A5101803501- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Lung Cancer Treatments and Mutations
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Tryptophan and brain disorders
- Colorectal Cancer Treatments and Studies
- Gastric Cancer Management and Outcomes
- Cancer Immunotherapy and Biomarkers
- Genetic factors in colorectal cancer
- Cancer, Lipids, and Metabolism
- Diet and metabolism studies
- Chemokine receptors and signaling
- Sperm and Testicular Function
- Pancreatic and Hepatic Oncology Research
- Cancer Mechanisms and Therapy
- Peptidase Inhibition and Analysis
- Genomics and Phylogenetic Studies
- Cancer-related molecular mechanisms research
- Thermoregulation and physiological responses
- Synthesis and Catalytic Reactions
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Reproductive biology and impacts on aquatic species
Fudan University Shanghai Cancer Center
2021-2025
Shanghai Medical College of Fudan University
2024-2025
Southwest Medical University
2024
Peking University Shenzhen Hospital
2024
Ruijin Hospital
2018-2022
Shanghai Jiao Tong University
2018-2022
Central South University
2008-2020
Xiangya Hospital Central South University
2020
The First Affiliated Hospital, Sun Yat-sen University
2019
Sun Yat-sen University
2015-2019
Gastric cancer (GC) has a clear predilection for metastasis toward the omentum which is primarily composed of adipose tissue, indicating that fatty acids may contribute to this phenomenon. However their function remains poorly understood in GC. In study, we investigated role palmitate acid (PA) and its cellular receptor CD36 progression GC.Immunohistochemical (IHC) staining was performed detect expression GC tissues clinical significance determined statistically. over-expression knock-down...
IDO1 (Indoleamine 2,3-dioxygenase 1) inhibits host anti-tumor immune response by exhausting tryptophan in tumor microenvironment, but the pathogenic mechanisms of gastric cancer (GC) cells need to be further explored.The aim this study was use CCLE (Cancer Cell Line Encyclopedia) transcriptomic data GC cell lines for WGCNA (Weighted Gene Co-expression Network Analysis) analysis, and explore potential functions progression vitro vivo.The higher expression level identified 4 out 7 lines....
Abstract Purpose: C-X-C chemokine receptor type 2 (CXCR2) is a key regulator that drives immune suppression and inflammation in tumor microenvironment. CXCR2-targeted therapy has shown promising results several solid tumors. However, the underlying mechanism of CXCR2-mediated cross-talk between gastric cancer cells macrophages still remains unclear. Experimental Design: The expression CXCR2 its ligands 155 human tissues was analyzed via immunohistochemistry, correlations with clinical...
COVID-19 is a public health emergency that has rapidly spread to over 200 countries and regions, no effective treatment been established date. Severe critical cases have associated with higher mortality due acute respiratory distress syndrome (ARDS) cytokine storm. Based on the novelty recent emergence of COVID-19, regimen identified, thus prompting clinicians engage in drug repurposing address immediate therapeutic need. This study focused molecular target angiotensin-converting enzyme 2...
Abstract T cell exhaustion plays critical roles in tumor immune evasion. Novel strategies to suppress evasion are urgent need. We aimed identify potential compounds target and increase response checkpoint inhibitors (ICIs). Differentially expressed genes (DEGs) were identified between tumors with different by comparing the transcriptome data. DEGs then analyzed Connectivity Map (CMap) platform ICIs. Gene set enrichment analysis, LDH release assay, Chromatin immunoprecipitation (ChIP), Co-IP...
Purinergic mechanisms appear to be involved in motor as well sensory functions the urinary bladder. ATP released from efferent nerves excites bladder smooth muscle, whereas urothelial cells can activate afferent and cells. In present study, we used immunohistochemical techniques examine distribution of purinoceptors urothelium, normal cat possible changes expression these receptors cats with a chronic painful condition termed feline interstitial cystitis (FIC) which release urothelium is...
The human reference genome is still incomplete, especially for those population-specific or individual-specific regions, which may have important functions. Here, we developed a HUman Pan-genome ANalysis (HUPAN) system to build the pan-genome. We applied it 185 deep sequencing and 90 assembled Han Chinese genomes detected 29.5 Mb novel genomic sequences at least 188 protein-coding genes missing in (GRCh38). It can be an resource genome-related biomedical studies, such as cancer analysis....
This study explored potential biomarkers associated with Lauren classification of gastric cancer. We screened microarray datasets on cancer information in GEO database, and compared differentially expressing genes between intestinal-type or diffuse-type Four sets data (GSE2669, GSE2680, GDS3438 GDS4007) were enrolled into analysis. By differential gene analysis, UBE2C, CDH1, CENPF, ERO1L, SCD, SOX9, CKS1B, SPP1, MMP11 ANLN identified as the top related to cancer, MGP, FXYD1, FAT4, SIPA1L2,...
