A5006550760- Cellular Mechanics and Interactions
- Skin and Cellular Biology Research
- Systemic Lupus Erythematosus Research
- Wnt/β-catenin signaling in development and cancer
- Cell Adhesion Molecules Research
- Cell Image Analysis Techniques
- 3D Printing in Biomedical Research
- Heterotopic Ossification and Related Conditions
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Single-cell and spatial transcriptomics
- Systemic Sclerosis and Related Diseases
- Hippo pathway signaling and YAP/TAZ
- Cancer-related gene regulation
- Urticaria and Related Conditions
- Polysaccharides and Plant Cell Walls
- Viral-associated cancers and disorders
- Rheumatoid Arthritis Research and Therapies
- Advanced Fluorescence Microscopy Techniques
- Monoclonal and Polyclonal Antibodies Research
- Body Contouring and Surgery
- Cytokine Signaling Pathways and Interactions
- Dermatological and COVID-19 studies
- Proteoglycans and glycosaminoglycans research
- Nonmelanoma Skin Cancer Studies
- Autoimmune Bullous Skin Diseases
The University of Texas Southwestern Medical Center
2017-2024
Southwestern Medical Center
2019-2024
Center for Systems Biology
2024
Emory University
2012-2016
Winship Cancer Institute
2012
p120-catenin (p120) binds to the cytoplasmic tails of classical cadherins and inhibits cadherin endocytosis. Although p120 regulation internalization is thought be important for adhesive junction dynamics, mechanism by which modulates endocytosis unknown. In this paper, we identify a dual-function motif in consisting three highly conserved acidic residues that alternately serve as p120-binding interface an endocytic signal. Mutation resulted variant was both uncoupled resistant endothelial...
Extracellular matrix (ECM) interactions regulate both the cell transcriptome and proteome, thereby determining fate. Traumatic heterotopic ossification (HO) is a disorder characterized by aberrant mesenchymal lineage (MLin) differentiation, forming bone within soft tissues of musculoskeletal system following traumatic injury. Recent work has shown that HO influenced ECM-MLin receptor signaling, but how ECM binding affects cellular outcomes remains unclear. Using time course transcriptomic...
Vascular endothelial (VE)-cadherin, the major adherens junction adhesion molecule in cells, interacts with p120-catenin and β-catenin through its cytoplasmic tail. However, specific functional contributions of catenins to establishment strong are not fully understood. Here we use bioengineering approaches identify roles cadherin-catenin interactions promoting cellular ability cells spread on an adhesive surface. Our results demonstrate that domain VE-cadherin binds is required for...
Most imaging studies in the biological sciences rely on analyses that are relatively simple. However, manual repetition of analysis tasks across multiple regions many images can complicate even simplest analysis, making record keeping difficult, increasing potential for error, and limiting reproducibility. While fully automated solutions necessary very large data sets, they sometimes impractical small- medium-sized sets common biology. Here we present Slide Set plugin ImageJ, which provides...
Vascular endothelial (VE)-cadherin undergoes constitutive internalization driven by a unique endocytic motif that also serves as p120-catenin (p120) binding site. p120 masks the motif, stabilizing cadherin at cell junctions. This mechanism allows VE-cadherin endocytosis and recycling to contribute adherens junction dynamics without resulting in disassembly. Here we identify an additional drives pathological disassembly associated with endothelial-derived tumor Kaposi sarcoma. Human...
Fast volumetric imaging of large fluorescent samples with high-resolution is required for many biological applications. Oblique plane microscopy (OPM) provides high spatiotemporal resolution, but the field view typically limited by its optical train and pixel number camera. Mechanically scanning sample or decreasing overall magnification system can partially address this challenge, albeit reducing speed spatial respectively. Here, we introduce a novel dual-axis scan unit OPM that facilitates...
Cell segmentation is the fundamental task. Only by segmenting, can we define quantitative spatial unit for collecting measurements to draw biological conclusions. Deep learning has revolutionized 2D cell segmentation, enabling generalized solutions across types and imaging modalities. This been driven ease of scaling up image acquisition, annotation computation. However 3D which requires dense slices still poses significant challenges. Labelling every in slice prohibitive. Moreover it...
Abstract Epidermoid cysts with histopathologic features of human papillomavirus (HPV) infection have been previously reported and are commonly termed verrucous cysts. We report a series eight histopathologically distinct pilar cysts, distinguished from traditional epidermoid by trichilemmal keratinization, as well two hybrid pilar‐epidermoid These lesions contain characteristic stratified epithelial linings abrupt transitions to compact eosinophilic keratin, areas papillomatosis, coarse...
Keratin intermediate filaments form strong mechanical scaffolds that confer structural stability to epithelial tissues, but the reason this function requires a protein family with 54 isoforms is not understood. During skin wound healing, shift in keratin isoform expression alters composition of filaments. How change modulates cellular support epidermal remodeling remains unclear. We report an unexpected effect variation on kinase signal transduction. Increased wound-associated 6A,...
Objective The severity and disease course of cutaneous lupus erythematosus (CLE) are highly variable. Consequently, outcome measures for CLE clinical improvement heterogeneous, complicating treatment decisions therapeutic development. This study characterises identifies the influence thresholds on strengths associations with patient demographic factors. Methods In this pilot cohort study, multivariable models identified factors associated activity skin damage improvement, defined as relative...
PURPOSE: Heterotopic ossification (HO) is the formation of ectopic bone in soft tissues musculoskeletal system following burn and blast wounds. Recent studies highlighted necessity for mechanotransductive (FAK/YAP/TAZ) signaling driving mesenchymal lineage (MLin) cells to form HO, however, it's unclear which cell surface receptors are required extracellular matrix-cell interactions. Here, we identify collagen-binding discoidin domain receptor 2 (DDR2) as a driver HO through FAK/YAP/TAZ...
Cutaneous polyarteritis nodosa (cPAN) is a vasculitis of medium-sized vessels with typically unknown etiology and varied clinical presentation making the diagnosis elusive. Here, we describe unique cPAN mimicking appearance behavior pyoderma gangrenosum.