A5063585218- Bacterial Genetics and Biotechnology
- COVID-19 Clinical Research Studies
- Genomics and Phylogenetic Studies
- SARS-CoV-2 and COVID-19 Research
- Bacillus and Francisella bacterial research
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Respiratory Support and Mechanisms
- RNA and protein synthesis mechanisms
- Biochemical Acid Research Studies
- Tardigrade Biology and Ecology
- Single-cell and spatial transcriptomics
- Neuroblastoma Research and Treatments
- interferon and immune responses
- Liver Disease Diagnosis and Treatment
- Microbial metabolism and enzyme function
- Parasitic Infections and Diagnostics
- Genetic Associations and Epidemiology
- Nutritional Studies and Diet
- Signaling Pathways in Disease
- Immune cells in cancer
- Enzyme Production and Characterization
- Metalloenzymes and iron-sulfur proteins
- Respiratory viral infections research
- Inflammation biomarkers and pathways
Medizinische Hochschule Hannover
2021-2025
Hochschule Hannover
2025
Université Paris-Est Créteil
2024
Assistance Publique – Hôpitaux de Paris
2024
Inserm
2024
Oil Tools Of Norway (Norway)
2021
University of Göttingen
2011-2017
Universitätsmedizin Göttingen
2008-2009
Zentrum für Kinderheilkunde
2008
University of Wisconsin–Milwaukee
2007
Aging affects human immune system functionality, increasing susceptibility to immune-mediated diseases. While gene expression programs accurately reflect function, their relationship with biological aging and health status remains unclear. Here we developed robust, cell-type-specific clocks (sc-ImmuAging) for the myeloid lymphoid cell populations in circulation within peripheral blood mononuclear cells, using single-cell RNA-sequencing data from 1,081 healthy individuals aged 18 97 years....
Abstract Background The production of enzymes by an industrial strain requires a complex adaption the bacterial metabolism to conditions within fermenter. Regulatory events process result in dynamic change transcriptional activity genome. This network genes is orchestrated proteins as well regulatory RNA elements. Here we present RNA-Seq based study considering selected phases industry-oriented fermentation Bacillus licheniformis . Results A detailed analysis 20 strand-specific datasets...
Abstract Stanniocalcin 2 (STC2) is a secreted glycoprotein of as yet unknown functions. We investigated STC2 in human neuroblastoma, the most common solid extra‐cranial tumor infancy. In primary samples, we found that expression associated with metastatic Stages 4 and 4s MYCN expression. vitro, however, demonstrate cell proliferation reduced by due to an increase basal apoptosis rate transfected cells. On other hand, vitro assays showed STC2‐transfected neuroblastoma cells have increased...
SARS-CoV-2 infected patients show heterogeneous clinical presentations ranging from mild symptoms to severe respiratory failure and death. Consequently, various markers reflect this wide spectrum of disease presentations.
Prophages are viruses, which have integrated their genomes into the genome of a bacterial host. The status prophage can vary from fully intact with potential to form infective particles remnant state where only few phage genes persist. impact on properties host and therefore great interest for genomic research strain design. Here we present genome- next generation sequencing (NGS)-based approach identification activity evaluation regions. Seven or prophage-like regions were identified in...
The Gluconobacter oxydans 621H genome contains two genes (gox1122 and gox0499) that encode putative cytosolic NAD(P)-dependent aldehyde dehydrogenases. Each gene was expressed in Escherichia coli, the recombinant enzymes were purified characterized. native protein Gox1122 exhibited an apparent molecular mass of 50.1 kDa, subunit 50.5 indicating a monomeric structure enzyme. preferred substrates acetaldehyde NADP. enzyme also oxidized other short-chained aliphatic aromatic aldehydes at lower...
Strains of the species Bacillus licheniformis are widely used in biotechnology for production enzymes and antibiotics (M. Schallmey, A. Singh, O. P. Ward, Can. J. Microbiol. 50:1-17, 2004). However, research application B. strains adversely affected by poor genetic accessibility. Thus, a closer inspection natural competence licheniformis, genome strain 9945A, which derivatives known to be naturally competent (C. Thorne H. Stull, Bacteriol. 91:1012-1020, 1966), was completely sequenced...
Abstract Purpose To investigate the transcriptome of human bronchial epithelial cells (HBEC) in response to serum from patients with different degrees inflammation. Methods Serum 19 COVID-19 obtained Hannover Unified Biobank was used. At time sampling, 5 had a WHO Clinical Progression Scale (WHO-CPS) score 9 (severe illness). The remaining 14 WHO-CPS below (range 1–7), and lower illness. Multiplex immunoassay used assess inflammatory markers. culture medium HBEC supplemented 2% patient’s...
