A5045969029- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Reproductive Biology and Fertility
- Advanced Proteomics Techniques and Applications
- Ion-surface interactions and analysis
- Assisted Reproductive Technology and Twin Pregnancy
- Ovarian function and disorders
- Analytical chemistry methods development
- Sperm and Testicular Function
- Prenatal Screening and Diagnostics
- Advanced Chemical Physics Studies
- Spectroscopy and Quantum Chemical Studies
- Reproductive Health and Technologies
- Mesenchymal stem cell research
- Metabolomics and Mass Spectrometry Studies
- Advanced biosensing and bioanalysis techniques
- Tissue Engineering and Regenerative Medicine
- Protein Structure and Dynamics
- Nanoparticle-Based Drug Delivery
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- Pesticide Residue Analysis and Safety
- Peptidase Inhibition and Analysis
- Epigenetics and DNA Methylation
Korea Research Institute of Bioscience and Biotechnology
2016-2025
Stanford Health Care
2017-2025
Stanford Medicine
2019-2025
UConn Health
2024
Orchid Pharma (India)
2022
Korea Advanced Institute of Science and Technology
2019-2021
Jeju National University
2021
Korea University of Science and Technology
2016-2020
Korea Research Institute of Standards and Science
2020
Stanford University
2019
Abstract Ischaemic heart disease (IHD) is the leading cause of death worldwide. Although myocardial cell plays a significant role in infarction (MI), its underlying mechanism remains to be elucidated. To understand progression MI and identify potential therapeutic targets, we performed tandem mass tag (TMT)-based quantitative proteomic analysis using an mouse model. Gene ontology (GO) gene set enrichment (GSEA) revealed that glutathione metabolic pathway reactive oxygen species (ROS) were...
Abstract Phytochrome B (phyB) is a plant photoreceptor that forms membraneless organelle called photobody. However, its constituents are not fully known. Here, we isolated phyB photobodies from Arabidopsis leaves using fluorescence-activated particle sorting and analyzed their components. We found photobody comprises ~1,500 dimers along with other proteins could be classified into two groups: The first includes directly interact localize to the when expressed in protoplasts, while second...
Commensal bacteria are critically involved in the establishment of tolerance against inflammatory challenges, molecular mechanisms which just being uncovered. All kingdoms life produce aminoacyl-tRNA synthetases (ARSs). Thus far, non-translational roles ARSs have largely been reported eukaryotes. Here, we report that threonyl-tRNA synthetase (AmTARS) gut-associated bacterium Akkermansia muciniphila is secreted and functions to monitor modulate immune homeostasis. Secreted AmTARS triggers M2...
The formation of inorganic nanoparticles has been understood based on the classical crystallization theory described by a burst nucleation, where surface energy is known to play critical role, and diffusion-controlled growth process. However, this nucleation model may not be universally applicable entire nanoparticle systems because different precursors ligands are used during their synthesis. Their intrinsic chemical reactivity can lead pathway that deviates from model. metal oxide one such...
The clinical impact of video capsule endoscopy (VCE) in patients with obscure gastrointestinal bleeding (OGIB) remains undetermined. aim this study was to evaluate the long-term VCE OGIB using a nationwide registry.Data from 305 who underwent for 13 hospitals Korea between January 2006 and March 2009 were analyzed. Prospectively collected registry data reviewed, follow-up by chart review telephone interviews patients. Multivariate regression analyses hazard ratios (HR) performed determine...
The loss of endothelial cells is associated with the accumulation monocytes/macrophages underneath surface arteries, where are prone to mechanical stimulation, such as shear stress. However, impact stimuli on monocytic remains unclear. To assess whether stress affects cell function, we examined expression inflammatory molecules and proteins, whose levels changed following in human THP-1 cells. Shear increased chemokine CCL2, which enhanced migration tumor necrosis factor (TNF)-α interleukin...
Parkinson's disease (PD) is characterised by severe movement defects and the degeneration of dopaminergic neurones in midbrain. The symptoms PD can be managed with dopamine replacement therapy using L-3, 4-dihydroxyphenylalanine (L-dopa), which gold standard for PD. However, long-term treatment L-dopa lead to motor complications. central renin-angiotensin system (RAS) associated development neurodegenerative diseases brain. role RAS remains unclear. Here, we tested co-treatment...
A cryopreservation protocol for human amnion-derived mesenchymal stem cells (HAMs) is required because these cannot survive long periods in culture. The aim of this study was to determine whether vitrification a useful freezing method storage HAMs. HAMs were cryopreserved using method. morphology and viability thawed evaluated by Trypan Blue staining. expression several embryonic cell (ESC) markers flow cytometry, RT–PCR immunocytochemistry. Von Kossa, Oil Red O Alcian staining used asses...
