A5054139831- Advanced MRI Techniques and Applications
- Epilepsy research and treatment
- Neuroscience and Neuropharmacology Research
- Medical Imaging Techniques and Applications
- Functional Brain Connectivity Studies
- Neurotransmitter Receptor Influence on Behavior
- EEG and Brain-Computer Interfaces
- Schizophrenia research and treatment
- Receptor Mechanisms and Signaling
- Radiopharmaceutical Chemistry and Applications
- Diet and metabolism studies
- Nicotinic Acetylcholine Receptors Study
- Treatment of Major Depression
- Autoimmune Neurological Disorders and Treatments
- Pharmacological Effects and Toxicity Studies
- Parkinson's Disease Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological disorders and treatments
- Neonatal and fetal brain pathology
- Peripheral Neuropathies and Disorders
- Bipolar Disorder and Treatment
- Ion channel regulation and function
- Glioma Diagnosis and Treatment
- Obsessive-Compulsive Spectrum Disorders
- Drug Transport and Resistance Mechanisms
Rigshospitalet
2016-2025
University of Copenhagen
2015-2025
Copenhagen University Hospital
2016-2025
Institute of Neurobiology
2006-2023
Epilepsy Foundation
2010-2023
Smerteklinikken
2022
Utrecht University
2020
University Medical Center Utrecht
2020
Aarhus University Hospital
2019
Hvidovre Hospital
2019
Abstract Epilepsy is one of the most common neurological disorders, yet its pathophysiology poorly understood due to high complexity affected neuronal circuits. To identify dysfunctional subtypes underlying seizure activity in human brain, we have performed single-nucleus transcriptomics analysis >110,000 transcriptomes derived from temporal cortex samples multiple lobe epilepsy and non-epileptic subjects. We found that largest transcriptomic changes occur distinct several families...
Parkinsonism and attention deficit hyperactivity disorder (ADHD) are widespread brain disorders that involve disturbances of dopaminergic signaling. The sodium-coupled dopamine transporter (DAT) controls homeostasis, but its contribution to disease remains poorly understood. Here, we analyzed a cohort patients with atypical movement identified 2 DAT coding variants, DAT-Ile312Phe presumed de novo mutant DAT-Asp421Asn, in an adult male early-onset parkinsonism ADHD. According single-photon...
Recently, a hexanucleotide ( GGGGCC ) repeat expansion in the first intron of C9ORF72 was reported as cause chromosome 9p21‐linked frontotemporal dementia‐amyotrophic lateral sclerosis FTD‐ALS ). We here report prevalence hospital‐based cohort and associated clinical features indicating wider spectrum disease than previously described. studied 280 patients screened for mutations genes involved early onset autosomal dominant inherited dementia disorders. A repeat‐primed polymerase chain...
Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in human brain and plays a key role several functions neuropsychiatric disorders such as anxiety, epilepsy, depression. For decades, vivo ex techniques have been used to highlight mechanisms of GABA system, however, no studies currently combined create high-resolution multimodal view system. Here, we present quantitative atlas benzodiazepine receptor sites (BZR) located on postsynaptic ionotropic GABAA receptors...
Drug-resistant focal epilepsy is often caused by cortical dysplasias (FCDs). The distribution of these lesions across the cerebral cortex and impact lesion location on clinical presentation surgical outcome are largely unknown. We created a neuroimaging cohort patients with individually mapped FCDs to determine factors associated predictors postsurgical outcome.
Abstract One outstanding challenge for machine learning in diagnostic biomedical imaging is algorithm interpretability. A key application the identification of subtle epileptogenic focal cortical dysplasias (FCDs) from structural MRI. FCDs are difficult to visualize on MRI but often amenable surgical resection. We aimed develop an open-source, interpretable, surface-based machine-learning automatically identify heterogeneous data epilepsy surgery centres worldwide. The Multi-centre Epilepsy...
The effect of hyperinsulinemia on glucose blood-brain barrier (BBB) transport and cerebral metabolism (CMRglc) was studied using the intravenous double-indicator method positron emission tomography [18F]fluorodeoxyglucose as tracer (PET-FDG). Sixteen normal healthy control subjects (25 +/- 4 years old) were twice during a euglycemic euglycemic-hyperinsulinemic condition. Our hypothesis that high physiologic levels insulin did not affect BBB or net glucose. During infusion, arterial plasma...
During starvation, brain energy metabolism in humans changes toward oxidation of ketone bodies. To investigate if this shift is directly coupled to circulating blood concentrations bodies, we measured global cerebral flow (CBF) and carbohydrate with the Kety-Schmidt technique before during intravenous infusion acute hyperketonemia (mean beta-hydroxybutyrate concentration 2.16 mM), uptake ketones increased from 1.11 5.60 mumol.100 g-1.min-1, counterbalanced by an equivalent reduction glucose...
