Parkinson's disease (PD) is a neurodegenerative disorder characterized by motor and nonmotor symptoms due to the selective loss of midbrain dopaminergic neurons. The evidence for chronic inflammatory reaction mediated microglial cells in brain particularly strong PD. In our previous study, we have shown that brain-specific microRNA-124 (miR-124) significantly down-regulated 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model PD it can also inhibit neuroinflammation during...

Abstract The role of microglial-mediated sustained neuroinflammation in the onset and progression Parkinson’s disease (PD) is well established, but mechanisms contributing to microglial activation remain unclear. LincRNA-p21, a studied long intergenic noncoding RNA (lincRNA), plays pivotal roles diverse biological processes diseases. Its inflammation-induced neurotoxicity, however, has not yet been fully elucidated. Here, we report that lincRNA-p21 promotes through p53-dependent...

Human bone marrow-derived mesenchymal stem cells (HBMSCs) are characterized by multiple differentiation potential and potent self-renewal ability, yet much remains to be elucidated on what determines these properties. Long-chain noncoding RNAs (lncRNAs) have been suggested involved in biological processes under physiological pathological conditions, including osteogenic differentiation. Alkaline phosphatase (ALP) activity assay, ALP staining, Alizarin Red Staining were used for detection....

Abstract Periodontal ligament stem cells (PDLSCs) are characterized by multiple differentiation potential and potent self‐renewal ability, yet much remains to be elucidated that what determines these properties. Long noncoding RNAs (lncRNAs) have been suggested involve in biological process under physiological pathological conditions, including osteogenic differentiation. In the present study, we performed comprehensive lncRNA profiling microarray analysis identified prostate...

The expression of major histocompatibility complex class I (MHC-I), a key antigen-presenting protein, can be induced in dopaminergic neurons the substantia nigra, thus indicating its possible involvement occurrence and development Parkinson's disease. However, it remains unclear whether oxidative stress induces disease through MHC-I pathway. In present study, polymerase chain reaction western blot assays were used to determine 1-methyl-4-phenylpyridinium (MPP⁺)-treated SH-SY5Y...

Tongue squamous cell carcinoma (TSCC), the most common epithelial cancer identified in oral cavity, has become one of malignancies across developing countries.Increasing evidence indicates that long non-coding RNAs (lncRNAs) serve as important regulators biology.The focally amplified RNA (FALEC) was found downregulated tissues tongue (TSCC) and predicted to present a good prognosis by bioinformatics analysis.Experiments indicated FALEC knockdown significantly increased proliferation...

Abstract Background Chemokine (C-C motif) ligand 18 (CCL18) affects the malignant progression of varying cancers by activating chemokine receptors. Our previous work has shown that CCL18 promotes hyperplasia and invasiveness oral cancer cells; however, cognate receptors involved in pathogenesis squamous cell carcinoma (OSCC) have not yet been identified. This study aimed to investigate molecular mechanisms which underlie promotive effects on OSCC binding functional Methods The expression...

Abstract Background Tongue squamous cell carcinoma (TSCC) is one of the most common oral tumors. Recently, long intergenic noncoding RNA 00958 (LINC00958) has been identified as an oncogene in human cancers. Nevertheless, role LINC00958 and its downstream mechanisms TSCC still unknown. Methods The effect on cells proliferation growth were assessed by CCK-8, colony formation, 5-Ethynyl-2′-deoxyuridline (EdU) assay flow cytometry assays vitro tumor xenograft model vivo. Bioinformatics analysis...

Bone marrow-derived mesenchymal stem cells (BM-MSCs) implantation shows a repair effect on erectile function in diabetes mellitus-induced dysfunction (DMED) due to its differentiative capacity into endothelial (ECs) that contributes repair. This study was designed explore the functional role and mechanism of long noncoding RNA (lncRNA)-metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) BM-MSCs-mediated DMED repairing. The rat model established evaluated by calculating...

Mesenchymal stem cells (MSCs) are bone marrow stromal capable of differentiating into different tissue types. Osteoblastic differentiation is a complex process that critical for formation. An increasing number studies have suggested microRNAs (miRNAs) may serve important roles in various biological processes, including osteogenesis MSCs. However, less known about the participation particular miRNAs osteogenic adipose-derived (ADSCs). In order to identify functional and key genes involved...

MicroRNA (miR)-99a-5p acts as a tumor suppressor in several tumors, including bladder cancer and breast cancer, but its biological function oral squamous cell carcinoma (OSCC) is poorly understood.miR-99a-5p expression was determined OSCC tissues lines using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Cell proliferation assessed by the Counting Kit-8 assay colony formation assay. Wound healing Transwell assays were used to analyze migration invasion abilities,...

