A5102953035- Escherichia coli research studies
- Toxoplasma gondii Research Studies
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Autophagy in Disease and Therapy
- Viral gastroenteritis research and epidemiology
- Urinary Tract Infections Management
- Cell Adhesion Molecules Research
- Vibrio bacteria research studies
- Pediatric Urology and Nephrology Studies
- Bacterial Genetics and Biotechnology
- Adenosine and Purinergic Signaling
- Antibiotic Resistance in Bacteria
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Cannabis and Cannabinoid Research
- Tuberculosis Research and Epidemiology
- Mechanisms of cancer metastasis
- Virology and Viral Diseases
- Immune cells in cancer
- Inflammasome and immune disorders
- RNA modifications and cancer
- Heme Oxygenase-1 and Carbon Monoxide
- Infectious Disease Case Reports and Treatments
- Sirtuins and Resveratrol in Medicine
Nankai University
2019-2025
Ningxia Medical University General Hospital
2025
Ningxia Medical University
2025
Tianjin Economic-Technological Development Area
2019-2024
Beijing Chest Hospital
2024
Capital Medical University
2022-2024
Ministry of Education of the People's Republic of China
2024
Huazhong Agricultural University
2020-2023
Center of Hubei Cooperative Innovation for Emissions Trading System
2020-2023
Chinese Academy of Agricultural Sciences
2019-2022
Uropathogenic Escherichia coli (UPEC) is a major cause of urinary tract infections (UTIs). Invasion into bladder epithelial cells (BECs) on the luminal surface via type 1 fimbria first critical step in UPEC infection. Although expression increases during invasion BECs, underlying regulatory mechanisms remain poorly understood. This study reported previously uncharacterized two-component system (TCS) GrpP/GrpQ that directly activates to promote and therefore pathogenicity response D-serine...
Urinary tract infections are predominantly caused by uropathogenic Escherichia coli (UPEC). UPEC infects bladder epithelial cells (BECs) via fusiform vesicles, escapes into the cytosol to evade exocytosis, and establishes intracellular bacterial communities (IBCs) for next round of infection. The vesicle escape mechanism remains unclear. Here we show that senses host immune responses initiates upregulating a key phospholipase. phospholipase PldA disrupts membrane, pldA expression is...
In apicomplexan parasites, the macroautophagy/autophagy machinery is repurposed to maintain plastid-like organelle apicoplast. Previously, we showed that in Toxoplasma and Plasmodium, ATG12 interacts with ATG5 a non-covalent manner, contrast covalent interaction most organisms. However, it remained unknown whether parasites have functional orthologs of ATG16L1, protein essential for function ATG12–ATG5 complex vivo other Furthermore, mechanism used by autophagy apicoplast unclear. We report...
Alzheimer's disease is the most common dementia characterized by chronic progressive neurodegeneration. The incidence of on rise as population ages at an accelerating pace. According to epidemiological data, 2050, number patients in United States will be three times higher than that 2010, and a similar trend occurring China. To explore effect mechanism let-7b detecting expression level disease, fifty with thirty healthy controls were selected. levels let-7 families (let-7a, let-7b, let-7c,...
Abstract Background Targeting the TGF-β pathway in tumor therapy has proven challenging due to highly context-dependent functions of TGF-β. Integrin αvβ8, a pivotal activator TGF-β, been implicated signaling within tumors, as demonstrated by significant anti-tumor effects anti-αvβ8 antibodies. Nevertheless, expression profile αvβ8 remains subject debate, and precise mechanisms underlying antibodies are not yet fully elucidated. Methods We utilized single-cell RNA sequencing assess across...
CXCR7 (ACKR3) has been well-supported as a promoter of growth and metastasis in hepatocellular carcinoma (HCC). Both Hippo signaling play roles organ development. We aimed to verify the involvement Hippo-YAP CXCR7-regulated HCC proliferation, migration, invasion. HCCLM3 cells were transfected with si-CXCR7, pcDNA-CXCR7, or related control RNA/empty vector. Cell proliferation was assessed using Counting Kit-8 (CCK-8), mRNA protein levels measured via quantitative real-time PCR (qPCR) Western...
Triple negative breast cancer (TNBC) is an exceptionally aggressive subtype of with a poor prognosis. TNBC patients have limited treatment options beyond conventional chemotherapy, and they face significant challenges associated disease recurrence resistance to chemotherapy. The phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/mechanistic target rapamycin (mTOR) signaling pathway plays pivotal role in cell proliferation, growth, metabolism, survival. Its aberrant activation closely...
