A5067162010- Malaria Research and Control
- Mosquito-borne diseases and control
- Parasites and Host Interactions
- Viral Infections and Outbreaks Research
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- COVID-19 epidemiological studies
- Global Maternal and Child Health
- Hepatitis B Virus Studies
- Chronic Disease Management Strategies
- vaccines and immunoinformatics approaches
- MicroRNA in disease regulation
- Hepatitis C virus research
- Viral Infections and Vectors
- Influenza Virus Research Studies
- Dialysis and Renal Disease Management
- Hepatitis Viruses Studies and Epidemiology
- Chronic Kidney Disease and Diabetes
- Advanced biosensing and bioanalysis techniques
- Extracellular vesicles in disease
- Vaccine Coverage and Hesitancy
- RNA modifications and cancer
- Diabetes, Cardiovascular Risks, and Lipoproteins
- COVID-19 diagnosis using AI
- Global Health and Epidemiology
Ifakara Health Institute
2011-2025
An ongoing phase 3 study of the efficacy, safety, and immunogenicity candidate malaria vaccine RTS,S/AS01 is being conducted in seven African countries.From March 2009 through January 2011, we enrolled 15,460 children two age categories--6 to 12 weeks 5 17 months age--for vaccination with either or a non-malaria comparator vaccine. The primary end point analysis was efficacy against clinical during after first 6000 at enrollment who received all three doses according protocol. After 250 had...
Several studies showed that assessing levels of specific circulating microRNAs (miRNAs) is a non-invasive, rapid, and accurate method for diagnosing diseases or detecting alterations in physiological conditions. We aimed to identify serum miRNA signature be used the diagnosis tuberculosis (TB). To account variations due genetic makeup, we enrolled adults from two study settings Europe Africa. The following categories subjects were considered: healthy (H), active pulmonary TB (PTB), TB, HIV...
The RTS,S/AS01(E) malaria candidate vaccine is being developed for immunization of African infants through the Expanded Program Immunization (EPI).This phase 2, randomized, open, controlled trial conducted in Ghana, Tanzania, and Gabon evaluated safety immunogenicity when coadministered with EPI vaccines. Five hundred eleven were randomized to receive at 0, 1, 2 months (in 3 doses diphtheria, tetanus, whole-cell pertussis conjugate [DTPw]; hepatitis B [HepB]; Haemophilus influenzae type b...
In sub-Saharan Africa (SSA), epidemiological data for chronic kidney disease (CKD) are scarce. We conducted a prospective cross-sectional study including 952 patients in an outpatient clinic Tanzania to explore CKD prevalence estimates and the association with cardiovascular infectious disorders. According KDIGO, we measured albumin-to-creatinine ratio calculated eGFR using CKD-EPI formula. Factors associated were by logistic regression. Venn diagrams modelled visualize interaction between...
The RTS,S/AS malaria candidate vaccine is being developed with the intent to be delivered, if approved, through Expanded Programme on Immunization (EPI) of World Health Organization. Safety, immunogenicity and efficacy RTS,S/AS02D when integrated into a standard EPI schedule for infants have been reported over nine-month surveillance period. This paper describes results following 20 months follow up. Phase IIb, single-centre, randomized controlled trial enrolled 340 in Tanzania receive three...
The malaria vaccine RTS,S induces antibodies against the Plasmodium falciparum circumsporozoite protein (CSP) and concentration of Immunoglobulin G (IgG) repeat region CSP following vaccination is associated with protection from P. malaria. So far, only quantity anti-CSP IgG has been measured used to predict success, although quality (measured as avidity) antigen-antibody interaction shall be important since a few sporozoites circulate for short time after an infectious mosquito bite, likely...
Malaria parasite infections that are only detectable by molecular methods highly prevalent and represent a potential transmission reservoir. The used to detect these not standardized, their operating characteristics often unknown. We designed proficiency panel of Plasmodium spp. in order compare the accuracy detection protocols labs clinical trial consortium. Ten dried blood spots (DBSs) were assembled contained P. falciparum, vivax, malariae, ovale; DBSs either single species or mixed with...
Abstract Genetic analysis of molecular markers is critical in tracking the emergence and/or spread artemisinin resistant parasites. Clinical isolates collected western Kenya pre- and post- introduction combination therapies (ACTs) were genotyped at SNP positions regions strong selection signatures on chromosome 13 14, as described Southeast Asia (SEA). Twenty five SNPs using Sequenom MassArray pfmdr 1 gene copy number by real-time PCR. Parasite clearance half-life vitro drug sensitivity...
A critical impediment to efficient outbreak response is the availability of timely and complete data on cases their linkage care. To inform solutions this issue, study investigated utility self-testers reporting coronavirus disease 2019 results using a mobile health platform. Our has demonstrated that platform feasible; it achieved 74.5% rate, indicating strong likelihood entry into unstructured supplementary service Support from community workers (CHWs) healthcare professionals,...
Although recent reports on congenital malaria suggest that the incidence is increasing, it difficult to determine whether clinical disease due parasites acquired before delivery or as a result of contamination by maternal blood at birth. Understanding method parasite acquisition important for estimating time and design preventive measures. The aim this study was first Plasmodium falciparum in infants same present placenta Babies born mothers with P. detected PCR were followed up two years...