Mutation of the telomerase reverse transcriptase (TERT) promoter has been demonstrated as an unfavorable prognostic marker in patients with isocitrate dehydrogenase wild-type (IDHwt) glioma. This study aimed to investigate immune role TERT mutation status which could improve prediction IDHwt. status, IDH mutation, and 1p-19q codeletion data were obtained from 614 glioma cases Cancer Genome Atlas, 325 Chinese Glioma Atlas. The same information was 49 clinical tissues. is preferentially...

Objective . Long noncoding RNAs (lncRNAs) have been demonstrated to regulate many biological processes including differentiation. However, their role in osteogenic differentiation was poorly known. Materials and Methods In this study, we first globally profiled the differentially expressed lncRNAs mRNAs during of human bone marrow mesenchymal stem cells (hBMMSCs). Bioinformatics analysis performed further analyze these significantly changed molecules. Then lncRNA ENST00000502125.2...

Adipose-derived stem cells (ADSCs) are increasingly used in regenerative medicine because of their potential to differentiate into multiple cell types, including osteogenic lineages. Sirtuin protein 6 (SIRT6) is a nicotinamide adenine dinucleotide (NAD)-dependent deacetylase that plays important roles differentiation. NOTCH signaling has also been reported involve However, the function SIRT6 differentiation ADSCs and its relation pathways yet be explored.The vitro study with human (hADSCs)...

The pathogenesis of Parkinson’s disease remains unclear that there is no cure for yet. abnormal expressions certain miRNA are closely related to the occurrence and progression disease. Here, we demonstrate miR-9-5p inhibits dopaminergic neuron apoptosis via regulation β-catenin signaling which directly targets SCRIB, a tumor suppressor gene. Besides, improved motor function mice with results this study suggest might be potential therapeutic target against

Background LncRNA PCAT29 has been reported to play a role in the development of cancer; FLOT1 renal carcinoma also identified. This study aimed at exploring interaction between them and their influence on progression carcinoma. M ethods The expression levels lncRNA protein tissues were determined separately using qRT-PCR western blot, respectively. endogenous genes was modulated by recombinant plasmid cell transfection. viability, invasion, migration detected MTT assay, transwell...

The aim of the present study was to identify agents capable inhibiting invasion and metastasis tongue squamous cell carcinoma thereby improve outcomes patients suffering from cancer. FRMD4A antibodies were used probe 78 paraffin-embedded specimens 15 normal tissues, which served as controls. Immunohistochemical methods then for analysis. Clinical pathological parameters obtained, association between expression in samples analyzed. human cancer line CAL27 effects FRMD4A. cells transfected...

Regional and distant metastases are the principal reasons underlying high mortality rate associated with tongue squamous cell carcinoma (TSCC); however, precise molecular mechanisms involved in tumorigenesis remain unknown. The present study aimed to determine expression mechanism of regulation Wnt7a growth metastasis TSCC. mRNA protein levels were examined TSCC tissues using reverse transcription‑quantitative polymerase chain reaction immunohistochemical staining. A loss‑of‑function assay...

Long non‑coding RNAs (lncRNAs) are a specific group of RNA molecules that do not encode proteins. They have been shown to serve important regulatory functions in various biological and cell differentiation processes. However, the potential mechanisms lncRNAs associated with osteogenic human bone marrow mesenchymal stem cells (hBMSCs) remain be elucidated. The present study aimed investigate differentially expressed during hBMSCs, along those lncRNAs. To this end, three groups hBMSCs were...

Abstract: The aim of this work was to investigate the role chemokines in proliferation and migration tongue squamous cell carcinoma (TSCC). Out 80 cytokines surveyed by a human cytokine antibody array, three chemokines, macrophage inflammatory protein-3α (MIP-3α), protein-1β (MIP-1β), interferon gamma-induced protein 10 (IP-10), showed elevated expression TSCC cells (CAL-27 UM-1), compared oral mucosal epithelial cells. Immunohistochemistry confirmed high level MIP-3α tissues, especially...

Abstract Background Erectile dysfunction (ED) is a common male sexual dysfunction, with an increasing incidence, and the current treatment often ineffective. Methods Vascular endothelial growth factor (VEGFA) was used to treat bone marrow-derived mesenchymal stem cells (BM-MSCs), their cell migration rates were determined by Transwell assays. The expression of von Willebrand Factor (vWF)VE-cadherin, nitric oxide synthase(eNOS) markers qRT‒PCR Western blot analyses. MALAT1-induced...