A5078564809- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Viral Infections and Vectors
- Plant and Fungal Interactions Research
- CRISPR and Genetic Engineering
- Pediatric health and respiratory diseases
- Poxvirus research and outbreaks
- Plant Virus Research Studies
- Rabies epidemiology and control
- Bacillus and Francisella bacterial research
- Zoonotic diseases and public health
- Respiratory viral infections research
- Hepatitis C virus research
- Gut microbiota and health
- Viral Infections and Outbreaks Research
- Autophagy in Disease and Therapy
Chinese Academy of Agricultural Sciences
2022-2024
Jilin Academy of Agricultural Sciences
2024
Jilin University
2019-2022
Academy of Military Medical Sciences
2017-2021
Abstract Wildlife is reservoir of emerging viruses. Here we identified 27 families mammalian viruses from 1981 wild animals and 194 zoo collected south China between 2015 2022, isolated characterized the pathogenicity eight Bats harbor high diversity coronaviruses, picornaviruses astroviruses, a potentially novel genus Bornaviridae . In addition to reported SARSr-CoV-2 HKU4-CoV-like viruses, picornavirus respiroviruses also likely circulate bats pangolins. Pikas new clade Embecovirus...
Bats are associated with the circulation of most mammalian filoviruses (FiVs), pathogenic ones frequently causing deadly hemorrhagic fevers in Africa. Divergent FiVs have been uncovered Chinese bats, raising concerns about their threat to public health. Here, we describe a long-term surveillance track bat at orchards, eventually resulting identification and isolation FiV, Dehong virus (DEHV), from Rousettus leschenaultii bats. DEHV has typical filovirus-like morphology wide spectrum cell...
Porcine postweaning multisystemic wasting syndrome (PMWS) caused by porcine circovirus type 2 (PCV2) is a disease causing severe economic losses annually worldwide to the pig industry. PCV2 infection was first reported in China 2000, and currently has three major genotypes, PCV2a, b d, circulating this country. To further elucidate origin prevalence of China, 123 clinical tissue samples collected 25 provinces between 1990 1999 were analysed PCV2-specific PCR, resulting identification 23...
PCV3 is an emerging swine virus associated with porcine dermatitis and nephropathy syndrome (PDNS), reproductive failure, respiratory diseases systematic inflammation. Although first identified in 2015, the earliest case has been traced back to 2009 United States. In China, infection was detected but little information available about its occurrence prevalence there before 2015. this study, 200 clinical samples collected from 20 provinces, five autonomous regions four municipalities between...
Getah virus (GETV) is a mosquito-borne that widely distributed in Asian countries including China, which the first case of equine GETV infection was reported Guangdong province August 2018. In this study, GETVs were detected two classical swine fever virus-positive samples collected from herds Foshan city, province, Infection porcine PK-15 cells produced rapid cytopathic effects (CPEs), shrinking, rounding and detaching, peak titre 109.3 TCID50/ml occurred at 24 hr post-infection. Electron...
Classical swine fever (CSF) is a highly contagious infectious disease and causes significant economic losses for the pig industry worldwide. The objective of this study was to determine whether small molecule metabolites contribute pathogenesis CSF. Birefly, serum metabolomics CSFV Shimen strain-infected piglets were analysed by ultraperformance liquid chromatography/electrospray ionization time-of-flight mass spectrometry (UPLC/ESI-Q-TOF/MS) in combination with multivariate statistical...
Abstract Background Virus neutralization test (VNT) is widely used for serological survey of classical swine fever (CSF) and efficacy evaluation CSF vaccines. However, VNT a time consuming procedure that requires cell culture live virus manipulation. C-strain vaccine the most frequently control prevention. In this study, we presented neutralizing monoclonal antibody (mAb) based competitive enzyme-linked immunosorbent assay (cELISA) with emphasis on replacement post–vaccination monitoring....
Many new astroviruses have been identified in humans and other animals recent years, but only a few successfully isolated for extensive biological study. Here, we report an unusual isolation of porcine astrovirus 5 (PAstV5) strain from clinical classical swine fever virus (CSFV)-infected tissue sample. Incubation PK-15 cells with extract the CSFV-positive resulted unexpected cytopathic effects (CPEs), high-throughput viromic sequencing PAstV5 circovirus type 2 (PCV2) as well CSFV culture....