Summary Objectives This enquiry aimed to provide a snap‐shot of availability, price and quality malaria rapid diagnostic tests (RDTs) in private health facilities at selected sites six malaria‐endemic countries Africa, South East Asia America. Methods In each study site, data collectors surveyed healthcare which were based on accessibility from their home institution. Using questionnaire, information was recorded about the facility itself RDT(s) available. Where possible, small number RDTs...
Malaria rapid diagnostic tests (mRDTs) have played an important role in the early detection of clinical malaria endemic area. While several mRDTs are currently on market, availability with high sensitivity and specificity will merit fight against malaria. We evaluated field performance a novel One Step (P.f/P.v) Tri-line (P.f) test kits Pwani, Tanzania.In cross-sectional study conducted Bagamoyo Kibiti districts Tanzania, symptomatic patients were tested using SD BIOLINE, kits, microscope,...
Abstract The use of malaria rapid diagnostic tests (RDTs) as a source for nucleic acids that can be analyzed via acid amplification techniques has several advantages, including minimal amounts blood, sample collection, simplified storage and shipping conditions at room temperature. We have systematically developed extensively evaluated procedure to extract total from used RDTs. co-extraction DNA RNA molecules small volumes dried blood retained on the RDTs allows detection quantification P....
Innovative approaches are needed to limit antimalarial resistance evolution. Understanding the role of intermittent preventive treatment in pregnancy (IPTp) on selection for and impact such has outcomes can guide future interventions.Plasmodium falciparum isolates (n = 914) from 2 randomized clinical trials were screened pfmdr1 copy number variation pfcrt, pfmdr1, pfdhfr, pfdhps markers. The conducted between 2010 2013 Benin, Gabon, Kenya, Mozambique establish efficacy IPTp-mefloquine (MQ)...
Introduction Epidemiological data about diabetes mellitus (DM) for sub-Saharan Africa (SSA) are scarce and the utility of glycated hemoglobin (HbA1c) to diagnose DM is uncertain in African populations with a high proportion anemia. Research design methods In cross-sectional study, age-adjusted prevalence rates predictors pre-DM were prospectively assessed by HbA1c semirural walk-in population Tanzania (n=992). Predictors calculated logistic regression. Correlations between HbA1c, hemoglobin,...
Although studies on COVID-19 vaccine hesitancy are being undertaken widely worldwide, there is limited evidence in Tanzania. This study aims to assess the sociodemographic factors associated with and reasons given by unvaccinated participants. We conducted a mixed-method cross-sectional two components-health facilities communities-between March September 2022. A structured questionnaire in-depth interviews were used collect quantitative qualitative data, respectively. total of 1,508...
Bagamoyo Research and Training Centre (BRTC), a branch of the Ifakara Health Institute (IHI), established in late 2004, has evolved into leading site performing high-quality Phase II III malaria vaccine drug trials according to ICH/GCP standards. Several assessments interventions focused on better diagnosis, treatment prevention have been completed successfully. Expansion areas TB, with set up new BSL-3, HIV/AIDS marks commitment developing regional centre excellence for both clinical...
Abstract Background Malaria is endemic in Tanzania with majority of clinical cases caused by Plasmodium falciparum . Additionally, malariae and ovale spp. are also present manifestations these infections not well described. Clinical episodes P. often characterized a relatively mild illness low number parasites, which can persist for long periods. In this report, two that were identified during trial evaluating the malaria vaccine candidate, PfSPZ Vaccine The participants followed up...
Abstract The use of malaria rapid diagnostic tests (RDTs) as a source for nucleic acids that can be analyzed via acid amplification techniques has several advantages, including minimal amounts blood, sample collection, simplified storage and shipping conditions at room temperature. We have systematically developed extensively evaluated procedure to extract total from used RDTs. co-extraction DNA RNA molecules small volumes dried blood retained on the RDTs allows detection quantification P....
During the COVID-19 pandemic, need for making testing readily available was recognized as an important factor individuals to help make informed decisions, including isolate or seek care, and policymakers control transmission. Toward this end, FIND Access Tools Accelerator funded 16 rapid operational research studies one implementation project in Africa, Caribbean, Asia evaluating utility, acceptability, feasibility of different community-based SARS-CoV-2 approaches. Here, we discuss common...
Abstract A pilot implementation of the rapid diagnostic test program was performed to collect evidence feasibility, acceptability, and uptake COVID-19 AgRDT in Tanzania. We conducted a prospective cross-sectional study community provide quantitative details antigen (AgRDT) This undertaken between March 2022 September 2022. The implemented by distributing offering kits people suspected having Dar es Salaam through health workers. total 1039 participants consented participate survey. All...
The rapid diagnosis of coronavirus disease 2019 (COVID-19) is critical for comprehensive public health response strategies, and self-testing with antigen diagnostic tests (Ag-RDTs) presents opportunities to test in hard-to-reach communities. Therefore, we evaluated the acceptability, feasibility, uptake Ag-RDT at community level Tanzania. From June October 2022, symptomatic individuals or those recent contact a known suspected COVID-19 patient were offered assisted testing within mining...