A5004027294- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Retinal Development and Disorders
- Corneal surgery and disorders
- Corneal Surgery and Treatments
- Retinal Imaging and Analysis
- Retinal and Optic Conditions
- Ocular Surface and Contact Lens
- Mesenchymal stem cell research
- Ocular Diseases and Behçet’s Syndrome
- Nerve injury and regeneration
- Ophthalmology and Visual Impairment Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinopathy of Prematurity Studies
- Adipose Tissue and Metabolism
- Cerebrovascular and genetic disorders
- Neurogenesis and neuroplasticity mechanisms
- Systemic Lupus Erythematosus Research
- Retinoids in leukemia and cellular processes
- Photoreceptor and optogenetics research
- Tissue Engineering and Regenerative Medicine
- Neurological Disease Mechanisms and Treatments
- Pluripotent Stem Cells Research
- Tea Polyphenols and Effects
- Advanced Glycation End Products research
Chinese University of Hong Kong
2016-2025
Shantou University
2018-2025
University of Hong Kong
2000-2025
Shantou University Medical College
2018-2024
Creative Commons
2022
University of Pittsburgh
2018-2022
Geriatric Research Education and Clinical Center
2012-2018
Hong Kong Eye Hospital
2014-2018
King's College London
2015
University of Miami
2013-2014
Abstract Retinal fundus diseases can lead to irreversible visual impairment without timely diagnoses and appropriate treatments. Single disease-based deep learning algorithms had been developed for the detection of diabetic retinopathy, age-related macular degeneration, glaucoma. Here, we a platform (DLP) capable detecting multiple common referable conditions (39 classes) by using 249,620 images marked with 275,543 labels from heterogenous sources. Our DLP achieved frequency-weighted average...
To determine the expressions of SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) and type II transmembrane serine protease (TMPRSS2) genes in human mouse ocular cells comparison to other tissue cells. Human conjunctiva primary pterygium tissues were collected from patients who underwent surgery. The expression ACE2 TMPRSS2 was determined conjunctival cells, cell lines, mesenchymal stem as well by reverse transcription-polymerase chain reaction (RT-PCR) SYBR green PCR. RT-PCR...
Cigarette smoking contributes to the development of destructive periodontal diseases and delays its healing process. Our previous study demonstrated that nicotine, a major constituent in cigarette smoke, inhibits regenerative potentials human ligament-derived stem cells (PDLSC) through microRNA (miRNA) regulation. In this study, we hypothesized delayed smokers is caused by afflicted potential smoker PDLSC. We cultured PDLSC from teeth extracted non-smokers. PDLSC, found significantly reduced...
Diabetic retinopathy (DR) is a leading cause of blindness that poses significant public health concerns worldwide. Increasing evidence suggests neuroinflammation plays key role in the early stages DR. Microglia, long-lived immune cells central nervous system, can become activated response to pathological insults and contribute retinal neuroinflammation. However, molecular mechanisms microglial activation during DR are not fully understood. In this study, we used vivo vitro assays investigate...
Abstract Optic neuropathies are the leading cause of irreversible blindness and visual impairment in developed countries, affecting more than 80 million people worldwide. While most optic have no effective treatment, there is intensive research on retinal ganglion cell (RGC) protection axon regeneration. We previously demonstrated potential human periodontal ligament-derived stem cells (PDLSCs) for replacement. Here, we report neuroprotective effect PDLSCs to ameliorate RGC degeneration...
<h3>Importance</h3> A retinopathy of prematurity (ROP) diagnosis currently relies on indirect ophthalmoscopy assessed by experienced ophthalmologists. deep learning algorithm based retinal images may facilitate early detection and timely treatment ROP to improve visual outcomes. <h3>Objective</h3> To develop a image–based, multidimensional, automated, platform for screening validate its performance accuracy. <h3>Design, Setting, Participants</h3> total 14 108 eyes 8652 preterm infants who...
To identify the risk factors for development of diabetic retinopathy (DR), macular edema (DME), and sight threatening DR (STDR) based on a city-wide diabetes screening program. Diabetic patients were prospectively recruited between June 2016 December 2022. All underwent dilated fundus photography centered disc macula or spectral domain optical coherence tomography (SD-OCT) scan. Complete medical history was documented.Systematic examination, blood analysis, urinalysis performed. Multivariate...
purpose. Variants in the complement factor H (CFH) gene have been shown to be strongly associated with age-related macular degeneration (AMD). In this study, sequence alterations CFH were investigated 163 Chinese patients exudative AMD and 155 unrelated control subjects. methods. All 22 exons, intron–exon boundaries, promoter sequences screened by polymerase chain reaction DNA sequencing. results. Fifty-eight changes, 42 of them novel, identified. Six SNPs an allele frequency >30%...