Classical swine fever virus (CSFV) can utilize diverse host signaling pathways for its replication; however, the cross talk between mammalian target of rapamycin (mTOR) and CSFV remains unknown. Here, we describe potential role mTOR complex 1 (mTORC1) in promoting replication via virus-induced hypophosphorylation Akt/mTORC1/S6 pathway, especially at an early stage viral infection. Conversely, activation mTORC1 inhibited CSFV. Furthermore, revealed underlying mechanisms pathway mediating...
Classical swine fever virus (CSFV) is a major animal pathogen threatening the global pork industry. To date, numerous anti-CSFV monoclonal antibodies (mAbs) and their recognizing epitopes have been reported. However, few mAbs were systematically characterized for capacity to differentiate field CSFV isolates from CSF vaccine strains, molecular basis associated with antigenic differences between vaccines still largely unknown. In present study, recombinant structural glycoproteins E2 of both...
Currently, the Birnaviridae family contains four genera with all members identified from birds, fishes, and insects only. The present study reports a novel birnavirus unexpectedly classical swine fever virus-infected pigs by viral metagenomic analysis, which is, therefore, named as porcine (PBRV). Follow-up reverse transcription-polymerase chain reaction (RT-PCR) screening of archived tissues diseased 16 PBRV strains nine provinces/autonomous regions in China spanning 21 years (1998-2019),...
Ephemeroviruses are arthropod-borne rhabdoviruses within Ephemerovirus genus and have been isolated exclusively from cattle haematophagous arthropods (mosquitoes biting midges) without any member detected or up to date pigs, although some serological surveys indicated that pigs may be a silent host for ephemerovirus infection. Here, many viral reads annotated to, but genetically distinct from, the existing members identified in meta-transcriptomic data of two clinical classical swine fever...
Members of the genus Hepacivirus have a broad range hosts, with at least 14 species identified. To date, highly pathogenic hepacivirus causing severe disease in animals has not been found. Here, by using high-throughput sequencing, new was identified as dominant and virus acute hepatitis outbreaks bamboo rats (Rhizomys pruinosus), ≈80% mortality; this emerged February 2020 two rat farms China. Hepaciviral genome copies liver were significantly higher than other organs. Genomic sequences...
<title>Abstract</title> Bovine viral diarrhea virus (BVDV) is commonly detected in biological products such as vaccines and serum. In this study, we have BVDV commercial foetal bovine order to determine whether the serum contained infectious or genes, inoculated sample into MDBK cells. After six passages, results of indirect immunofluorescence assay confirmed that was contaminated with an strain BVDV, designated BI-2023. The complete genome sequence isolate 12,273 nt. Subsequent analysis...
ABSTRACT Rabies is an acute lethal disease causing by the neurotropic virus rabies (RABV). immune globulin (RIG) as indispensable component of postexposure prophylaxis (PEP) always faces with great challenges in terms costs, stability and safety. Our objective to develop a novel potential fully human monoclonal antibodies (mAbs) cocktail for improvement PEP. The neutralizing mAbs were screened using humanized antibody mice (CAMouse HG ). Then, two 26–12 G 5–7 selected activity RABV...
Classical swine fever can be controlled effectively by vaccination with C-strain vaccine. In this study, we developed a novel competitive enzyme-linked immunosorbent assay (cELISA) based on Erns specific monoclonal antibody (mAb 1504), aiming to serologically measure immune responses vaccine in pigs, and finally make the become DIVA-compatible The cELISA system was established strategy that mAb 1504 will compete induced antibodies pig serum bind protein. optimized further evaluated testing...
The complete genome sequence of a sub-subgenotype 2.1i isolate classical swine fever virus (CSFV), GD317/2011, was determined. Notably, GD317/2011 is distant from the 2.1b HEBZ at genes Erns, E1, E2, P7, NS2, NS5A and 3'-nontranslated region (3'-NTR) but closely related to that Npro, Core, NS3, NS4A, NS4B, NS5B.
Abstract Background: Virus neutralization test (VNT) is widely used for serological survey of classical swine fever (CSF) and efficacy evaluation CSF vaccines. However, VNT a time consuming procedure that requires cell culture live virus manipulation. C-strain vaccine the most frequently control prevention. In this study, we presented neutralizing monoclonal antibody (mAb) based competitive enzyme-linked immunosorbent assay (cELISA) with emphasis on replacement post–vaccination monitoring....