A5100692579- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Mesenchymal stem cell research
- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Acute Myeloid Leukemia Research
- Cancer, Hypoxia, and Metabolism
- Blood groups and transfusion
- Congenital heart defects research
- Cancer-related gene regulation
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Meat and Animal Product Quality
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Adipokines, Inflammation, and Metabolic Diseases
- Circular RNAs in diseases
- Genetic and phenotypic traits in livestock
- Cancer Cells and Metastasis
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
Nanjing Medical University
2018-2025
Jiangsu Province Hospital
2018-2025
Institute of Hematology & Blood Diseases Hospital
2018-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2018-2025
Zhejiang Provincial People's Hospital
2023-2024
WuXi AppTec (China)
2023-2024
Children's Hospital of Fudan University
2019-2024
Anhui Medical University
2017-2024
Beijing University of Chinese Medicine
2021-2024
Hangzhou Medical College
2021-2024
In this study, the effects of lutein on neuroinflammation in lipopolysaccharide (LPS)-activated BV-2 microglia were investigated.The production proinflammatory cytokines tumor necrosis factor-α, interleukin-1β, and nitric oxide was measured culture medium using enzyme immunoassay Griess reagent, respectively. The expression proteins determined Western blot. Pretreatment with (50 μM) prior to LPS (1 μg/mL, 12 h) stimulation resulted a significant inhibition inducible synthase cyclooxygenase-2...
High throughput single-cell RNA-seq has been successfully implemented to dissect the cellular and molecular features underlying hematopoiesis. However, an elaborate comprehensive transcriptome reference of whole blood system is lacking. Here, we profiled transcriptomes 7551 human cells representing 32 immunophenotypic cell types, including hematopoietic stem cells, progenitors mature derived from 21 healthy donors. With high sequencing depth coverage, constructed a transcriptional atlas...
Type 2 resistant starch (RS2) is a fermentable dietary fiber conferring health benefits. We investigated the effects of RS2 on host, gut microbiota, and metabolites in aged mice high-fat diet. In eighteen-month old randomly assigned to control, (HF), or high-fat+20% (HFRS) diet for 16 weeks, reversed weight gain hepatic steatosis induced by Serum fecal LPS, colonic IL-2 IL-4 mRNA expressions decreased while mucin protein increased HFRS compared HF control group. 16s rRNA sequencing microbial...
Severe coronavirus disease 2019 (COVID-19) is often indicated by lymphopenia and increased myelopoiesis; however, the underlying mechanism still unclear, especially alteration of hematopoiesis. It important to explore what extent how hematopoietic stem cells contribute impairment peripheral lymphoid myeloid compartments in COVID-19 patients. In this study, we used single-cell RNA sequencing assess bone marrow mononuclear from patients with blood as control. The results showed that these were...
Aim: Inflammation and fibrosis have been shown to be critical factors in heart failure (HF) progression. Calycosin (Cal) is the major active component of Astragalus mongholicus Bunge has reported therapeutic effects on cardiac dysfunction after myocardial infarction. However, whether Cal could ameliorate infarction (MI)-induced inflammation precise mechanisms remain uncertain. The aim this study explore role HF clarify underlying mechanisms. Methods: For vivo experiments, rats underwent left...
The lipopeptide iturin from Bacillus subtilis has been found to have a potential inhibitory effect on breast cancer, alveolar adenocarcinoma, renal carcinoma, and colon adenocarcinoma. In this study, the of B. lipopeptides (a mixture homologues, concentration 42.75%) inhibit chronic myelogenous leukemia was evaluated using K562 cells. results showed that could completely growth at 100 μM, with an IC50 value 65.76 μM. inhibited profile via three pathways: (1) induction paraptosis indicated by...
Abstract Objectives microRNA‐29 (miR‐29) family have shown different expression patterns in cardiovascular diseases. Our study aims to explore the effect and mechanism of miR‐29 on cardiac development. Materials methods A total 13 patients with congenital heart disease (CHD) 7 controls were included our study. Tissues obtained from right ventricular outflow tract (RVOT) after surgical resection or autopsy. The next‐generation sequencing was applied screen microRNA profiles CHD. Quantitative...
A kind of drug-loaded nanomotors with deep penetration was developed to improve the therapeutic effect ferroptosis on tumor. The were constructed by co-loading hemin and ferrocene (Fc) surface bowl-shaped polydopamine (PDA) nanoparticles. near-infrared response PDA makes nanomotor have high tumor capability. In vitro experiments show that can exhibit good biocompatibility, light heat conversion efficiency, permeability. It is worth noting under catalysis H2O2 overexpressed in...
