A5066688128- Hepatitis C virus research
- Animal Virus Infections Studies
- Hepatitis B Virus Studies
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Glioma Diagnosis and Treatment
- Meningioma and schwannoma management
- Liver Disease Diagnosis and Treatment
- Epilepsy research and treatment
- T-cell and Retrovirus Studies
- Virology and Viral Diseases
- interferon and immune responses
- Herpesvirus Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- Respiratory viral infections research
- Bacterial Infections and Vaccines
- Biosensors and Analytical Detection
- Zoonotic diseases and public health
- Parasites and Host Interactions
- Mosquito-borne diseases and control
- Traumatic Brain Injury and Neurovascular Disturbances
- Machine Learning and ELM
- Adrenal and Paraganglionic Tumors
Nationwide Children's Hospital
2017-2024
Baylor College of Medicine
2021-2023
Texas Children's Hospital
2023
Academy of Scientific and Innovative Research
2022
Central Electronics Engineering Research Institute
2022
Government of Uttar Pradesh
2021
University of South Africa
2021
Defence Institute of Advanced Technology
2021
East Asia School of Theology
2021
Indian Institute of Technology Jammu
2021
The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the main target for neutralizing antibodies (NAbs). S trimer anchored in virion membrane its prefusion (preS) but metastable form. preS has been stabilized by introducing two or six proline substitutions, to generate stabilized, soluble 2P HexaPro (6P) proteins. Currently, it not known which form most immunogenic. Here, we generated recombinant vesicular stomatitis virus (rVSV) expressing preS-2P,...
The lack of a relevant, tractable, and immunocompetent animal model for hepatitis C virus (HCV) has severely impeded investigations viral persistence, immunity, pathogenesis. In the absence models with robust HCV infection, homolog hepaciviruses in their natural host could potentially provide useful surrogate models. We isolated rodent hepacivirus from wild rats (Rattus norvegicus), RHV-rn1; acquired complete genome sequence; developed an infectious reverse genetics system. RHV-rn1 resembles...
Abstract Efforts to develop an effective vaccine against the hepatitis C virus (HCV; human hepacivirus) have been stymied by a lack of small animal models. Here, we describe experimental rat model chronic HCV-related hepacivirus infection and its response T cell immunization. Immune-competent rats challenged with rodent (RHV) viremia characterized expansion non-functional CD8 + cells. Single-dose vaccination recombinant adenovirus vector expressing non-structural proteins induces immunity in...
With the rapid increase in SARS-CoV-2 cases children, a safe and effective vaccine for this population is urgently needed. The MMR (measles/mumps/rubella) has been one of safest most human vaccines used infants children since 1960s. Here, we developed live attenuated recombinant mumps virus (rMuV)–based candidates using MuV Jeryl Lynn (JL2) strain backbone. soluble prefusion spike protein (preS) gene, stablized by two prolines (preS-2P) or six (preS-6P), was inserted into genome at P–M F–SH...
Identifying viruses in synanthropic animals is necessary for understanding the origin of many that can infect humans and developing strategies to prevent new zoonotic infections. The white-footed mouse, Peromyscus leucopus, one most abundant rodent species northeastern United States. We characterized serum virome 978 free-ranging P. leucopus mice caught Pennsylvania. identified belonging 26 different virus families. Among these was a highly divergent segmented flavivirus whose genetic...
The current pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) has led to dramatic economic and health burdens. Although the worldwide SARS-CoV-2 vaccination campaign begun, exploration other vaccine candidates is needed due uncertainties with approved vaccines, such as durability protection, cross-protection against variant strains, costs long-term production storage. In this study, we developed a methyltransferase-defective...
Pegiviruses frequently cause persistent infection (as defined by >6 months), but unlike most other Flaviviridae members, no apparent clinical disease. Human pegivirus (HPgV, previously GBV-C) is detectable in 1–4% of healthy individuals and another 5–13% are seropositive. Some evidence for bone marrow spleen exists. Equine 1 (EPgV-1) not linked to disease, whereas pegivirus, Theiler's disease-associated virus (TDAV), was identified an outbreak acute serum hepatitis (Theiler's disease)...
Equine hepacivirus (EqHV) is phylogenetically the closest relative of HCV and shares genome organization, hepatotropism, transient or persistent infection outcome, ability to cause hepatitis. Thus, EqHV studies are important understand equine liver disease further as an outbred surrogate animal model for pathogenesis protective immune responses. Here, we aimed characterize course associated responses.Seven horses were experimentally inoculated with EqHV, monitored 6 months, rechallenged same...
