A5069176028- Total Knee Arthroplasty Outcomes
- Orthopaedic implants and arthroplasty
- Knee injuries and reconstruction techniques
- Foot and Ankle Surgery
- Orthopedic Infections and Treatments
- Bone Metabolism and Diseases
- Rheumatoid Arthritis Research and Therapies
- Tendon Structure and Treatment
- Bone health and osteoporosis research
- Orthopedic Surgery and Rehabilitation
- RNA Interference and Gene Delivery
- Bone health and treatments
- NF-κB Signaling Pathways
- Wound Healing and Treatments
- Osteoarthritis Treatment and Mechanisms
- Chronic Lymphocytic Leukemia Research
- Virus-based gene therapy research
- Bone and Joint Diseases
- Cytokine Signaling Pathways and Interactions
- interferon and immune responses
- Psoriasis: Treatment and Pathogenesis
- Spondyloarthritis Studies and Treatments
- Cardiac Fibrosis and Remodeling
- Autoimmune and Inflammatory Disorders Research
- Surgical Sutures and Adhesives
Hoshigaoka Medical Center
2016-2025
Japan Community Healthcare Organization
2016-2021
Osaka University
2004-2015
Gene Therapy Laboratory
2006-2008
Recent clinical studies suggest that several antihypertensive drugs, especially angiotensin-converting enzyme inhibitors, reduced bone fractures. To clarify the relationship between hypertension and osteoporosis, we focused on role of angiotensin II (Ang II) metabolism. In marrow-derived mononuclear cells, Ang (1x10(-6) M) significantly increased tartrate-resistant acid phosphatase (TRAP) -positive multinuclear osteoclasts. Of importance, induced expression receptor activator NF-kappaB...
Osteoarthritis (OA) is one of the most common joint diseases in elderly people, however, underlying mechanism OA pathogenesis not completely clear. Periostin, extracellular protein, has been shown by cDNA array analysis to be highly expressed OA, but its function fully understood. The purpose this study was examine expression and periostin human OA.Human cartilage synovia samples were used for function. obtained from knees patients undergoing total knee arthroplasty as femoral bone head with...
Abstract Background Safety issues are of paramount importance in clinical human gene therapy. From this point view, it would be better to develop a novel non‐viral efficient transfer method. Recently, was reported that ultrasound exposure could induce cell membrane permeabilization and enhance expression. Methods In study, we examined the potential for into kidney. First, transfected rat left kidney with luciferase plasmid mixed microbubbles, Optison, optimize conditions (duration...
Objectives: The retention of the anti-rheumatic agent tocilizumab (TCZ) has not been well documented in patients with rheumatoid arthritis (RA). We conducted an observational study to compare TCZ and anti-tumour necrosis factor (TNF) drugs treatment RA.Method: reviewed continuation rates causes discontinuation biological agents (biologics) by assessing medical records RA who were administered biologics at our institute from September 1999 April 2012, using Osaka University Biologics for...
Objective In this study, we examine how advancements in novel antirheumatic drugs affect the clinicopathologic features of lymphoproliferative disorder (LPD) patients with rheumatoid arthritis (RA). Methods multicenter study across 53 hospitals Japan, characterized RA who developed LPDs and visited between January 1999 March 2021. The statistical tools used included Fisher's exact test, Mann‐Whitney U ‐test, log‐rank logistic regression analysis, Cox proportional hazards models. Results...
Hyaluronan (HA), one of the major structural extracellular components in cartilage, regulates cellular responses via receptors such as CD44. However, direct effects HA on osteoblastic differentiation has not been studied detail. Here, we investigated (molecular weight: 900–1200kDa) that was induced by bone morphogenetic protein (BMP) C2C12 cells (mouse myoblastic cells) and ST2 marrow cells). BMP‐induced Smad1/Smad5/Smad8 phosphorylation were downregulated HA. Use CD44‐blocking antibody...
Abstract Objective To examine the capacity of bone marrow CD34+ cells to generate endothelial cells, in order assess role neovascularization synovium rheumatoid arthritis (RA). Methods purified from 13 patients with active RA and 9 control subjects (7 osteoarthritis [OA] 2 healthy individuals) were cultured presence stem cell factor (10 ng/ml) granulocyte–macrophage colony‐stimulating (1 ng/ml). After 18 days incubation, generation was assessed by flow cytometry. The compared degree...
Objectives: To clarify the difference of patient-based outcome between joint-preserving arthroplasty and resection-replacement in forefoot surgery for patients with rheumatoid arthritis (RA). Methods: A total 63 feet 49 RA who underwent were asked to answer pre-operative post-operative self-administered foot evaluation questionnaire (SAFE-Q). Patients treated either (1) metatarsal head (28 feet, mean age 63.8 years, follow-up 4.2 DAS28-CRP 2.2) or (2) metatarsophalangeal (35 63.1 3.6 2.1) at...
Abstract Background For the development of molecular therapy based on oligodeoxynucleotides (ODN), ODN have to be stable against nucleases and specific target transcription factor. To decrease non‐specific binding degradation from 3′‐terminus ODN, we designed partially annealed by extremities two single strands, resulting in a ribbon‐shaped so called ribbon‐type decoy (R‐ODN). Methods We evaluated efficiency process enzymatic ligation R‐ODN, activity nuclear factor‐κB (NF‐κB), stability...
Agents to inhibit the renin-angiotensin system have been reported suppress progression of abdominal aortic aneurysm (AAA). However, effects calcium channel blockers (CCBs) are still unclear in terms inhibition AAA. Recently, several CCBs beyond those associated with blood pressure lowering attracted much interest. In this study, we examined nifedipine on AAA progression. was induced rats by transient perfusion elastase. Then, (10 mg/kg/day) and saline (control) were administered osmotic...
Objective: Hydrocellular foam dressings are characterised by their ability to create a moist wound healing environment and absorb high amounts of exudate. In this study, we examined the effect hydrocellular dressing on rat excisional wounds. Method: One full-thickness was made back rats wounds were treated with or gauze dressing. To examine histology wound, haematoxylin eosin (HE) staining performed. Gene expression vascular endothelial growth factor (VEGF), fibroblast (FGF), hepatocyte...
Abstract In this study we examined the effect of ribbon-type (circular-type) NF-κB decoy oligodeoxynucleotides (RNODN) on osteoclast induction and activity. We extracted bone marrow cells from femurs rats incubated non-adherent with receptor activator nuclear factor κB ligand (RANKL) macrophage colony-stimulating (M-CSF). First, transfer efficiency into osteoclasts their precursors, resistance to exonuclease, binding activity were examined. Next, examine RNODN activity, differentiation pit...
This study examined the ability of E2F decoy oligodeoxynucleotides (ODN) to inhibit proliferation synovial fibroblasts derived from patients with rheumatoid arthritis (RA). The effect ODN on cartilage invasion by RA synovium in a murine model human was also investigated. were introduced into tissue and using hemagglutinating virus Japan (HVJ)-liposomes. fibroblast evaluated MTT assay RT-PCR for cell cycle regulatory genes proliferating-cell nuclear antigen (PCNA) cyclin-dependent kinase 2...
Many clinical trials have demonstrated that angiotensin converting enzyme inhibitors protective effects on organ damage, suggesting the importance of inhibition renin-angiotensin system. In this study, we investigated a non-depressor dose imidapril damage induced by diabetes and hypertension. Diabetes was an intravenous injection streptozotocin (STZ, 40 mg/kg) in 15-week-old male spontaneously hypertensive rats (SHR). Imidapril (2 mg/kg/day) or vehicle given orally for 28 days, then heart...