A5100377424- Cell Adhesion Molecules Research
- Hippo pathway signaling and YAP/TAZ
- Cellular Mechanics and Interactions
- Ubiquitin and proteasome pathways
- Ion channel regulation and function
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Plant Pathogens and Fungal Diseases
- Cancer, Hypoxia, and Metabolism
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Cancer Cells and Metastasis
- Cellular transport and secretion
- Photosynthetic Processes and Mechanisms
- Glycosylation and Glycoproteins Research
- Circular RNAs in diseases
- Retinoids in leukemia and cellular processes
- FOXO transcription factor regulation
- 3D Printing in Biomedical Research
- Caveolin-1 and cellular processes
- Mechanisms of cancer metastasis
- Autophagy in Disease and Therapy
- Signaling Pathways in Disease
- Microtubule and mitosis dynamics
- Endoplasmic Reticulum Stress and Disease
Chinese Academy of Medical Sciences & Peking Union Medical College
2024-2025
Ruijin Hospital
2025
Shanghai Jiao Tong University
2025
Henan University
2019-2025
First Affiliated Hospital of Henan University
2025
Xi'an Jiaotong University
2024
Northeast Agricultural University
2024
Yunnan University
2024
Sichuan University
2023
Jilin University
2023
Tumor‐associated macrophages have important roles in hepatocellular carcinoma (HCC) initiation and progression. Long noncoding RNAs (lncRNAs) also been reported to be involved HCC. In this study, we explored how lncRNA LINC00662 may influence HCC progression through both tumor cell‐dependent macrophage‐dependent mechanisms. was found upregulated HCC, high levels correlated with poor survival of patients. WNT3A expression secretion via competitively binding miR‐15a, miR‐16, miR‐107. Through...
Abstract Cell metabolism is strongly influenced by mechano-environment. We show here that a fraction of kindlin-2 localizes to mitochondria and interacts with pyrroline-5-carboxylate reductase 1 (PYCR1), key enzyme for proline synthesis. Extracellular matrix (ECM) stiffening promotes translocation into its interaction PYCR1, resulting in elevation PYCR1 level consequent increase synthesis cell proliferation. Depletion reduces level, increases reactive oxygen species (ROS) production...
Precise control of mesenchymal stem cell (MSC) differentiation is critical for tissue development and regeneration. We show here that kindlin-2 a key determinant MSC fate decision. Depletion in MSCs sufficient to induce adipogenesis inhibit osteogenesis vitro vivo. Mechanistically, regulates through controlling YAP1/TAZ at both the transcript protein levels. Kindlin-2 physically associates with myosin light-chain kinase response mechanical cues microenvironment intracellular signaling events...
Reprograming of proline metabolism is critical for tumor growth. Here we show that PINCH-1 highly expressed in lung adenocarcinoma and promotes synthesis through regulation mitochondrial dynamics. Knockout (KO) increases dynamin-related protein 1 (DRP1) expression fragmentation, which suppresses kindlin-2 translocation interaction with pyrroline-5-carboxylate reductase (PYCR1), resulting inhibition cell proliferation. Depletion DRP1 reverses deficiency-induced defects on dynamics,...
The promyelocytic leukemia protein (PML) is essential in the assembly of dynamic subnuclear structures called PML nuclear bodies (PML-NBs), which are involved regulating diverse cellular functions. However, possibility being cardiac disease has not been examined. In mice undergoing transverse aortic constriction (TAC) and arsenic trioxide (ATO) injection, transforming growth factor β1 (TGF-β1) was upregulated along with alteration SUMOylation. cultured neonatal mouse fibroblasts (NMCFs),...
Mitochondrial damage-associated molecular patterns (DAMPs) including mitochondrial DNA (mtDNA), TFAM (transcription factor A, mitochondrial), and ATP, which play crucial roles in the regulation of inflammatory environment human diseases. However, role DAMPs regulating tumor microenvironment (TME) remains unclear. Herein, we demonstrate that infiltration M2-type tumor-associated macrophages (TAMs) was correlated with resistance hepatocellular carcinoma (HCC) to sorafenib. We found cell-free...
