A5083492856- Superconducting and THz Device Technology
- Terahertz technology and applications
- Semiconductor Quantum Structures and Devices
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Fungal Biology and Applications
- DNA Repair Mechanisms
- PI3K/AKT/mTOR signaling in cancer
- Photosynthetic Processes and Mechanisms
- Ferroptosis and cancer prognosis
- Mitochondrial Function and Pathology
- Genomics, phytochemicals, and oxidative stress
- Autophagy in Disease and Therapy
- Genomics and Chromatin Dynamics
- Pancreatic and Hepatic Oncology Research
- ATP Synthase and ATPases Research
- Microbial Natural Products and Biosynthesis
- Biochemical and Molecular Research
- Epigenetics and DNA Methylation
- Estrogen and related hormone effects
- Genetics and Neurodevelopmental Disorders
- Plant Pathogens and Fungal Diseases
- Natural product bioactivities and synthesis
Shenzhen University
2016-2025
University of Warwick
2024
Queen Mary University of London
2024
University of Michigan
2024
Michigan United
2024
University of North Carolina at Chapel Hill
2024
Jingchu University of Technology
2024
Southern University of Science and Technology
2024
Soochow University
2024
China Electronics Technology Group Corporation
2017-2023
Abstract Tumour metastasis, the spread of cancer cells from original tumour site followed by growth secondary tumours at distant organs, is primary cause cancer-related deaths and remains poorly understood. Here we demonstrate that inhibition CDK4/6 blocks breast metastasis in triple-negative model, without affecting growth. Mechanistically, identify a deubiquitinase, DUB3, as target CDK4/6; CDK4/6-mediated activation DUB3 essential to deubiquitinate stabilize SNAIL1, key factor promoting...
A proper DNA damage response (DDR) is essential to maintain genome integrity and prevent tumorigenesis. double-strand breaks (DSBs) are the most toxic lesion their repair orchestrated by ATM kinase. activated via MRE11-RAD50-NBS1 (MRN) complex along with its autophosphorylation at S1981 acetylation K3106. Activated rapidly phosphorylates a vast number of substrates in local chromatin, providing scaffold for assembly higher-order complexes that can damaged DNA. While reversible ubiquitination...
BRCA1 is an important mediator of the DNA damage response, which promotes homologous recombination (HR) and antagonizes 53BP1-dependent non-homologous end joining in S/G2 phase. But how this achieved remains unclear. Here, we report that E3 ubiquitin ligase UHRF1 (Ubiquitin-like, with PHD RING finger domains 1) directly participates interplay between 53BP1. Mechanistically, recruited to double-strand breaks (DSBs) by S phase, requires BRCT domain phosphorylated Ser674 UHRF1. Subsequently,...
Triple-negative breast cancer (TNBC) is a highly lethal malignancy with limited therapy options. TWIST1, key transcriptional factor of epithelial-mesenchymal transition (EMT), contributes to self-renewal stem-like cells (CSCs), chemo-resistance, metastasis, and TNBC-related death. However, the mechanism by which TWIST1 deregulated in TNBC remains elusive. Here, USP29 identified as bona fide deubiquitinase TWIST1. The deubiquitination catalyzed required for its stabilization subsequent EMT...
Ferroptosis has been reported to play a role in rheumatoid arthritis (RA). Sulfasalazine, common clinical treatment for ankylosing spondylitis, also exerts pathological influence on the progression of including induced ferroptosis fibroblast-like synoviocytes (FLSs), which result perturbated downstream signaling and development RA. The aim this study was investigate underlying mechanism so as provide novel insight
Much recent attention has been focused on Aurora C, the third member of mammalian kinases family that plays significant roles in mitosis. We report here using sensitive RT‐PCR to amplify C‐terminal, we found C is not only expressed highly testis, but also among 16 other human tissues a broad‐spectrum way. as chromosomal passenger protein, co‐localized with B and Survivin mitotic cells. can be associated vivo directly binds vitro . Over‐expression catalytically inactive mutant impaired...
Histone H2B O-GlcNAcylation is an important post-translational modification of chromatin during gene transcription. However, how this epigenetic regulated remains unclear. Here we found that the energy-sensing adenosine-monophosphate-activated protein kinase (AMPK) could suppress histone O-GlcNAcylation. AMPK directly phosphorylates O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT). Although phosphorylation does not regulate enzymatic activity OGT, it inhibits OGT–chromatin...
DBC1 (deleted in breast cancer 1), also known as CCAR2 or KIAA1967, is an important negative regulator of SIRT1 and cellular stress response. Although the Dbc1 gene localizes at a region that homozygously deleted cancer, its role tumorigenesis remains unclear. It has been suggested to be either tumor suppressor oncogene. Therefore, function needs further explored. Here, we report knockout mice are prone, suggesting functions vivo. Our data suggest increased incidence independent Sirt1....
