A5084495617- Ubiquitin and proteasome pathways
- Click Chemistry and Applications
- Genomics and Chromatin Dynamics
- Chemical Synthesis and Analysis
- Epigenetics and DNA Methylation
- Sirtuins and Resveratrol in Medicine
- Protein Degradation and Inhibitors
- Plant Molecular Biology Research
- Antimicrobial Peptides and Activities
- Peptidase Inhibition and Analysis
- Histone Deacetylase Inhibitors Research
- CRISPR and Genetic Engineering
- Psoriasis: Treatment and Pathogenesis
- Phytochemistry and Bioactive Compounds
- Cancer-related gene regulation
- Mass Spectrometry Techniques and Applications
- Phytochemistry and biological activities of Ficus species
- Diabetes and associated disorders
- Biotin and Related Studies
- RNA Research and Splicing
- Mindfulness and Compassion Interventions
- Suicide and Self-Harm Studies
- PARP inhibition in cancer therapy
- Ferroptosis and cancer prognosis
- Plant Pathogenic Bacteria Studies
University of Hong Kong
2013-2024
Chinese University of Hong Kong
2020-2024
Hunan Normal University
2022
Nankai University
2012
Posttranslational modifications (PTMs) play a crucial role in wide range of biological processes. Lysine crotonylation (Kcr) is newly discovered histone PTM that enriched at active gene promoters and potential enhancers mammalian cell genomes. However, the cellular enzymes regulate addition removal Kcr are unknown, which has hindered further investigation its functions. Here we used chemical proteomics approach to comprehensively profile ‘eraser’ recognize lysine-4 crotonylated H3 (H3K4Cr)...
Methylation of histone H3 lysine-79 (H3K79) is an epigenetic mark for gene regulation in development, cellular differentiation, and disease progression. However, how this translated into downstream effects remains poorly understood owing to a lack knowledge about its readers. We developed nucleosome-based photoaffinity probe capture proteins that recognize H3K79 dimethylation (H3K79me2) nucleosomal context. In combination with quantitative proteomics approach, identified menin as H3K79me2...
Inflammatory bowel disease (IBD) has high prevalence in Western counties. The fat content diets is one of the leading causes for this prevalence; however, underlying mechanisms have not been fully defined. Here, we find that high-fat diet (HFD) induces ferroptosis intestinal regulatory T (Treg) cells, which might be key initiating step disruption immunotolerance and development colitis. Compared with effector Treg cells favor lipid metabolism prefer polyunsaturated fatty acids (PUFAs)...
Tag! You′re it! MalAM-yne is a chemical reporter for malonylation of lysines within proteins (see scheme), newly identified posttranslational modification. cell-permeable and metabolically incorporated into in living cells. Subsequent bioorthogonal tag conjugation allows the fluorescent visualization cellular profiling malonylated proteins.
Posttranslational modifications (PTMs) of lysine are crucial histone marks that regulate diverse biological processes. The functional roles and regulation mechanism many newly identified PTMs, however, remain yet to be understood. Here we report a photoaffinity crotonyl (Kcr) analogue can genetically site-specifically incorporated into proteins. This, in conjunction with the encoded photo-lysine as "control probe", enables capture identification enzymatic machinery and/or effector proteins...
Amino acid (aa) metabolism is closely correlated with the pathogenesis of psoriasis; however, details on aa transportation during this process are barely known. Here, we find that SLC38A5, a sodium-dependent neutral transporter counter-transports protons, markedly upregulated in psoriatic skin both human patients and mouse models. SLC38A5 deficiency significantly ameliorates psoriasis, indicating pathogenic role SLC38A5. Surprisingly, almost exclusively expressed dendritic cells (DCs) when...
Abstract IL-17+ γδ T cells (γδ T17) are kick-starters of inflammation due to their strict immunosurveillance xenobiotics or cellular damages and rapid response pro-inflammatory stimulators. IL-27 is a well-recognized pleiotropic immune regulator with potent inhibitory effects on type 17 responses. However, its actions T17 mediated the underlying mechanisms less well understood. Here we find that inhibits production IL-17 from cells. Mechanistically, promotes lipolysis while lipogenesis, thus...
Abstract Objective To analyze the effects of a novel compound, NK‐007, on prevention and treatment collagen‐induced arthritis (CIA) underlying mechanisms. Methods We determined effect NK‐007 lipopolysaccharide (LPS)–triggered tumor necrosis factor α (TNFα) production by murine splenocytes macrophage cell line (RAW 264.7) enzyme‐linked immunosorbent assay, intracellular cytokine staining, Western blotting. The LPS‐boosted CIA model was adopted, or vehicle administered at different time points...
Bromodomains, epigenetic "readers" of lysine acetylation marks, exist in different nuclear proteins with diverse biological functions chromatin biology. Malfunctions bromodomains are associated the pathogenesis human diseases, such as cancer. Bromodomains have therefore emerged therapeutic targets for drug discovery. Given high structural similarity bromodomains, a critical step development bromodomain inhibitors is evaluation their selectivity to avoid off-target effects. While numerous...
Multicellular organisms are composed of many tissue types that have distinct morphologies and functions, which largely driven by specialized proteomes interactomes. To define the proteome interactome a specific type in an intact animal, we developed localized proteomics approach called Methionine Analog-based Cell-Specific Proteomics Interactomics (MACSPI). This method uses tissue-specific expression engineered methionyl-tRNA synthetase to label proteins with bifunctional amino acid...
Chemical reporter, HMGAM-yne, facilitates the detection and identification of lysine HMGylation on histones.
Abstract Pericentromeric DNA, consisting of high-copy-number tandem repeats and transposable elements, is normally silenced through DNA methylation histone modifications to maintain chromosomal integrity stability. Although deacetylase 6 (HDA6) has been known participate in pericentromeric silencing, the mechanism still yet unclear. Here, using whole genome bisulfite sequencing (WGBS) chromatin immunoprecipitation-sequencing (ChIP-Seq), we mapped genome-wide patterns differential H3 lysine...
Markiert! MalAM-in ist ein chemischer Reporter für die Malonylierung von Lysinen in Proteinen (siehe Schema), eine neu identifizierte posttranslationale Modifikation. zellgängig und wird lebenden Zellen metabolisch Proteine eingebaut. Die anschließende Konjugation mit einem bioorthogonalen Marker ermöglicht Visualisierung der Malonierung Untersuchung malonierter mithilfe Fluoreszenz.
Histone chaperones are integral to chromatin dynamics, facilitating the assembly and disassembly of nucleosomes, thereby playing a crucial role in regulating gene expression maintaining genomic stability. Moreover, they prevent aberrant histone interactions prior assembly. Disruption chaperone function may result instability, which is implicated pathogenesis. This review aims elucidate cancer pathologies explore their potential as therapeutic targets. have been found be dysregulated various...