A5003750946- HIV Research and Treatment
- Viral-associated cancers and disorders
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Helicobacter pylori-related gastroenterology studies
- Immune Cell Function and Interaction
- Lymphoma Diagnosis and Treatment
- Cytomegalovirus and herpesvirus research
- Galectins and Cancer Biology
- Polyomavirus and related diseases
- HIV-related health complications and treatments
- Parvovirus B19 Infection Studies
- Veterinary medicine and infectious diseases
- Gastric Cancer Management and Outcomes
- Hepatitis C virus research
- Pneumocystis jirovecii pneumonia detection and treatment
- Eosinophilic Esophagitis
- Herpesvirus Infections and Treatments
- Immunodeficiency and Autoimmune Disorders
- CNS Lymphoma Diagnosis and Treatment
- Hepatitis B Virus Studies
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Cell Adhesion Molecules Research
- Cancer Immunotherapy and Biomarkers
Centro di Riferimento Oncologico
2013-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2005-2023
Policlinico San Matteo Fondazione
2018
MRC Epidemiology Unit
2003-2007
University of Turin
2006
A. O. Ordine Mauriziano di Torino
2006
AIDS United
1996
Abstract Helicobacter pylori , a dominant member of the gastric microbiota, shares co-evolutionary history with humans. This has led to development genetically distinct H. subpopulations associated geographic origin host and differential disease risk. Here, we provide insights into population structure as part Genome Project ( Hp GP), multi-disciplinary initiative aimed at elucidating pathogenesis identifying new therapeutic targets. We collected 1011 well-characterized clinical strains from...
Abstract Background Dual therapy (DT) with boosted protease inhibitors (bPIs) plus lamivudine has been shown to be superior bPI monotherapy in virologically suppressed patients despite previous selection of the resistance M184V mutation. We compared virological efficacy lamivudine-based DT and without a history detection. Methods retrospectively analyzed HIV-RNA ≤50 copies/mL switching at least 1 genotype ARCA database. Time failure (VF; ≥200 or 2 consecutive >50 copies/mL) treatment...
Abstract In most HIV-1-infected patients, clinical and immunological progression develops within a few years. Few infected people, termed long-term non-progressors (LTNP), remain healthy immunologically stable for long time. The factors governing the maintenance of this condition are not well known, but it is conceivable that CD8+ lymphocytes, cells play central role in controlling vitro HIV replication, may have part vivo process. aim study was to characterize phenotypic profile cytokine...
HIV infection is characterized by the reduction of CD4+, CD45RA+, CD26+, and CD28+ lymphocyte subsets in vitro production IL-2, IL-4, interferon-gamma; on contrary, chemokine usually increased. These abnormalities are only partially restored antiretroviral chemotherapy. Therapy with interleukin-2 has been proposed to restore functions immune system, but mechanisms which IL-2 exerts its activities unknown. The aim this study was define effects rIL-2 administration CD45R0+, CD26+ lymphocytes...
High-dose chemotherapy (HDC) and autologous stem cell transplantation (ASCT) are feasible effective salvage treatments for human immunodeficiency virus (HIV)-related relapse or refractory lymphoma. Among the main concerns with ASCT in HIV-infected persons is additional immune depletion caused by treatment, which could amplify preexisting deficit. The aims of our study were to assess impact conventional before treatment was administered, this population, evaluate reconstitution dynamics...
Background and objectivesGuidelines indicate a plasma HIV-1 RNA load of 500–1000 copies/mL as the minimal threshold for antiretroviral drug resistance testing. Resistance testing at lower viral levels may be useful to guide timely treatment switches, although data on clinical utility this remain limited. We report here influence probability detecting mutations (DRMs) other by routine genotypic in large multicentre European cohort, with focus tests performed <1000 copies/mL.
ABSTRACT The Helicobacter pylori chromosomal cluster of genes known as the cytotoxin-associated gene ( cag ) island may have different compositions in infecting strains. In this study, we analyzed 150 single colonies obtained from gastric biopsy specimens 10 patients infected with cagA -positive H . strains and sweep isolates (isolates harvested points plate) 6 -negative Three loci , cagE virB11 conserved glmM ureC were investigated by PCR. levels anti- anti-CagA antibodies patient sera also...
