte Operationstechnik zur Anlage der Pankreatojejunostomie notwendig. im multimodalen Setting Goldstandard Behandlung des lokal begrenzten Pankreaskopfkarzinoms weiterer Pathologien. Das robotische Verfahren bietet als innovatives minimalinvasives Vorteile bez. Morbidität Ergonomie. Nach Resektionsphase folgt...
The immune system plays a pivotal role in cancer progression. Interleukin 22 binding protein (IL-22BP), natural antagonist of the cytokine interleukin (IL-22) has been shown to control progression colorectal (CRC). However, IL-22BP process metastasis formation remains unknown.We used two different murine vivo models using MC38 and LLC cell lines studied lung liver after intracaecal or intrasplenic injection cells. Furthermore, IL22BP expression was measured clinical cohort CRC patients...
Background Insufficient remnant liver volume (RLV) after the resection of hepatic malignancy could lead to failure and mortality. Portal vein ligation (PVL) prior hepatectomy is subsequently introduced increase improve outcome malignancy. IL-22 has previously been reported promote regeneration, while facilitating tumor development in via Steap4 upregulation. Here we performed PVL mouse models study role regeneration post-PVL. Methods Liver weight was measured magnetic resonance imaging...