SUMMARY ANSWERThe likelihood of an early pregnancy loss may be lower and is certainly not higher in IVF cycles when compared with published rates biochemical fertile women ≤42 years old.
Aflatoxins (AFs) are highly toxic compounds that can cause both acute and chronic toxicity in humans. Aflatoxin B1 (AFB1) is considered the most of AFs. Therefore, rapid on-site detection AFB1 critical for food safety management. Here, we report grains by a portable surface plasmon resonance (SPR) sensor. For AFB1, an SPR Au chip was sequentially modified cysteine-protein G, antibody, bovine serum albumin (BSA). Then, sample solution AFB1-BSA conjugate were flowed onto serial order. In...
Overcoming chemo‑ and radio‑resistance is a major challenge in pancreatic cancer treatment. Therefore, there an urgent need to discover novel therapeutic approaches avoid cancer. Catechol phytochemical found some fruits vegetables. A few studies have reported on the potential anticancer effects of pure catechol. The present study aimed explore radio‑sensitizing catechol Panc‑1 human cells. cell proliferation, clonogenic survival, invasion, migration were assessed using MTT, migration,...
Targeting undruggable proteins by inducing proximity between E3 ligase and their substrates has emerged as an innovative strategy for tackling challenging diseases. In this study, we identified a novel GSPT1 degrader, 4a (KMG-1068), through screening of our in-house small molecule library. Treatment with demonstrated significant anti-proliferative activity across multiple cell lines, which was diminished co-treatment MLN4924, suggesting the involvement Cullin-containing complex. Quantitative...
Abstract Aim: In order to find the optimal exposure time of cryoprotectant, we performed a comparison vitrification versus slow freezing according degree normal morphology and apoptosis human ovarian follicles. Materials Methods: Eleven patients aged 20–41 years who underwent operative laparoscopy for benign cysts or cesarean section were enrolled in this study. We carried out prospective parallel survival follicles after (slow vitrification) thawing. The strips vitrified with two‐step...
Degree of ionization (DI) in matrix-assisted laser desorption (MALDI) was measured for five peptides using α-cyano-4-hydroxycinnanmic acid (CHCA) as the matrix. DIs were low 10(-4) and 10(-7) CHCA. Total number ions (i.e., peptide plus matrix) same regardless their concentration, setting gas-phase generated from a pure matrix upper limit to that ions. Positively charged cluster too weak support ion formation via such The total by MALDI, CHCA, unaffected pulse energy, invalidating...
Pancreatic cancer (PC) has a high mortality rate due to its poor prognosis and the possibility of surgical resection in patients with disease. Importantly, adjuvant chemotherapy is necessary improve PC prognosis. Chrysin, natural product anti-inflammatory, antioxidant, anticancer properties, been studied for several years. Our previous study demonstrated that chrysin induced G protein-coupled estrogen receptor (GPER) expression regulated activity breast cancer. Herein, we investigated...
Abstract Multiple bacterial genera take advantage of the multifunctional autoprocessing repeats-in-toxin (MARTX) toxin to invade host cells. Secretion MARTX by Vibrio vulnificus , a deadly opportunistic pathogen that causes primary septicemia, precursor sepsis, is major driver infection; however, molecular mechanism via which contributes septicemia remains unclear. Here, we report crystal and cryo-electron microscopy (EM) structures effector duet comprising domain unknown function in first...
Product ion yields in postsource decay and photodissociation at 193 266 nm were measured for some peptide ions without a basic amino acid residue ([Y6 + H]+, [F5 [YPFVEPI H]+) generated by matrix-assisted laser desorption ionization (MALDI). Data indicated statistical nature the dissociation processes. Assuming that formed MALDI are thermal equilibrium temperature T their rate constants specified critical energy (E0) entropy (ΔS‡), method based on kinetic analysis was devised to determine...
Time-of-flight (TOF) mass spectra for a peptide (Y6) were obtained by utilizing matrix-assisted infrared laser desorption ionization (IR-MALDI) with glycerol as the matrix and ultraviolet MALDI α-cyano-4-hydroxycinnamic acid (CHCA), sinapinic (SA), 2,5-dihydroxybenzoic (DHB). Collisional activation during ion extraction exothermicity in gas-phase proton transfer found to be unimportant driving forces in-source (ISD) post-source (PSD) decays, indicating that thermal energy acquired...