Globozoospermia is a severe form of teratozoospermia characterized by round-headed spermatozoa with an absent acrosome, aberrant nuclear membrane and midpiece defects.Globozoospermia diagnosed the presence 100% on semen analysis, patients this condition are absolutely infertile.The objective study was to investigate differences in protein expression between human roundheaded normal spermatozoa.Two-dimensional (2-D) fluorescence difference gel electrophoresis (DIGE) coupled mass spectrometry...
Gambogic acid is a natural component isolated from gamboge that possesses anticancer properties. Our previous study suggested gambogic might be involved in autophagy; however, its role pancreatic cancer remained unclear.Cell viability and apoptosis of cell lines were determined using (4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan flow cytometry. The effects on autophagy was assessed by western blot, acridine orange staining, transmission electron microscopy, measurement autophagic flux...
Abstract Background Accumulative evidence shows that an organoid is a more practical and reliable tool in cancer biology research. This study aimed to identify validate crucial genes involved non-small cell lung carcinogenesis development using the transcriptomic analysis of tumor tissues organoids. Methods Gene set enrichment (GSEA) tissues, organoids, normal was performed reveal similar different mechanisms adenocarcinoma (LUAD) squamous carcinoma (LUSC) progression. Differentially...
Abstract Pangenomic study might improve the completeness of human reference genome (GRCh38) and promote precision medicine. Here, we use an automated pipeline pangenomic analysis to build gastric cancer pan-genome for 185 paired deep sequencing data (370 samples), characterize gene presence-absence variations (PAVs) at whole level. Genes ACOT1 , GSTM1, SIGLEC14 UGT2B17 are identified as highly absent genes in population. A set from unaligned sequences with GRCh38 predicted. We successfully...
Lapatinib is a small molecule inhibitor of EGFR (HER1) and ERBB2 (HER2) receptors, which used for treatment advanced or metastatic breast cancer. To find the drug resistance mechanisms EGFR/ERBB2 positive tumors, we analyzed possible effects lncRNAs. In this study, using CCLE (Cancer Cell Line Encyclopedia) database, explored relationship between lncRNAs sensitivity/resistance, then validated those findings through in vitro experiments. We found that expression activation ERBB pathway was...
Gastric cancer (GC) continues to result in a poor survival rate and prognostic biomarkers for the disease are lacking. Chemokine (C-X-C motif) ligand (CXCL1) expression plays critical role tumor metastasis, Snail promotes epithelial-mesenchymal transition (EMT) promote metastasis. Therefore, present study aimed investigate correlation between CXCL1 effect of on patients with GC. paraffin-embedded tissue sections from 127 GC were each assessed by immunohistochemistry. Cox regression...
Exploring ERBB2-related pathways will help us finding sensitive molecules and potential combined therapeutic targets of ERBB2-targeted therapy for ERBB2+ gastric cancer (GC). In this study, we performed a cross-databases study focused on GC. The data GC deposited in the genome atlas (TCGA), gene expression omnibus (GEO), InBio MapTM, cell line encyclopedia (CCLE), therapeutics response portal (CTRP) were analyzed. correlation levels candidate IC50 genes-targeted drugs verified NCI-N87 MKN-45...
Objective: Recently, immune checkpoints blockers showed higher anti-tumor activity for advanced gastric cancer (GC). The purpose of the study is to find out predictive biomarkers related anti-cytotoxic lymphocyte antigen 4 (CTLA4) therapy.Materials and methods: Datasets gene expression omnibus (GEO), genome atlas (TCGA), set enrichment analysis (GESA) were extracted. Differential CTLA4 between tissues normal mucosa, WT (wild type) vs. CTLA4_KO (knockout) upregulated clinical significance...
Chemotherapy resistance is the leading cause of cancer treatment failure. This research was conducted to explore a potential link between actin-binding protein anillin (ANLN) and doxorubicin in breast cancer.We compared ANLN expression 50% inhibition concentration (IC50) human cells (MDA-MB-231) with (MDA-MB-231/ADM). Co-immunoprecipitation used investigate interaction RhoA. The cell viability, apoptosis, gene were estimated by MTT, flow cytometry, quantitative real-time PCR western blot.The...
Abstract Lack of guidelines for personalized chemotherapy treatment after surgery has caused gastric cancer (GC) patients' unnecessary exposure to toxicity and the financial burden treatments. In our study, we aimed identify potential biomarkers predict GC susceptibility platinum‐based on Gene Expression Omnibus (GEO) data sets. A total 603 differentially expressed genes (DEGs) were identified between platinum‐resistant cell lines platinum‐sensitive based Cancer Cell Line Encyclopedia (CCLE)...
IPO-38 is a potential biomarker for early diagnosis of gastric cancer that we recently identified. Although characterized its chemical nature as nucleosome histone, suspected the existence histone modification antibody-labeled protein. Here, used commercially available modified peptide array to identify type and site labeled by monoclonal antibody. In protein analysis, citrulline 3 on arginine 26 (H3R26Cit) yielded strongest signal. peptidyl deiminase-2 -4 (PADI2 PADI4, respectively) can...