C57BL/6 mice immunized intradermally (i.d.) with bacillus Calmette Guerin (BCG) plus killed skin-stage schistosomula are protected against subsequent infection Schistosoma mansoni, whereas immunization by i.v. or i.m. routes is not protective. Moreover, previous via the nonprotective route interfered ability to subsequently induce protection i.d. vaccination, suggesting that inhibitory responses invoked. Given evidence activated macrophages (M phi) play a role as effector cells in...
Background: Metagenomic next-generation sequencing offers an unbiased approach to identifying viral pathogens in cerebrospinal fluid of patients with meningoencephalitis unknown etiology. Methods: In 11-month case series, we investigated the use metagenomic diagnose infections among pediatric hospitalized presenting encephalitis or Cerebrospinal from known enterovirus meningitis were included as positive controls. primary intracranial hypertension serve controls without infections. Results:...
Immune correlates of hepatitis C virus (HCV) clearance and control remain poorly defined due to the lack an informative animal model. We recently described acute chronic rodent HCV-like (RHV) infections in lab mice. Here, we developed MHC class I II tetramers characterize serial changes RHV-specific CD8 CD4 T cells during infection C57BL/6J RHV induced rapid expansion targeting viral structural nonstructural proteins. After clearance, virus-specific transitioned from effectors long-lived...
Viruses capable of causing persistent infection have developed sophisticated mechanisms for evading host immunity, and understanding these processes can reveal novel features the immune system. One such virus, human pegivirus (HPgV), infects ~15% global population, but little is known about its biology beyond fact that it does not cause overt disease. We passaged a isolate feral brown rats (RPgV) in immunodeficient laboratory mice to develop mouse-adapted virus (maPgV) established high-titer...
Measles virus (MeV) has been an excellent vector platform for delivering vaccines against many pathogens because of its high safety and efficacy, induction long-lived immunity. Early in the COVID-19 pandemic, a recombinant MeV (rMeV) expressing prefusion full-length spike protein stabilized by two prolines (TMV-083) was developed tested phase 1 1/2 clinical trials but discontinued insufficient immunogenicity low seroconversion rate adults. Here, we compared rMeV soluble (preS) (rMeV-preS-2P)...
Background: Astroblastoma is a rare primary brain tumor of unclear origin, often occurring in young patients less than 30-years-old. It typically arises supratentorially and diagnosed based on histological features including vascular hyalinization perivascular pseudorosettes. Recent molecular characterization CNS high-grade neuroepithelial tumors with meningioma I alteration (HGNET- MN1 ) found that HGNET- morphological signatures astroblastoma clustered together. Further analysis revealed...
Nine members of the family Herpesviridae infect equines and two them (EHV1 EHV4) are globally significant pathogens causing respiratory disease, abortion more rarely paralysis.The ability equid herpesviruses to establish life-long latent infection in lymphoid neural tissues with periodic reactivation shedding is central maintenance these viruses horse populations.Over 50% horses become latently infected after EHV1 EHV4.During latency, expression viral genes highly restricted few or no...
Resection of brain lesions associated with refractory epilepsy to achieve seizure control is well accepted. However, concurrent behavioral effects these such as changes in mood, personality, and cognition the surgery on behavior have not been characterized. We describe 5 children epileptogenic significant abnormalities which improved after surgery.Five (ages 3-14 years) major lesional were identified treated at our center. Behavioral problems included academic impairment, impulsivity,...
Concern about elevated disease risk following disasters has been growing with the progression of global trends in urbanization and climate, part because shifts socioecological conditions can promote greater human contact pathogen reservoirs cities. Remarkably little is known, however, diversity distributions pathogens carried by commensal across disaster-affected urban landscapes. To address this deficit, we characterized assemblage structure viruses serum three widespread rodents (Rattus...
Comparative study between HI and Indirect ELISA antibody titres in samples for NDV Virus poultry was carried out at Deptt. of Veterinary Microbiology, LUVAS, Hisar. As we all know that Newcastle disease is also known as Ranikhet India. causes a worldwide birds e.g. chickens, turkeys, guinea fowl, pheasants pigeons. The principal aim this to compare with tests the diagnosis virus (NDV) infections causing respiratory problems poultry. However, comparative has been shown test reproducible have...
Astrocytomas are the most common primary brain tumor with prognosis highly dependent on Grading of tumor. With limited tissue available in stereotactic biopsy and often not representing area concern, development new antibodies targeted against specific proteins mitotically active cells is necessary. Ki67/MIB-1 LI has conventionally been used along mitotic count to grade astrocytomas. However Ki67 limitation that it expressed by through cell cycle difficult differentiate from apoptotic cells....