We report on genome sequencing of Oligotropha carboxidovorans strain OM4 and resequencing OM5. The genomes both are composed one chromosome two plasmids. presence plasmids in the OM5 is inconsistent with previously published sequence, for which only plasmid was described (D. Paul, S. Bridges, Burgess, Y. Dandass, M. Lawrence, BMC Genomics 11:511, 2010).
Neuroblastoma is the most frequent solid malignancy of children. The reliable prognostic factor in neuroblastoma amplification status MYCN oncogene, but exceptions from this rule have been observed. Recently we demonstrated that keratoepithelin (BIGH3, TGFBI) expression significantly reduces proliferation and invasion neuroblastomas vitro vivo. In these experiments, also observed tissue pathway inhibitor 2 (TFPI2, PP5, MSPI), a potent matrix-metalloproteinases, prominently up-regulated. As...
The species Bacillus licheniformis includes important strains that are used in industrial production processes. Currently the physiological model to adapt these processes is based on closely related organism B. subtilis. In this study we found both organisms reveal significant differences regulation of subtilisin, their main natural protease and a product fermentation We identified characterized novel antisense sRNA AprAs, which represents an RNA repressor apr, gene encoding for relevant...
Bacterial natural genetic competence – well studied in Bacillus subtilis enables cells to take up and integrate extracellularly supplied DNA into their own genome. However, little is known about development its regulation other members of the genus, although uptake machineries are routinely encoded. Auxotrophic licheniformis 9945A derivatives, obtained from repeated rounds random mutagenesis, were long develop competence. Inspection colony morphology extracellular enzyme secretion two these...
Bacillus wild-type strains are genetically difficult to manipulate, and thus, the options for rational strain investigation design limited. Here, we present a set of small conjugative shuttle vectors use in licheniformis related, accessible strains. The vector comprises modular general-purpose pV2 its derivatives pV3SDlacZ pV3lacZ. pV3 designed transcriptional translational activities regulatory regions like promoters ribosomal binding sites (RBS). has been tested investigating gene...
Natural genetic competence renders bacteria able to take up and, in case there is sufficient homology the recipient's chromosome, integrate exogenously supplied DNA. Well studied Bacillus subtilis, is-in several aspects-known be differently regulated licheniformis. We now report on identification of a novel, chromosomally encoded homolog inhibitor B. licheniformis (ComI) that has hitherto only been described as plasmid borne trait ancestral subtilis NCIB3610. Bioinformatical analysis...
Abstract Mortality of patients hospitalized with COVID-19 has remained high during the consecutive SARS-CoV-2 pandemic waves. Early discrimination at mortality risk is crucial for optimal patient care. Symmetric (SDMA) and asymmetric dimethylarginine (ADMA) have been proposed as possible biomarkers to improve prediction patients. We measured SDMA, ADMA, other L-arginine-related metabolites in 180 admitted four German university hospitals compared 127 healthy controls. Patients were treated...
Courses of SARS-CoV-2 infections are highly variable, ranging from asymptomatic to lethal COVID-19. Though research has shown that host genetic factors contribute this variability, cohort-based joint analyses variants the entire allelic spectrum in individuals with confirmed still lacking. Here, we present results whole genome sequencing 1,220 mainly vaccine-naïve infection, including 827 hospitalized COVID-19 cases. We observed presence autosomal-recessive or likely compound heterozygous...
SARS-CoV-2 infected patients show heterogeneous clinical presentations ranging from mild symptoms to severe respiratory failure and death. Consequently, various markers reflect certain disease presentations. Our cohort included moderate (n=10) COVID-19 patients, 10 healthy controls. We determined plasma levels of nine acute phase proteins by nephelometry, full-length (M65), caspase-cleaved (M30) cytokeratin 18, ADAMTS13 (a disintegrin-like metalloprotease with thrombospondin type-1 motif 13)...
The hyperactivation of the immune system could result in acute respiratory distress syndrome. We used serum from clinically well-characterized COVID-19 patients with severe (n=10) and moderate (n=9) disease to analyze which profiles levels pro- anti-inflammatory markers affect human microvascular lung endothelial (EC) bronchial epithelial (EpC) cells, <i>in vitro</i>. According cytokine/chemokine/inflammatory marker multiplex assay, most were elevated whereas statistically higher found only...