The von Hippel-Lindau tumor suppressor pVHL is an E3 ligase that targets hypoxia-inducible factors (HIFs). Mutation of VHL results in HIF up-regulation and contributes to processes related progression such as invasion, metastasis, angiogenesis. However, very little known with regard post-transcriptional regulation pVHL. Here we show WD repeat SOCS box-containing protein 1 (WSB1) a negative regulator through WSB1's activity. Mechanistically, WSB1 promotes ubiquitination proteasomal...
Abstract Sexual reproduction is the basic way to form high genetic diversity and it beneficial in evolution speciation of fungi. The global teleomorphic species Ascomycota has not been estimated. This paper estimates number for sexual ascomycetes based on five different estimation approaches, viz. by numbers described fungi, fungus:substrate ratio, ecological distribution, meta-DNA barcoding or culture-independent studies previous Ascomycota. assumptions were made with currently most...
a 五邑大学生物科技与大健康学院 广东江门 529020) ( b 深圳大学生命与海洋科学学院 广东深圳 518060) c 兰州大学药学院 兰州 730000) 摘要 合成了系列 2β-acetoxyferruginol 去醋酸基骨架衍生物(1～24), 并测定了其 α-葡萄糖苷酶抑制活性.结果表明: 化合物 1～24 均有较好的 α-葡萄糖苷酶抑制作用.其中(3R,4aS,10aS)-6-羟基-1,1,4-三甲基-1,2,3,4,4a,9,10,10-八氢邻蒽-3-基-4-(三氟甲基)苯甲酸酯(15)抑制 α-葡萄糖苷酶活性最强[IC50＝(23.91±2.34)μmol/L], 是阿卡波糖抑制活性的 23.6 倍. 构效关系分析表明三氟甲基的引入更有利于提高化合物的活性.动力学结果显示化合物 15 为可逆非竞争性的 α-葡 萄糖苷酶抑制剂.3D 荧光结果表明化合物 与 α-葡萄糖苷酶的结合可改变 α-葡萄糖苷酶的构象.分子对接结果显示化
DNA end resection is a highly regulated and critical step in double-stranded break (DSB) repair. In higher eukaryotes, DSB initiated by the collaborative action of CtIP MRE11-RAD50-NBS1 (MRN) complex. Here, we find that deubiquitylating enzyme USP4 directly participates homologous recombination (HR). confers resistance to damage-inducing agents. Mechanistically, interacts with MRN via specific, conserved region catalytic domain USP4, respectively, regulates recruitment sites damage. We also...
Abstract The aberrant upregulation of Yes-associated protein 1 (YAP1) in a variety solid cancers contributes to tumor progression and poor clinical outcomes, rendering it an appealing therapeutic target. However, effective therapies directly target YAP1 remain challenging. In this study, we perform high-throughput screening identify Casein kinase II (CK2) as uncharacterized upstream regulator turnover cancer cells ovarian several other types. Pharmacological inhibition by Silmitasertib or...
Abstract Colorectal cancer (CRC) is among the most prevalent and deadly cancers worldwide. The Yes‐associated protein 1 (YAP1) frequently dysregulated in cancers, contributing to stemness, chemoresistance, cancer‐related death. However, strategies directly targeting YAP1 have not yet been successful because of lack active binding pockets unregulated toxicity. In this study, our Food Drug Administration (FDA)‐approved drug screening reveals that abemaciclib, a cyclin‐dependent kinase 4/6...
The plant Artemisia annua produces the anti-malarial compound artemisinin. Although transcriptional regulation of artemisinin biosynthesis has been extensively studied, its post-translational regulatory mechanisms, especially that protein phosphorylation, remain unknown. Here, we report an ABA-responsive kinase (AaAPK1), a member SnRK2 family, is involved in regulating biosynthesis. physical interaction AaAPK1 with AabZIP1 was confirmed by multiple assays, including yeast two-hybrid,...
(±)-Lucidumone (1), an enantiomeric meroterpenoid possessing unprecedented skeleton comprising a fused 6/5/6/6/5 polycyclic system, was isolated from Ganoderma lucidum and structurally identified. The absolute configuration of (−)-1 assigned by single-crystal X-ray crystallography. A plausible biosynthetic pathway for 1 is proposed. chemical biology approach reveals that selectively inhibits COX-2 directly binding with amino acid residue Tyr385, representing new structure scaffold inhibitors.
Elucidating mechanisms of chemoresistance is critical to improve cancer therapy, especially for the treatment pancreatic ductal adenocarcinoma (PDAC). Genome-wide association studies have suggested less studied gene HEAT repeat-containing protein 1 (HEATR1) as a possible determinant cellular sensitivity different chemotherapeutic drugs. In this study, we assessed hypothesized link in PDAC, where HEATR1 expression downregulated significantly. silencing PDAC cells increased resistance...