Many viral proteins are related to suppressing apoptosis in target cells and hence beneficial replication. The V protein of Newcastle disease virus (NDV) is one such that plays an important role inhibiting a species-specific manner. However, date, there have been no reports clarifying the antiapoptotic mechanisms protein. present study was undertaken determine apoptotic potential chicken embryo fibroblast cell line (DF-1 cell) elucidate its molecular action. Here, yeast two-hybrid system...
The porcine reproductive and respiratory syndrome virus (PRRSV) remains a persistent hazard in the global pig industry. DEAD (Glu-Asp-Ala-Glu) box helicase 21 (DDX21) is member of DDX family. In addition to its function regulating cellular RNA metabolism, DDX21 also regulates innate immunity involved replication cycle some viruses. However, relationship between PRRSV has not yet been explored. Here, we found that overexpression promoted replication, whereas knockdown reduced proliferation....
Porcine reproductive and respiratory syndrome virus (PRRSV) has devastated the swine industry worldwide for over 30 years shows no signs of slowing down. In this study, we found that PRRSV infection elevated hypoxia-inducible factor 1α (HIF-1α) expression.
Adherent-invasive
Background Adherent–invasive E. coli (AIEC) LF82 is capable of adhering to and invading intestinal epithelial cells, as well replicating within macrophages without inducing host cell death. Methods We compared the transcriptomics at pH=7.5 pH=5.8 by RNA-sequencing, qRT-PCR verified differentially expressed genes (DEGs). The deletion mutants DEGs in treatment group (pH=5.8) control (pH=7.5) were constructed λ recombinant. replication differences between WT infected Raw 264.7 24 h.p.i analyzed...
Urinary tract infections are primarily caused by uropathogenic Escherichia coli (UPEC). UPEC infects bladder epithelial cells (BECs) via fusiform vesicles and escapes into the cytosol disrupting vesicle membrane using outer phospholipase PldA, establishes biofilm-like intracellular bacterial communities (IBCs) for protection from host immune clearance. Cytosolic is captured autophagy to form autophagosomes, then transported lysosomes, triggering spontaneous exocytosis of lysosomes. The...
Abstract Deoxyribonucleic acid (DNA) methylation plays a key role in gene regulation and is critical for development human disease. Techniques such as whole-genome bisulfite sequencing (WGBS) reduced representation (RRBS) allow DNA analysis at the genome scale, with Illumina NovaSeq 6000 MGI Tech DNBSEQ-T7 being popular due to their efficiency affordability. However, detailed comparative studies of performance are not available. In this study, we constructed 60 WGBS RRBS libraries two...
The lifecycle of avian leukosis virus subgroup J (ALV-J), a typical tumorigenic retrovirus, is highly dependent upon host cellular proteins. However, there have been few studies directed at uncovering the proteins responsible for ALV-J replication, which could provide insights into new strategies prevention and control. Here, we used proteomics to identify association differential levels collagen triple helix-repeat-containing 1 (CTHRC1) with viral replication. Our results revealed that...
Uropathogenic Escherichia coli (UPEC) is the most common causative agent of urinary tract infection (UTI). UPEC invades bladder epithelial cells (BECs) via fusiform vesicles, escapes into cytosol, and establishes biofilm-like intracellular bacterial communities (IBCs). Nucleoside-diphosphate kinase (NDK) secreted by pathogenic bacteria to enhance virulence. However, whether NDK involved in pathogenesis remains unclear. Here, we find that lack ndk impairs colonization CFT073 mouse bladders...
Escherichia coli K1 is the leading cause of neonatal gram-negative bacterial meningitis, but pathogenesis E meningitis remains unclear. Blood-brain barrier (BBB) penetration a crucial step in development. Here, we uncovered role CsiR, GntR family regulator, virulence. During infection, csiR expression was induced due to derepression by Fur blood and human brain microvascular endothelial cells (HBMECs). CsiR positively regulated ilvB expression, which associated with branched chain amino acid...
Costimulatory and co-inhibitory receptors on T lymphocytes play an essential role in the immune response. There is increasing evidence that expression of co-signal molecules cells altered infection, tumor, autoimmunity, other diseases, intervention can be used immunotherapy. This paper reviewed costimulatory coinhibitory