Abstract Osteoarthritis (OA) is a degenerative joint disorder causing pain and functional disability. Emerging evidence reveals that circular RNAs (circRNAs) play essential roles in OA progression development. This study aimed to investigate the role of novel circRNA factor, circFOXO3, elucidate its underlying molecular mechanism. The function circFOXO3 interaction between downstream mRNA target, forkhead box O3 (FOXO3), were evaluated by western blot (WB), immunofluorescence (IF), RNA...

Abstract Orthopedic prostheses are the ultimate therapeutic solution for various end‐stage orthopedic conditions. However, aseptic loosening and pyogenic infections remain as primary complications associated with these devices. In this study, a hierarchical titanium dioxide (TiO 2 ) nanotube drug delivery system loaded cinnamaldehyde surface modification of implants, is constructed. These specially designed dual‐layer TiO nanotubes enhance material reactivity provide an extensive...

Total temporomandibular joint (TMJ) prosthesis is an effective and reliable method of reconstruction. However, there still urgent need to design a new TMJ because no commercially available appropriate for the clinical application on Chinese population. This study was introduced prospectively confirm safety effectiveness with customized 3D printing additive fabrication in application.Patients unilateral end-stage osteoarthrosis were recruited this from Nov 2016 Mar 2017. Computed tomography...

Rationale: Inflammatory macrophages and osteoclasts (OCs) play critical roles in joint inflammation, which feature the excessive production of reactive oxygen species (ROS), resulting synovial inflammation bone erosion.Scavenging ROS, especially by modulating mitochondrial metabolic activity, could be a desirable strategy for management inflammatory joints.This study aimed to develop mitochondria-targeted supramolecular drug delivery system with exogenous endogenous ROS-scavenging activities...

Implant-associated infection and inflammation are the main causes of implant failure, causing irreversible damage significantly increasing clinical risks economic losses. In this study, a 3D multifunctional architecture is constructed that consisted hierarchical TiO2 nanotubes (NTs) electrospun polyvinylidene fluoride nanofiber layers on surface titanium implant. The movement bacteria through layer facilitated by its appropriate pore sizes electrostatic interactions to reach NT where killed...

Abstract Background Previous research has revealed that the 18 glycoside hydrolase gene family (GH18) member Chitinase 3-like 1 (Chi3l1) can regulate osteoclast differentiation and bone resorption. However, its downstream receptors molecular mechanisms during osteoclastogenesis have yet to be elucidated. Methods Initially, we conducted a comprehensive investigation evaluate effects of recombinant Chi3l1 protein or siRNA on RANKL-induced MAPK/AKT signaling pathways. Moreover, used...

Abstract Background Smart nanoscale drug delivery systems that target acidic tumor microenvironments (TME) could offer controlled release of drugs and modulate the hypoxic TME to enhance cancer therapy. The majority previously reported MnO 2 nanostructures are nanoparticles, nanosheets, or nanocomposites incorporated with other types which may not most effective method for loading therapeutic payloads. Previous studies have designed nanoshells achieve tumor-specific enhanced combination...

Temporomandibular joint osteoarthritis (TMJOA) is a common degenerative disease that causes chronic pain and dysfunction. However, the current understanding of TMJOA pathogenesis limited necessitates further research. Animal models are crucial for investigating due to scarcity clinical samples. Class II malocclusion an occlusal type highly associated with TMJOA, but it currently lacks appropriate animal simulating this in Therefore, study develops new model using unilateral anterior large...

Rationale: Inflammatory osteolysis, characterized by abundant immune cell infiltration and osteoclast (OC) formation, is a common complication induced bacterial products and/or wear particles at the bone-prosthesis interface that severely reduces long-term stability after implantation.Molecular nanoclusters are ultrasmall with unique physicochemical biological properties have great potential as theranostic agents for treating inflammatory diseases.Methods: In this study, heterometallic PtAu2...