Metastatic colorectal cancer (mCRC) is a major cause of cancer-related mortality due to the absence effective therapeutics. Thus, it urgent discover new drugs for mCRC. Fucosyltransferase 8 (FUT8) potential therapeutic target with high level in most malignant cancers including CRC. FUT8 mediates core fucosylation CD276 (B7-H3), key immune checkpoint molecule (ICM), FUT8-silence-induced defucosylation at N104 on B7-H3 attracts heat shock protein family A member (HSPA8, also known as HSC70)...
Although some cohort studies have indicated a close association between diabetes and HCC, the underlying mechanism about contribution of to HCC progression remains largely unknown. In study, we applied novel model in SD rat with series high glucose-stimulated cell experiments explore effect glucose environment on metastasis its relevant mechanism. Our results uncovered regulatory by which nuclear translocation metabolic enzyme PKM2 mediated glucose-promoted metastasis. Specifically,...
Abstract This study revealed that iturin A‐like lipopeptides produced by Bacillus subtillis induced both paraptosis and apoptosis in heterogeneous human epithelial colorectal adenocarcinoma (Caco‐2) cells. Autophagy was simultaneously Caco‐2 cells treated with at the early stage inhibited later stage. A western blot analysis showed via a mitochondrial‐dependent pathway, as indicated upregulated expression of apoptotic genes bax bad downregulated antiapoptotic gene bcl‐2. The induction...
Osteosarcoma (OS) is an aggressive bone tumor, and proto-oncogene c-Fos involved in this lethal disease. However, the role molecular mechanism of development progression OS remain enigmatic. As one Wnt family members, Wnt2 closely associated with several malignant tumors. In present study, expression c-Fos, Wnt2, its receptor Fzd9 human tissues, MG63 cell line, osteoblast hFOB 1.19 line was detected by Western blot analysis, immunohistochemical staining, or reverse transcription-polymerase...
Chemoresistance is a leading obstacle in effective management of advanced prostate cancer (PCa). A better understanding the molecular mechanisms involved PCa chemoresistance could improve treatment patients with PCa. In present study, using immune histochemical, chemistry, and precipitation assays cells from individuals benign or malignant established cell lines, we found that oncogenic transcription factor pre-B leukemia homeobox-1 (PBX1) promotes proliferation confers to resistance against...
Metformin, a traditional first-line anti-hyperglycemic agent for diabetes, recently exhibits better antitumor effect in hepatocellular carcinoma (HCC). However, its resistance and tolerance mechanism HCC remains largely unknown. Here, we investigated whether increased matrix stiffness attenuated the intervention effects of metformin on invasion metastasis, explored underlying molecular mechanism.FN-coated gel substrates with 6, 10, 16 kPa, which simulated normal, fibrotic, cirrhotic liver...
Abstract Eltrombopag (EPAG) is an oral thrombopoietin receptor agonist analog with the potential risk to induce liver injury. This prospective registry study evaluated prevalence and severity of hepatic injury in Chinese patients severe aplastic anemia undergoing low-dose EPAG treatment (75 mg/day) context standard immunosuppressive therapy (IST). The incidence acute drug-induced was slightly higher IST + group than at 1st 2nd month, but no statistically significant difference observed: 10%...
Umbilical cord blood (UCB) showcases substantial roles in hematopoietic stem cells (HSCs) transplantation and regenerative medicine. UCB is usually cryopreserved for years before use. Whether how cryopreservation affects its function remain unclear. We constructed single-cell transcriptomic profile of CD34+ progenitor (HSPCs) mononuclear (MNCs) from fresh stored 1-, 5-, 10-, 19- years. Compared to UCB, HSCs multipotent progenitors (MPPs) exhibited more active cell cycle lower HSC/MPP...
An effective preoperative diagnosis between minute pulmonary meningothelial-like nodules (MPMNs) and adenocarcinoma in situ (AIS) can provide clinicians with appropriate treatment strategies. This study aimed to differentiate MPMNs from AIS via computed tomography (CT) radiomics approaches. Clinical imaging data fifty-one patients diagnosed 55 were collected Jiangsu Province Hospital Nanjing First January 2016 December 2022. All underwent chest CT scans before surgery. images segmented...
Abstract Yellow plumage is common in chickens, especially breeds such as the Huiyang Bearded chicken, which indigenous to China. We evaluated colour distribution F1, F2, and F3 populations of an chicken × White Leghorn cross, heredity yellow trait was distinguished from that gold other known colours. Microscopic analysis feather follicles indicated pheomelanin particles were formed but not white feathers. To screen genes related formation particles, we generated transcriptome data 7-...