A5018744650- Parasitic Diseases Research and Treatment
- Insects and Parasite Interactions
- SARS-CoV-2 detection and testing
- Mosquito-borne diseases and control
- Parasites and Host Interactions
- Biosensors and Analytical Detection
- Malaria Research and Control
- Parasite Biology and Host Interactions
- SARS-CoV-2 and COVID-19 Research
- Advanced Biosensing Techniques and Applications
- Insect symbiosis and bacterial influences
- Advanced biosensing and bioanalysis techniques
- Vector-borne infectious diseases
- Respiratory viral infections research
- Vibrio bacteria research studies
- Dental Health and Care Utilization
- Nutrition and Health in Aging
- Immunodeficiency and Autoimmune Disorders
- Acute Myeloid Leukemia Research
- Global Maternal and Child Health
- COVID-19 diagnosis using AI
- Complement system in diseases
- Child Nutrition and Water Access
- Fecal contamination and water quality
- Dysphagia Assessment and Management
Program for Appropriate Technology in Health
2016-2025
Seattle University
2022-2025
Westlake Health Center
2022
Rutgers Sexual and Reproductive Health and Rights
2020
Rutgers, The State University of New Jersey
2020
University of Washington
2010-2012
MicroVision (United States)
2010
Micron (United States)
2010
Institute for Systems Biology
2002
Onchocerciasis is a neglected tropical disease caused by infection with the parasite Onchocerca volvulus (Ov). An estimated 180 million people are at risk for Ov infection, and 37 infected, mostly in Africa. A lateral flow-based assay to detect human IgG4 antibodies Ov-specific antigen Ov-16 was developed as rapid tool exposure Ov. The test, when performed on 449 sera specimens from patients microfiladermia Ov-negative patients, has sensitivity of 89.1% (95% confidence interval:...
Elimination programs for Wuchereria bancrofti and Onchocerca volvulus are in critical need of sensitive, specific, point-of-contact (POC) tools that can be used surveillance years beyond cessation mass drug administration when infection intensities low. Previously, Wb123 Ov16 were identified individually as potential filarial antigens an antibody-based POC test. The present study compares single-antigen Wb123- Ov16-based tests with integrated configuration to detect antibodies...
ABSTRACT The Global Programme to Eliminate Lymphatic Filariasis has an urgent need for rapid assays detect ongoing transmission of lymphatic filariasis (LF) following multiple rounds mass drug administration (MDA). Current WHO guidelines support using the antigen card immunochromatographic test (ICT), which detects active filarial infection but does not early exposure LF. Recent studies found that antibody-based better serve this function. In present study, two tests, a IgG4 enzyme-linked...
Diagnostics provide a means to measure progress toward disease elimination. Many countries in Africa are approaching elimination of onchocerciasis after successful implementation mass drug administration programs as well vector control. An understanding how markers for infection such skin snip microfilaria and Onchocerca volvulus-specific seroconversion perform near-elimination settings informs best use these markers. All-age participants from 35 villages Togo were surveyed 2013 2014...
Abstract Background: Rapid diagnostic tests (RDTs) used to diagnose Plasmodium falciparum (Pf) predominantly target the antigen Histidine Rich Protein 2 (HRP2) exclusively. With emergence of hrp2/hrp3 gene deletions, RDTs targeting other antigens such as essential enzyme Lactate Dehydrogenase (LDH) are needed. The dynamics LDH relative HRP2 currently not well described but needed inform use next-generation (NG-) and that designed address deletions. Methods: A longitudinal cohort study...
Abstract Background Rapid diagnostic tests (RDTs) used to diagnose Plasmodium falciparum predominantly target the antigen Histidine Rich Protein 2 (HRP2) exclusively. With emergence of hrp2/hrp3 gene deletions, RDTs targeting other antigens such as essential enzyme Lactate Dehydrogenase (LDH) are needed. The dynamics LDH relative HRP2 currently not well described but needed inform use next-generation (NG-) and that designed address deletions. Methods A longitudinal cohort study conducted in...
Background Onchocerciasis is targeted for elimination in Africa through annual or biannual ivermectin mass drug administration (MDA). An immunodiagnostic test, based on the detection of human IgG4 antibodies blood to Onchocerca volvulus-specific antigen Ov16, one recommended tools determining whether transmission interrupted and treatment can stop. For different settings, relationship between post-MDA Ov16 antibody prevalence children (measured 1 year after last round MDA) duration coverage...
We report a simple fluidic system that can purify and concentrate diagnostic biomarkers through the capture triggered release of stimuli-responsive polymer−antibody conjugates at porous membranes are grafted with same polymer. This technique is applied here to detection model streptavidin antigen subsequently clinical ranges malaria Plasmodium falciparum histidine-rich protein 2 (PfHRP2) from spiked human plasma. The carboxyl end-groups semi-telechelic poly(N-isopropylacrylamide) (pNIPAAm)...
As effective onchocerciasis control efforts in Africa transition to elimination efforts, different diagnostic tools are required support country programs. Senegal, with its long standing, successful program, is transitioning using the SD BIOLINE Onchocerciasis IgG4 (Ov16) rapid test over traditional skin snip microscopy. The aim of this study demonstrate feasibility integrating Ov16 into surveillance activities based on following attributes acceptability, usability, and cost. A...