Abstract Pancreatic stellate cells (PSCs) contribute to pancreatic ductal adenocarcinoma (PDAC) progression and therapeutic resistance, yet their detailed functions remain unclear. This study combined RNA sequencing assay for transposase‐accessible chromatin using (ATAC‐seq) on sorted PSCs from adjacent normal PDAC tissues investigate transcriptional epigenetic activation. heterogeneity are characterized through bulk, single‐cell, spatial transcriptomes, as well in situ sequencing. The...
Abstract TEFM (transcription elongation factor of mitochondria) has been identified as a novel nuclear-encoded transcription in the mitochondrial genome. Our bioinformatics analysis TCGA data revealed an aberrant over-expression hepatocellular carcinoma (HCC). We analyzed its biological effects and clinical significance this malignancy. expression was by quantitative real-time PCR, western blot, immunohistochemistry HCC tissues cell lines. The on growth metastasis were determined...
Abstract Background Lung cancer remains a major cause of cancer-related mortality throughout the world at present. Repositioning existing drugs for other diseases is promising strategy therapies, which may rapidly advance potentially agents into clinical trials and cut down cost drug development. Ciclopirox (CPX), an iron chelator commonly used to treat fungal infections, has recently been shown have antitumor activity against variety cancers including both solid tumors hematological...
Pulmonary fibrosis is a progressive and fatal lung disease characterized by activation of fibroblasts excessive deposition collagen matrix. We show here that the concentrations kindlin-2 its binding partner PYCR1, key enzyme for proline synthesis, are significantly increased in tissues human patients with pulmonary fibrosis. Treatment TGF-β1 markedly expression resulting mitochondrial translocation, formation kindlin-2–PYCR1 complex, synthesis. The complex synthesis were reduced response to...
Patient-derived tumor organoids (PDTOs) shows great potential as a preclinical model. However, the current methods for establishing PDTOs primarily focus on modulating local properties, such sub-micrometer topographies. Nevertheless, they neglect to capture global millimeter or intermediate mesoscale architecture that have been demonstrated influence response therapeutic treatment and progression. In this study, we present rapid technique generating collagen bundles with an average length of...
Mechano-environment plays multiple critical roles in the control of mesenchymal stem cell (MSC) fate decision, but underlying signaling mechanisms remain undefined. We report here a axis consisting PINCH-1, SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), and bone morphogenetic type 2 receptor (BMPR2) that links mechano-environment to MSC decision. PINCH-1 interacts with Smurf1, which inhibits latter from interacting BMPR2 consequently suppresses degradation, resulting augmented BMP...
In animal cells, the dysregulation of centrosome duplication and cohesion maintenance leads to abnormal spindle assembly chromosomal instability, contributing developmental disorders tumorigenesis. However, molecular mechanisms involved in maintaining accurate number control tethering are not fully understood. Here, we identified coiled-coil domain-containing 102A (CCDC102A) as a centrosomal protein exhibiting barrel-like structure proximal regions parent centrioles, where it prevents...
Abstract A new compound xylarkarynone ( 1 ), a first reported natural product B 2 ) and eight known compounds 3 ‐ 10 were isolated from Xylaria sp. HHY‐2. Their structures elucidated by spectroscopic methods, DP4+ probability analyses electronic circular dichroism (ECD) calculations. The bioactivities of assayed. Compound exhibited obvious activity against A549 cells with an IC 50 value 6.12±0.28 μM. Additionally, showed moderate antifungal activities Plectosphaerella cucumerina Aspergillus...
Pin1-directed prolyl isomerization is a central common oncogenic mechanism to drive tumorigenic processes. However, the role of Pin1 in cellular autophagy still poorly understood. Here we report that pharmacological inhibition decreased formation autophagosome/autolysosomes upon nutrient starvation. Inhibition reduced, whereas forced expression increased, level LC3 and viability U2OS PANC-1 cells. could augment accumulation chloroquine treatment, while also disturbed its function on cell...