A5102719015- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Cellular Mechanics and Interactions
- Ocular Oncology and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal Imaging and Analysis
- Cancer-related gene regulation
- Plant nutrient uptake and metabolism
- Drug Transport and Resistance Mechanisms
- Biological and pharmacological studies of plants
- Antioxidant Activity and Oxidative Stress
- Plant Stress Responses and Tolerance
- Genomics, phytochemicals, and oxidative stress
- Plant Molecular Biology Research
- Silk-based biomaterials and applications
- Autophagy in Disease and Therapy
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Knee injuries and reconstruction techniques
- Endoplasmic Reticulum Stress and Disease
- Traditional Chinese Medicine Analysis
- Retinoids in leukemia and cellular processes
- Renal and related cancers
- Ocular Diseases and Behçet’s Syndrome
Hunan Agricultural University
2025
Sun Yat-sen University
2012-2024
Key Laboratory of Guangdong Province
2023
Abstract Retinoblastoma (RB) is a common intraocular malignancy in children. Due to the poor prognosis of RB, it crucial search for efficient diagnostic and therapeutic strategies. Studies have shown that methyltransferase‐like 3 (METTL3), major RNA N (6)‐adenosine methyltransferase, closely related initiation development cancers. Nevertheless, whether METTL3 associated with RB remains unexplored. Therefore, we investigated function mechanisms regulation progression. We manipulated...
Oxidative damage is a key factor for the pathogenesis of age‑related macular degeneration (AMD), therefore, anti-oxidative stress valuable method prevention or treatment AMD. The aim present study was to reveal protective mechanism lutein on retinal pigment epithelium (RPE) cells subjected oxidative stress. Acute epithelial 19 (ARPE‑19) were exposed induced by H2O2 following pretreatment. activities caspases, level intracellular reactive oxygen species (ROS) and cell cycle analyzed using...
Plants frequently encounter relatively low and fluctuating potassium (K+) concentrations in soil, with roots serving as primary responders to this stress. Histone modifications, such de-/acetylation, can function epigenetic markers of stress-inducible genes. However, the signaling network between histone modifications low-K+ (LK) response pathways remains unclear. This study investigated regulatory role Deacetylase Complex 1 (HDC1) root growth Arabidopsis thaliana under K+ deficiency Using a...
Oxidative stress-induced damage to and dysfunction of retinal pigment epithelium (RPE) cells are important pathogenetic factors age-related macular degeneration (AMD); however, the underlying molecular mechanism is not fully understood. Long noncoding RNAs (lncRNAs) have roles in various biological processes. In this study, using an oxidative model RPE cells, we identified a novel oxidation-related lncRNA named CYLD-AS1. We further revealed that expression CYLD-AS1 was increased RPEs during...
Age-related macular degeneration (AMD) is characterized by the degenerative senescence in retinal pigment epithelium (RPE) and photoreceptors, which accompanied accumulation of iron ions aging retina. However, current models acute oxidative stress are still insufficient to simulate gradual progression AMD. To address this, we established chronic injury exposing aRPE-19 cells, 661W mouse retina ion overload over time. Investigations at levels cell biology molecular were performed. It was...
Proliferative vitreoretinopathy (PVR) is a disease caused by dedifferentiation, translocation and proliferation of several types local cells. These cells form fibrocellular membranes resulting in detachment retinal vision loss. PVR occurs 8%-10% patients undergoing primary (RD) surgery becomes major obstacle for successful RD repair. Retinal pigment epithelial (RPE) are among the which consist membranes. Reproliferation Epithelial-mesenchymal transition (EMT) pathological alteration RPE...
Background: Doxorubicin is a widely used chemotherapy drug for the treatment of variety cancers, however it also has serious side effects such as anaphylaxis and heart damage. Therefore, it's very important to understand downstream molecular pathways that are essential function in cancer treatment. Methods: HeLa S3 cells were treated with different concentrations 24 hours. Then, mRNA levels Notch pathway components determined by Real-Time qRT-PCR. Lentiviral transfection was up-regulate...
To investigate whether dexamethasone has an effect on functional expression of p-glycoprotein in cultured human RPE and, if so, this occurs through interaction with glucocorticoid receptor (GR) and pregnane X (PXR).The D407 was treated increasing concentrations and/or RU486 for various time periods up to 24 hours. Treated cells were collected cell viability, expressions PXR, rhodamine 123 accumulation assays. GR plasmid rifampicin chosen the relationship GR/PXR activation...