Objective To screen and characterize germline variants for E-cadherin (CDH1) in non-hereditary gastric cancer (GC) patients subjects at risk of GC. Methods 59 GCs, first degree relatives (FDRs) GC, 20 autoimmune metaplastic atrophic gastritis (AMAGs) 52 blood donors (BDs) were analyzed CDH1 by direct sequencing, structural modelling bioinformatics. Functional impact on splicing was assessed intronic mutations. E-cadherin/β-catenin immunohistochemical staining mRNA quantification using RT-PCR...
Autoimmune atrophic gastritis (AAG) is associated with an increased risk of certain types gastric cancer (GC). Helicobacter pylori (H. pylori) infection may have a role in the induction and/or maintenance AAG and GC. Toll-like receptors (TLR) are essential for H. recognition subsequent innate adaptive immunity responses. This study therefore aimed to characterize TLR polymorphisms, features bacterial flagellin A samples from patients (n = 67), GC 114) healthy donors (HD; n 97). TLR5...
Abstract This study presents the immunophenotypic and functional analysis of lymphocyte subsets obtained from peripheral blood lymphoid tissue HIV+ individuals treated with highly active anti-retroviral therapy (HAART) alone or in combination 6 million units international (MUI) s.c. IL-2. Before treatment, patients had reduced CD4 increased CD8 values impaired cytokine production by mononuclear cells (PBMC). After 24 weeks all demonstrated tissue. The use IL-2 did not promote an additional...
To assess the efficacy of therapeutic use inhibitors ribonucleotide reductase for treatment human immunodeficiency virus (HIV) type 1 infection, 7 consecutive patients were enrolled in a clinical trial involving monotherapy with hydroxyurea 8-19 weeks. During therapy, evaluated status and immunologic, hematologic, quantitative virologic parameters, including determinations viremia number provirus-containing cells by competitive polymerase chain reaction. In all patients, these parameters not...
The amino- and carboxyl-terminal globular domains of type VI collagen are composed several homologous modules similar to the A collagen-binding present in von Willebrand factor. human alpha 3(VI) chain that contributes most amino-terminal globule appears heterogeneous size as a result alternative splicing two exons (Stokes D. G., Saitta, B., Timpl, R., Chu, M.-L. (1991) J. Biol. Chem. 266, 8626-8633). In study, we report further characterization 5'-end gene show transcription initiates at...
A large number of evidences indicate that progression HIV disease is driven by an increase in viral burden. It still unclear, however, to what extent this contributed the dysregulation molecular mechanisms governing virus gene expression at transcriptional or posttranscriptional levels. To address issue, several quantitative virologic parameters (including provirus activity and splicing pattern) were analyzed individuals with nonprogressive infection compared those a matched group progressor...
SUMMARY The kinetics and effects of in vivo spontaneous apoptosis activation-induced cell death (AICD) upon CD4+ CD8+ lymphocyte subsets CD4 naive numbers were studied HIV+ subjects with pretreatment values &gt; 200/mm3, who subsequently treated for 48 weeks HAART alone or combination six cycles subcutaneous IL-2. Irrespective the type treatment, patients showed a statistically significant increase counts after 4 weeks, although subset only increased significantly IL-2-treated at end...
Atrophic autoimmune gastritis (AAG) is a condition of chronic inflammation and atrophy stomach mucosa, for which development can be partially triggered by the bacterial pathogen Helicobacter pylori (HP). HP cause variety gastric diseases, such as duodenal ulcer (DU) or cancer (GC). In this study, comparative proteomic approach was used two-dimensional fluorescence difference gel electrophoresis (DIGE) to identify differentially expressed proteins strains isolated from patients with AAG,...
A phase II study was performed to evaluate the feasibility and activity of subcutaneous (SC) interleukin-2 (IL-2) administration plus zidovudine (ZDV) didanosine (ddI) in patients with early stage HIV infection. Between October 1995 1996, 12 completed 6 cycles following scheduled therapy: ZDV ddI SC self-administration mIU IL-2 at days 1 5 8 a 28-day cycle for total (24 weeks). After cycles, received only they were observed up an additional 24 weeks. Our schedule well tolerated as outpatient...