A5101472553- Lymphoma Diagnosis and Treatment
- CAR-T cell therapy research
- DNA Repair Mechanisms
- Chronic Lymphocytic Leukemia Research
- Protein Degradation and Inhibitors
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Microtubule and mitosis dynamics
- Cancer Genomics and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Marine Sponges and Natural Products
- Cancer-related Molecular Pathways
- Plant biochemistry and biosynthesis
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Cutaneous lymphoproliferative disorders research
- Cancer-related gene regulation
- Acute Myeloid Leukemia Research
- Synthetic Organic Chemistry Methods
- Cancer-related molecular mechanisms research
- Lipid metabolism and biosynthesis
- Vascular Malformations Diagnosis and Treatment
- Advanced Synthetic Organic Chemistry
- Bladder and Urothelial Cancer Treatments
- Phytase and its Applications
University of Newcastle Australia
2022-2025
Dana-Farber Cancer Institute
2003-2024
Harvard University
2022-2024
Hunter Medical Research Institute
2023
Boston University
2023
First Affiliated Hospital of Guangzhou Medical University
2023
Guangzhou Medical University
2023
Peking University
2017-2021
Jiangsu Vocational College of Medicine
2021
Second Xiangya Hospital of Central South University
2021
The cellular pathways that restart stalled replication forks are essential for genome stability and tumor prevention. However, how many of these exist in cells selectively activated remain unclear. Here, we describe two major fork pathways, demonstrate their selection is governed by 53BP1 BRCA1, which known to control the pathway choice repair double-strand DNA breaks (DSBs). Specifically, promotes a cleavage-free pathway, whereas BRCA1 facilitates break-induced (BIR) coupled with SLX-MUS...
Abstract P53 inactivation occurs in about 50% of human cancers, where p53‐driven p21 activity is devoid and p27 becomes essential for the establishment G1/S checkpoint upon DNA damage. Here, this work shows that E2F1‐responsive lncRNA LIMp27 selectively represses expression contributes to proliferation, tumorigenicity, treatment resistance p53‐defective colon adenocarcinoma (COAD) cells. competes with mRNA binding cytoplasmically localized hnRNA0, which otherwise stabilizes leading cell...
Cycloartenol synthase converts oxidosqualene to cycloartenol, a pentacyclic isomer of the animal and fungal sterol precursor lanosterol. We used directed evolution find cycloartenol residues that affect cyclopropyl ring formation, selecting randomly generated mutants for their ability genetically complement yeast strain lacking lanosterol synthase. To increase likelihood finding novel mutations, little-studied Dictyostelium discoideum was mutagenesis. Several catalytically important were identified.
Rationale: Despite landmark therapy of chronic myelogenous leukemia (CML) with tyrosine kinase inhibitors (TKIs), drug resistance remains problematic. Cancer pathogenesis involves epigenetic dysregulation and in particular, histone lysine demethylases (KDMs) have been implicated TKI resistance. We sought to identify KDMs altered expression CML define their contribution imatinib Methods: Bioinformatics screening compared KDM versus normal bone marrow shRNA knockdown flow cytometry used...
The MRE11-RAD50-NBS1 (MRN) complex is well known for participating in DNA damage response pathways all phases of cell cycle. Here, we show that MRN constitutes a mitosis-specific complex, named mMRN, with protein, MMAP. MMAP directly interacts MRE11 and required optimal stability the during mitosis. colocalizes mitotic spindles, MMAP-deficient cells display abnormal spindle dynamics chromosome segregation similar to MRN-deficient cells. Mechanistically, both are hyperphosphorylated by...
Gene fusions involving tumor protein p63 gene (TP63) occur in multiple T and B cell lymphomas portend a dismal prognosis for patients. The function mechanisms of TP63 remain unclear, there is no target therapy patients with lymphoma harboring fusions. Here, we show that act as bona fide oncogenes are essential fusion-positive lymphomas. Transgenic mice expressing TBL1XR1::TP63, the most common fusion, develop diverse recapitulate human identify coordinate recruitment two epigenetic modifying...
[reaction: see text] The dammarenyl cation (13) is the last common intermediate in cyclization of oxidosqualene to a diverse array secondary triterpene metabolites plants. We studied structure and reactivity 13 understand factors governing regio- stereospecificity synthesis. First, we demonstrated that has 17beta side chain Arabidopsis thaliana lupeol synthase (LUP1) by incubating substrate analogue (18E)-22,23-dihydro-20-oxaoxidosqualene (21) with LUP1 from recombinant yeast strain devoid...
Gossypium hirsutum and G. barbadense mitochondrial genomes were analyzed to understand the factors shaping codon usage. While most analyses of usage suggest minimal no bias, nucleotide composition, specifically GC content, was significantly correlated with In general, both favor codons that end in A or U, a secondary preference for pyrimidine rich codons. These observations are similar previous reports cotton nuclear genomes, possibly suggestive general bias spanning genomic compartment....
To study the chemical constituents of Periploca forrestii.The were separate using such various column chromatographic techniques as silica gel, RP-18 MCI and Sephadex LH-20. Their structures identified by methods spectral analysis.Ten compounds isolated periforgenin A-3-O-beta-digitoxopyranoside (1), beta-sitosterol (2), periforoside I (3), ursolic acid (4), periplogenin (5), periplocin (6), glycoside E (7), periplocoside M (8) , daucosterol (9), 2alpha, 3alpha,...
Endoplasmic reticulum stress (ERS) has sizeable affect on cancer proliferation, metastasis, immunotherapy and chemoradiotherapy resistance. However, the effect of ERS biochemical recurrence (BCR) prostate patients remains elusive. Here, we generated an ERS-related genes risk signature to evaluate physiological function in PCa with BCR.We collected from GeneCards. The edgeR package was used screen differential TCGA datasets. gene then established using LASSO multivariate Cox regression models...
Abstract Phosphoinositide 3-kinase gamma (PI3Kγ) is implicated as a target to repolarize tumor-associated macrophages and promote anti-tumor immune responses in solid cancers. However, cancer cell-intrinsic roles of PI3Kγ are unclear. Here, by integrating unbiased genome-wide CRISPR interference screening with functional analyses across acute leukemias, we define selective dependency on the complex high-risk subset that includes myeloid, lymphoid, dendritic lineages. This characterized...