A5006921758- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- CAR-T cell therapy research
- 3D Printing in Biomedical Research
- Genetics and Neurodevelopmental Disorders
- CRISPR and Genetic Engineering
- Cell Image Analysis Techniques
- Colorectal Cancer Surgical Treatments
- Pancreatic and Hepatic Oncology Research
- Colorectal and Anal Carcinomas
- Lung Cancer Treatments and Mutations
- Autism Spectrum Disorder Research
- Heat shock proteins research
- Prostate Cancer Treatment and Research
- Immune Cell Function and Interaction
- Colorectal Cancer Treatments and Studies
- Gastric Cancer Management and Outcomes
- Immunotherapy and Immune Responses
Fudan University Shanghai Cancer Center
2022-2025
Shanghai Medical College of Fudan University
2022-2025
Shanghai Clinical Research Center
2024-2025
Fudan University
2020
Zhongshan Hospital
2020
State Key Laboratory of Medical Neurobiology
2020
Abstract There is no effective method to predict chemotherapy response and postoperative prognosis of colorectal cancer liver metastasis (CRLM) patients. Patient‐derived organoid (PDO) has become an important preclinical model. Herein, a living biobank with 50 CRLM organoids derived from primary tumors paired metastatic lesions successfully constructed. PDOs the multiomics levels (histopathology, genome, transcriptome single‐cell sequencing) are comprehensively analyzed confirmed that this...
Organoids are three-dimensional structures that closely recapitulate tissue architecture and cellular composition, thereby holding great promise for organoid-based drug screening. Although growing in provides the possibility organoids to main features of corresponding tissues, it makes incommodious imaging two-dimensional identifying surviving from surrounding dead cells after being treated by irradiation or chemotherapy. Therefore, significant work remains establish high-quality controls...
Abstract Background Radiotherapy (RT) is an essential treatment for colorectal cancer (CRC), yet the factors influencing radiosensitivity remain unclear. In quest to enhance therapeutic efficacy in CRC, interplay between genetic mutations and RT sensitivity has emerged as a pivotal enigmatic area. Methods We harness fidelity of patient-derived organoids (PDOs) dissect molecular landscape radiosensitivity, with particular emphasis on BRAF V600E mutations. To further investigate, cohort 9...
Abstract Adding irinotecan to neoadjuvant chemoradiotherapy (nCRT) in locally advanced rectal cancer (LARC) increases the pathologic complete response (pCR) rate but brings more toxicities. Robust biomarkers predict irinotecan‐based nCRT are extremely necessary for selecting right patients. Our previous study suggests that patient‐derived tumor organoids (PDTOs) sensitivity matches patient responses. In this study, we investigated whether PDTOs can (CR) and survival. Eligible patients...
Poor penetration of transferred T cells represents a critical factor impeding the development adoptive cell therapy in solid tumors. We demonstrated that iRGD-antiCD3 modification promoted both infiltration and activation our previous work. Interest low-dose radiotherapy has recently been renewed due to its immuno-stimulatory effects including recruitment. This study aims explore synergistic between iRGD-antiCD3-modified cells.
Conditional gene inactivation and restoration are powerful tools for studying functions in the nervous system modeling neuropsychiatric diseases. The combination of two is necessary to interrogate specific cell types within defined developmental stages. However, very few methods animal models have been developed such purpose. Here we present a versatile method conditional <i>in situ</i> through reversibly inverting critical part its endogenous genomic sequence by Cre- Flp-mediated...
e15598 Background: 5-FU-based regimens are the mainstay treatment for colorectal cancer. However, limited response rate to hampered increase in efficacy of locally advanced rectal During drug screening cancer organoid biobank (n = 106), we found out that more than half organoids were resistant 5-FU monotherapy and sensitive irinotecan maintained status combined with (combination-sensitive), while a quite proportion them turn become remarkably plus (combination-resistant). Some studies...