The Notch signaling is an evolutionarily conserved cell‐cell communication pathway that plays critical roles in the proliferation, survival, apoptosis, and fate determination of mammalian cells. Retinal pigment epithelial (RPE) cells are responsible for supporting function neural retina maintaining vision. This study investigated RPE We found members components were differentially expressed Furthermore, blockage inhibited migration proliferation reduced expression levels certain target...
Inhibition of p-glycoprotein under hyperglycemic conditions has been reported in various barrier tissues including blood-brain barrier, intestine, and kidney, linked to significant clinical complications. However, whether this is also true for the outer blood-retinal constituted by retinal pigment epithelium, or a role pathogenesis diabetic retinopathy not yet clear. In study, using cultured human epithelium cell line D407, we found that high glucose exposure induced decrease expression both...
Age-related macular degeneration (AMD) is a leading cause of blindness and progressive loss central vision in the elderly population. The important factor AMD pathogenesis retinal pigment epithelial (RPE) cells by oxidative stress. Inactivation PTEN can disrupt intercellular adhesion RPE cells, but mechanism stress less known. Here we presented evidence that UVB-mediated induced apoptosis ARPE-19 cells. Downregulation expression UVB-irradiative triggered DNA damage increased level...
Age-related macular degeneration (AMD), which is the leading cause of blindness among elderly in western societies, majorly accompanied by retinal pigment epithelium (RPE) degeneration. Because irreversible RPE cell loss oxidative stress, it crucial to search for available drugs atrophic (dry) AMD. RNA-Seq analysis revealed that genes related aging and mitochondrial health were differentially expressed under Arbutin treatment, whereas compared injury, our study demonstrated substantially...
The encouraging response and improved survival of acute promyelocytic leukemia patients following retinoic acid treatment has rendered differentiation therapy an attractive option in cancer treatment. Given that terminal represents a considerable barrier retinoblastoma tumorigenesis significantly higher spontaneous degeneration rate compared with other tumors (1,000-fold change), promising alternative the retinoblastoma. However, full potential still unknown. present study was designed to...
Selective covalent CDK7 inhibitor THZ1 is a promising potential anti-tumor drug in many kinds of cancers.Epithelial-mesenchymal Transition (EMT) highly related to cancer initiation, development, invasion and metastasis other pathogenesis processes.We treated cell line Hela229 three retinoblastoma lines so-RB50, WERI-Rb-1, Y79 with gradient concentration THZ1, found that could inhibit viability EMT, suggesting may be for human cervical treatment.Our results verified the role EMT first time,...
Age-related macular degeneration (AMD) is the leading cause of irreversible blindness in elderly. The pathogenesis dry AMD remains indistinct and mechanism retinal pigment epithelium (RPE) cells death controversial. aim present study was to investigate functions Notch signaling ultraviolet B (UVB)-induced damage RPE cells. It identified that, cells, UVB increased intracellular reactive oxygen species (ROS) induced cell apoptosis. In addition, activated a dose dependent manner. Surprisingly,...
Posterior capsule opacification (PCO), a complication of extracapsular lens extraction surgery that causes visual impairment, is characterized by aberrant proliferation and epithelial-mesenchymal transition (EMT) epithelial cells (LECs). Curcumin, exerting inhibitive effects on cell EMT in cancer, serves as possible antidote towards PCO.Cellular LECs after treatment curcumin was measured with MTT assay flow cytometry. The transcriptional expressional levels proteins related to were...
Mechanical pressure plays an important role in many physiological and pathological processes.Mimicking the mechanical present vitro is necessary for related research, but usually requires expensive complicated equipment.In this study we created a simple culture system based on transwell system.By cutting off top rim of insert, cells were compressed between insert membrane well fl oor.The new was proven eff ective that it induced cell morphological change, integrin β1 upregulation, actin...
Covalent CDK7 inhibitor THZ1 is a newly discovered anti-tumor drug.THZ1 affects the function of transcription factor TFIIH by inhibiting CDK7, which in turn RNA polymerase II, and ultimately initiation. Study found that could inhibit proliferation promote apoptosis several tumor cell lines. However, there no report potential side effect normal tissues. In course cancer, muscle consumption cachexia needs to be supplemented differentiation cells. on myogenic remains unclear. Our study this...
To assess the cellular and molecular effects of lidocaine on muscles/myoblasts.Cultured myogenic precursor (C2C12) cells were treated with varying concentrations lidocaine.Cell viability C2C12 was inhibited by in a concentration-dependent manner, ≥0.08%, producing dramatic reduction cell viability. These ≥0.08% arrested cycles G0/G1 phase. Moreover, migration processes at low concentrations. Results from QRT-PCR assays revealed that following treatment lidocaine, Notch1, Notch2, Hes1, Csl...