During prolonged starvation, brain energy requirements are covered in part by the metabolism of ketone bodies. It is unknown whether short-term starvation a few days' duration may lead to reduced glucose due change toward body consumption. In present study we measured cerebral and bodies nine healthy volunteers before after 3.5 days starvation. Regional was dynamic positron emission tomography using [ 18 F]2-fluoro-2-deoxy-D-glucose. The mean value K* 1 gray white matter increased 12% (p...
The pharmacological efficacy of serotonergic-acting drugs suggest that patients with obsessive–compulsive disorder (OCD) may have alterations in their cerebral serotonergic (5-HT) receptor system, and previous neuroimaging studies OCD shown abnormalities several fronto-subcortical regions. In this study we investigated 5-HT2A binding 15 untreated age- gender-matched healthy volunteers by magnetic resonance imaging [18F]altanserin positron emission tomography (PET). Eleven the were rescanned...
The blood-brain barrier (BBB) permeability for glucose and beta-hydroxybutyrate (beta-OHB) was studied by the intravenous double-indicator method in nine healthy subjects before after 3.5 days of starvation. In fasting, mean arterial plasma decreased concentration beta-OHB increased, whereas cerebral blood flow remained unchanged. permeability-surface area product BBB transport from to brain (PS1) increased 55 +/- 31%, no significant change back (PS2) found. PS1 constant during expected...
Postmortem investigations and the receptor affinity profile of atypical antipsychotics have implicated participation serotonin(2A) receptors in pathophysiology schizophrenia. Most postmortem studies point toward lower cortical binding schizophrenic patients. However, vivo report conflicting results, presumably because sample sizes been small or patients who were not antipsychotic-naive included. Furthermore, relationships between binding, psychopathology, central neurocognitive deficits...
<h3>Objective</h3> To determine the diagnostic accuracy and clinical utility of electromagnetic source imaging (EMSI) in presurgical evaluation patients with epilepsy. <h3>Methods</h3> We prospectively recorded magnetoencephalography (MEG) simultaneously EEG performed EMSI, comprising electric imaging, magnetic analysis combined MEG-EEG datasets, using 2 different software packages. As reference standard for irritative zone (IZ) seizure onset (SOZ), we used intracranial recordings...
Abstract Optogenetics is one of the most powerful tools in neuroscience, allowing for selective control specific neuronal populations brain experimental animals, including mammals. We report, first time, application optogenetic to human tissue providing a proof-of-concept use optogenetics neuromodulation cortical and hippocampal neurons as possible tool explore network mechanisms develop future therapeutic strategies.
The aim of this study was to describe clinical and paraclinical characteristics all Danish patients who tested positive for anti-voltage-gated potassium channels (VGKC)-complex, anti-leucine-rich glioma-inactivated 1 (LGI1) anti-contactin-associated protein-2 antibodies in the serum/cerebrospinal fluid between 2009 2013 with follow-up interviews 2015 2016.We evaluated antibody status, symptoms leading testing, course disease, suspected diagnosis time admission as well treatment. All magnetic...
Background and purpose Accurate localization of the epileptic focus is essential for surgical treatment patients with drug‐resistant epilepsy. Electric source imaging ( ESI ) increasingly used in pre‐surgical evaluation. However, most previous studies have analysed interictal (II) discharges. Prospective comparing feasibility accuracy II ictal IC are lacking. Methods We prospectively long‐term video‐electroencephalography recordings LTM admitted performed signals using two methods, i.e....
The branched-chain amino acids (BCAAs) leucine, isoleucine, and valine are important nitrogen donors for synthesis of glutamate, the main excitatory neurotransmitter in brain. glutamate carbon skeleton originates from tricarboxylic acid (TCA) cycle intermediate α-ketoglutarate, while group is derived such as BCAAs. Disturbances homeostasis, mainly strongly implicated pathophysiology Alzheimer's disease (AD). divergent BCAA metabolism different cell types human brain poorly understood, so...
Current SPECT radioligands available for in vivo imaging of the dopamine transporter (DAT) also show affinity monoamine transporters other than DAT, especially serotonin (SERT). The effect this lack selectivity is unknown. In study, we compared <sup>123</sup>I-2-β-carbomethoxy-3β-(4-iodophenyl)-<i>N</i>-(3-fluoropropyl)nortropane (<sup>123</sup>I-FP-CIT) and <sup>123</sup>I-<i>N</i>-(3-iodoprop-2E-enyl)-2-β-carbomethoxy-3β-(4-methylphenyl) (<sup>123</sup>I-PE2I). <sup>123</sup>I-FP-CIT has a...