Mapping the genes for age-related macular degeneration (AMD) had not been successful until recent genome-wide association studies revealed Tyr402His in CFH and rs11200638 HTRA1 as AMD-related genetic variants. This study was conducted to identify other critical factors that are associated with exudative AMD.The promoter, splice regions, coding exons of were sequenced 163 patients AMD 183 sex- age-matched control subjects. Also documented genotype smoking status.Four significant SNPs found...
Background Epigenetic factors, such as microRNAs, are important regulators in the self-renewal and differentiation of stem cells progenies. Here we investigated microRNAs expressed human limbal-peripheral corneal (LPC) epithelia containing epithelial progenitor (CEPCs) early transit amplifying cells, their role epithelium. Methodology/Principal Findings Human LPC was extracted for small RNAs or dissociated CEPC culture. By Agilent microRNA Microarray V2 platform GeneSpring GX11.0 analysis,...
Adult stem cells are critical for the healing process in regenerative medicine. However, cigarette smoking inhibits cell recruitment to tissues and delays wound-healing process. This study investigated effect of nicotine, a major constituent smoke, on potentials human mesenchymal (MSC) periodontal ligament-derived (PDLSC). The proliferation 1.0 μM nicotine-treated MSC PDLSC was significantly reduced when compared untreated control. Moreover, nicotine also retarded locomotion these adult...
Abstract Green tea extracts exhibit anti-oxidative and anti-inflammatory actions in different disease conditions. We hypothesized that green extract its catechin constituents ameliorate sodium iodate-induced retinal degeneration rats by counteracting oxidative stress. In this study, adult Sprague-Dawley were intravenously injected with a single dose of iodate. (GTE; Theaphenon-E) or combinations constituents, including (−)-epigallocatechin gallate (EGCG), administered intra-gastrically...
Abstract Retinal diseases are the leading causes of irreversible visual impairment and blindness in developed countries. Human retina has limited regenerative power to replace cell loss. Stem replacement therapy been proposed as a viable option. Previously, we have induced human adult periodontal ligament stem cells (PDLSCs) retinal lineage. In this study, modified our induction protocol direct PDLSCs into ganglion-like determined microRNA (miRNA) signature transdifferentiation process. The...
Abstract Angiopoietin 2 (ANG2) is a proangiogenic cytokine which may have an implication in neovascular age related macular degeneration (nAMD). In 24 eyes of subjects presenting with treatment naïve nAMD and 26 control patients, aqueous humor samples were collected at the time intervention (intravitreal injection anti-vascular endothelial growth factor or cataract extraction). Best corrected visual acuity (BCVA) central thickness (CMT) using optical coherence tomography (OCT) measured...
To investigate the utility of metagenomic next-generation sequencing (mNGS) in identifying pathogens endophthalmitis.In this prospective study, 36 cases endophthalmitis were recruited. All patients received surgical treatment and intraocular drug lavage. The samples vitreous or aqueous humor extracted for mNGS microbiological culture. diagnostic performance was compared between culture.The positive rates culture 88.89% (32/36) 27.78% (10/36), respectively. There a statistically significant...
To investigate the plasma lipoprotein subclasses in patients with primary open-angle glaucoma (POAG), a total of 20 Chinese POAG on intraocular pressure (IOP)-lowering treatment and age-matched control subjects were recruited. Based levels cholesterol (TC) low-density (LDL-C), study divided into elevated- normal-level subgroups. The lipoprotein, subclasses, oxidized LDL (oxLDL) quantitatively measured. discrimination potential lipoproteins was evaluated using area under receiver operating...
To evaluate changes in intraocular pressure (IOP) dominant and contralateral eyes following the dark room prone provocative test (DRPPT) study subjects with shallow anterior chamber. This was a prospective, single-center, non-randomized controlled trial of 43 (86 eyes) The eye identified using card-hole method. underwent DRPPT for one hour. Pre- post-DRPPT IOP measured an I-Care tonometer, pupillary parameters, including amplitude constriction, peak constriction velocity, re-dilation were...
purpose. To evaluate the genotypic and phenotypic correlations of Bietti's crystalline dystrophy (BCD) in patients with CYP4V2 gene by mutation screening clinical electrophysiological assessment. methods. Eighteen Chinese 13 families BCD were recruited for full ophthalmic examinations, optical coherence tomography (OCT), visual tests, including electrooculography (EOG), full-field electroretinography (ERG), multifocal (mfERG). Peripheral venous blood was obtained from all index their family...