Abstract Background The COVID-19 pandemic has become a huge threat to human health, infecting millions of people worldwide and causing enormous economic losses. Many novel small molecule drugs have been developed treat patients with COVID-19, including Paxlovid, which block the synthesis virus-related proteins replication viral RNA, respectively. Despite satisfactory clinical trial results, attention is now being paid long-term side effects these antiviral on musculoskeletal system. To date,...

Low back pain is usually caused by intervertebral disc degeneration (IVDD), during which the involvement of oxidation system imbalance and inflammasome activation cannot be neglected. In this study, we aimed to validate expression level TXNIP in IVDD investigate function potential mechanism action verapamil. upregulated degenerate nucleus pulposus both humans rats, as well tert-butyl hydroperoxide (TBHP)-stimulated cells. Administration verapamil, a classic clinical drug, mitigated...

Excessive bone resorption due to increased inflammatory factors is a common feature of lytic diseases. This group diseases effectively treated with drugs. In recent years, many studies have reported that traditional Chinese medicine herbs substantial effects on inflammation, osteoclast differentiation and maturation, destruction. Herein, we investigated the osthole (OST) lipopolysaccharide- (LPS-) induced macrophage polarization, responses, osteolysis. vitro, used immunofluorescence...

As a degenerative disease in joints, temporomandibular joint osteoarthritis (TMJOA) is characterized by progressive cartilage degradation, subchondral bone remodeling, and chronic synovitis, severely undermining functions quality of life patients. NADPH oxidase 4 (NOX4) contributes to reactive oxygen species (ROS) production inflammatory pathway activation osteoarthritis, which has attracted increasing attention research recent years. GLX351322 (GLX), novel NOX4 inhibitor, exerts protective...

Abstract A series of osteolytic bone diseases are usually related to excessive resorption and osteoclast formation. Thus, agents or drugs which can target development attenuate loss potentially considerable in preventing treating lytic diseases. In recent years, many studies have reported that Notch signaling has substantial impacts on the process differentiation, maturation, destruction. present study, we showed LY411575, a γ‐secretase inhibitor, could potently suppress osteoclast‐specific...

The improvement of Ag nanoparticles (AgNPs), in particular, loaded titania nanotubes, includes not only the antibacterial effect but also balancing side effects from and osteogenesis properties, which can lead to an increased success rate implants. Herein, based on various needs graft inhibit bacteria at different stages vivo, we used a special osteogenic honeycomb-like "large tube over small tube" double-layered nanotube structure created ultra-small-sized silver uniformly surface interior...

Abstract Temporomandibular joint osteoarthritis (TMJOA) is a commonly encountered degenerative disease in oral and maxillofacial surgery. Recent studies have shown that the excessive unbalanced activation of Wnt/β‐catenin signaling connected with pathogenesis TMJOA due to inability inhibit over‐activated Wnt pathway, while Wnt16‐deficient mice has more severe Knee OA. However, efficacy direct intra‐TMJ injection Wnt16 for relief still not directly confirmed. Moreover, small‐molecule drugs...

Abstract The purpose of this study was to confirm the 5-year efficiency temporomandibular joint (TMJ) anchorage, using clinical evaluation and magnetic resonance imaging (MRI). We also studied influence disc length position on postoperative condylar height. Sixty-one patients (76 joints) undergoing TMJ anchorage were followed up for >5 years. Visual analogue scale (VAS) score maximum mouth-opening pre-and postsurgery analysed patient satisfaction recorded. Disc length, condyle height pre-...

Current treatments of osteoarthritis, such as oral medication and intra-articular injections, only provided temporary relief from pain achieved limited advance in inhibiting progression. The development new is hindered by the complicated unclear pathological mechanisms. Oxidative stress immune inflammation are believed to be important factors induction progression osteoarthritis. Herein, this work presents a bioactive material strategy treat based on FPSOH matrixgel with robust...