A5100657093- DNA Repair Mechanisms
- Telomeres, Telomerase, and Senescence
- Genetics and Neurodevelopmental Disorders
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Glioma Diagnosis and Treatment
- Mitochondrial Function and Pathology
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- Protein Degradation and Inhibitors
- Ferroptosis and cancer prognosis
- RNA Research and Splicing
- Pregnancy and preeclampsia studies
- Genomic variations and chromosomal abnormalities
- Chromosomal and Genetic Variations
- Chromatin Remodeling and Cancer
- Metabolomics and Mass Spectrometry Studies
- Genetic and Kidney Cyst Diseases
- Carcinogens and Genotoxicity Assessment
- Ginseng Biological Effects and Applications
- Phosphorus and nutrient management
- Synthetic Organic Chemistry Methods
- Renal and related cancers
- Advanced Biosensing Techniques and Applications
Guiyang Medical University
2021-2024
Central Hospital of Zibo
2024
Beijing Chao-Yang Hospital
2023
Capital Medical University
2023
Southwest Hospital
2020-2022
Army Medical University
2020-2022
Southwest General Health Center
2020
Kunming University of Science and Technology
2012-2019
Sichuan University
2019
Kunming University
2003-2018
Tumor mutational burden (TMB) is a potential biomarker for immune checkpoint therapy and prognosis. The impact of TMB on clinical outcomes the correlation coefficient between exome sequencing targeted in glioma have not yet been explored.Somatic mutations coding regions 897 primary gliomas RNA-seq data 654 patients Cancer Genome Atlas (TCGA) database were analyzed as training set, while another 286 Chinese Glioma (CGGA) used validation. Descriptive correlational analyses conducted with TMB....
We describe three families with X‐linked mental retardation, two a deletion of single amino acid and one missense mutation in the proximal domain RSK2(RPS6KA3) (ribosomal protein S6 kinase, 90 kDa, polypeptide 3) similar to mutations found Coffin–Lowry syndrome (CLS). In families, clinical diagnosis had been nonsyndromic retardation. third family, although CLS suspected, features were atypical degree intellectual disability much less than expected. These show that strict reliance on...
Abstract Autophagy is a highly conserved process that vital for tumor progression and treatment response. Although autophagy proposed to maintain the stemness phenotype in adult diffuse glioma, molecular basis of link between poorly understood, which makes it impossible effectively screen population will benefit from autophagy‐targeted treatment. Here, ATG9B as essential self‐renewal capacity tumor‐propagation potential identified. Notably, ASCL2 transcriptionally regulates expression...
The loss of wild type p53 tumor suppressive function and oncogenic gain-of-function mutants have been showing important implications in tumorigenesis.The N236S (p53 N239S human, p53S) mutation has shown to lose by yeast assay.However, its gain is still not clear.By gel shift assay, we showed that mutant p53S had lost DNA binding ability target promoters.Further real-time PCR data confirmed the regulating transcription p21 Cip1/Waf1 , cyclin G, PUMA, Bax response 10Gy irradiation.These...
In this study, nanocellulose (CNFs) was prepared by a mechanical shearing method, simple and pollution-free process. Iron hydroxide loaded on nanocellulose, natural macromolecule derived from bamboo, to produce the second-generation iron-loaded for removal of low-concentration phosphorus wastewater. We found that best modified ferric salt chloride. When mass ratio Fe(OH)3 CNFs 1.5 : 1, freeze-drying with liquid nitrogen yielded adsorption performance. The process Fe(OH)3@CNFs followed...
Polycystic kidney disease (PKD) is the major cause of failure and mortality in humans. It has always been suspected that development cystic shares features with tumorigenesis, although evidence unclear.We crossed p53 mutant mice (p53N236S, p53S) Werner syndrome analyzed pathological phenotypes. The RNA-seq, ssGSEA analysis, real-time PCR were performed to dissect gene signatures involved phenotypes.We found enlarged kidneys fluid-filled cysts offspring a genotype G3mTerc-/-WRN-/-p53S/S...
The effect of different DNA extraction protocols on activated sludge yield and bacterial community composition was evaluated by temperature gradient gel electrophoresis (TGGE). Nine procedures to extract were compared—sonication (30s), sonication (40s), (50s), freezing‐thawing, bead milling, sodium dodecyl sulfate (SDS)‐lysozyme, SDS‐proteinase K, SDS‐lysozyme‐proteinase, a commercial kit. It found that the TGGE profiles band numbers made significant differences via various methods. purity...
The G-rich DNA, such as telomere, tends to form G-quadruplex (G4) structure, which slows down the replication fork progression, induces stress, and becomes chromosome fragile sites. Here we described a molecular strategy that cells developed overcome DNA stress via helicase regulation. p53N236S (p53S) mutation has been found in Werner syndrome mouse embryo fibroblast (MEFs) escaped from senescence, could be driving force for cell escaping senescence. We revealed p53S transcriptionally...
Anti-aging and tumorigenesis share common genes pathways, thus targeting these as part of anti-aging interventions carries the risk tumorigenesis. It is essential to understand gene signatures that balance aging. To achieve this goal, we analyzed RNA-sequencing data from three non-tumorigenic immortalized cell lines spontaneously escaped senescence. By single sample set enrichment assay (ssGSEA) GSEA analysis, found both growth signaling (E2F targets, MYC targets) tumor surveillance...
Summary Our previous study reported a missense mutation (Tyr530Ser) and splicing site (IVS16–1G>T) in blood coagulation factor V (FV) gene two-year-old Chinese boy. However, the linkage between mutations severe FV deficiency underlying mechanism has not been elucidated. The present was designed to investigate effect of two possible pathogenetic mechanism. procoagulant activity showed tremendous decrease patient with mutations. bioinformatics analyses predicted that IVS16–1G>T may cause...
Missense mutations in the p53 coding gene cause loss and gain of function. We have identified a hotspot mutation, N236S , which results aggressive progression tumorigenesis knock‐in mouse model. To understand biological significance we performed Ch IP ‐on‐chip combined with microarray assay to profile regulated expression pattern. could classify mutant function into six categories. Among these, reveal new aspect function, enhancement wild‐type has not been reported previously. also show...
Studies on the crosstalk between muscle and adipose tissue can provide beneficial help in elucidating pathogenesis treatment of obesity-related diseases [1]. In this data article, we performed RNA sequence analysis mRNA isolated from epididymal gastrocnemius obese rats. Twenty-two samples were selected for gene expression analysis. Raw Illumina Hiseq™ platform sequencer was used differential using DESeq deposited GEO public repository under accession number GSE237950. With economic...