Serological assessments for human onchocerciasis are based on IgG4 reactivity against the OV-16 antigen, with sensitivities of 60-80%. We have previously identified 7 novel proteins that could improve serodiagnosis.IgG4 responses to these were assessed by luciferase immunoprecipitation (LIPS) and enzyme-linked immunosorbent (ELISA) immunoassays.OVOC10469 OVOC3261 as most promising candidates IgG4-based immunoassays 53% rOVOC10469 78% rOVOC3261 while specificity each was >99%. These 2...
Background Serological assays for human IgG4 to the Onchocerca volvulus antigen Ov16 have been used confirm elimination of onchocerciasis in much Americas and parts Africa. A standardized source positive control antibody (human anti-Ov16 IgG4) will ensure quality surveillance data using these tests. Methodology/Principal Findings recombinant was identified by screening against a synthetic Fab phage display library converted into IgG4. This developed different formulations enzyme-linked...
The World Health Organization currently recommends assessing elimination of onchocerciasis by testing whether Ov16 antibody prevalence in children aged 0-9 years is below 0.1%. However, the certainty evidence for this recommendation considered to be low. We used established ONCHOSIM model investigate predictive value different Ov16-antibody thresholds various age groups a variety endemic settings and mass drug administration scenarios. According our simulations, depends highly on precontrol...
The diagnosis of onchocerciasis in endemic areas has been demanding given the need to replace invasive skin snip method with a more sensitive and specific rapid point-of-contact tool. Filarial antigen detection tests are better alternative methods diagnosing Onchocercal infections, as they detect infections could be used monitor transmission following mass drug administration. With shift paradigme from control elimination, point- of-contact tool is required support elimination programs. This...
ABSTRACT. Laboratory benchmarking allows objective analysis of the analytical performance malaria rapid diagnostic tests (RDTs). We present detection limits Rapigen BIOCREDIT Malaria Ag Pf/Pv (pLDH/pLDH), Pf (pLDH/HRPII), and two best-in-class WHO-prequalified comparator RDTs, generated using standardized panels containing recombinant antigen, in vitro cultured parasites, international standards, clinical samples. Detection limit antigen concentrations HRP2, PfLDH, PvLDH were determined for...
Malaria antigen detection through rapid diagnostic tests (RDTs) is widely used to diagnose malaria and estimate prevalence. To support more sensitive next-generation RDT development screen asymptomatic malaria, we developed evaluated the Q-Plex™ Human Array (Quansys Biosciences, Logan, UT), which quantifies antigens commonly in RDTs—Plasmodium falciparum–specific histidine-rich protein 2 (HRP2), P. falciparum-specific lactate dehydrogenase (Pf LDH), Plasmodium vivax–specific LDH (Pv Pan (Pan...
This study assesses and compares the performance of different swab types specimen collection sites for SARS-CoV-2 testing, to reference standard real-time reverse transcriptase-polymerase chain reaction (RT-PCR) viral culture.Symptomatic adults with COVID-19 who visited routine testing used spun polyester FLOQSwabs self-collect specimens from anterior nares tongue. We evaluated self-collected tongue swabs nucleocapsid (N) or spike (S) antigen by RT-PCR then compared these results cultures...
Abstract Background The recent expansion of tools designed to accurately quantify malaria parasite-produced antigens has enabled us evaluate the performance rapid diagnostic tests (RDTs) as a function they detect—typically histidine rich protein 2 (HRP2) or lactate dehydrogenase (LDH). Methods For this analysis, whole blood specimens from longitudinal study in Bancoumana, Mali were used ultra-sensitive HRP2-based Alere™ Malaria Ag P.f RDT (uRDT). samples collected part transmission-blocking...
The SD Bioline® IgG4 rapid diagnostic test (RDT) detects antibodies induced by the Onchocerca volvulus-specific antigen Ov16. We evaluated stability of RDT results over 1 month, at different time points after completion each assay, using eluted dried blood spots collected in central Cameroon. Agreement coefficients regarding positivity between 30 minutes and 24 hours, 1, 2, 3, 4 weeks were, 96.4%, 93.4%, 93.3%, 93.2%, respectively. Between 3.6% 15,444 tests showed inconsistent with 81.2%...
Severe acute respiratory coronavirus-2 (SARS-CoV-2) is a novel viral pathogen and therefore challenge to accurately diagnose infection. Asymptomatic cases are common so it difficult identify infected support surveillance case detection. Diagnostic test developers working meet the global demand for accurate rapid diagnostic tests disease management. However, focus of many these has been on molecular tests, more recently serologic use in primarily high-income countries. Low- middle-income...
Rapid diagnostic tests (RDTs) that detect antigen indicative of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection can help in making quick health care decisions and regularly monitoring groups at risk infection. With many RDT products entering the market, it is important to rapidly evaluate their relative performance. Comparison clinical evaluation study results challenged by protocol design variations populations. Laboratory assays were developed quantify nucleocapsid...
The relationship between N-antigen concentration and viral load within across different specimens guides the clinical performance of rapid diagnostic tests (RDT) in uses. A prospective study was conducted Porto Velho, Brazil, to investigate RDT specimen types as a function correlation antigen load. included 214 close contacts with recent exposures confirmed cases, aged 12 years older various levels vaccination. Antigen measured nasopharyngeal swab (NPS), anterior nares (ANS), saliva...