Breathomics testing has been considered a promising method for detection and screening lung cancer. This study aimed to identify breath biomarkers of cancer through perioperative dynamic breathomics testing.The discovery was prospectively conducted between Sept 1, 2020 Dec 31, in Peking University People's Hospital China. High-pressure photon ionisation time-of-flight mass spectrometry used before surgery 4 weeks after surgery. 28 volatile organic compounds (VOCs) were selected as candidates...

Non-small cell lung cancer (NSCLC) is the most common type of human cancers, which has diverse pathological features. Although many signaling pathways and therapeutic targets have been defined to play important roles in NSCLC, limiting efficacies achieved.Bioinformatics methods were used identify differential long non-coding RNA expression NSCLC. Real-time RT-PCR experiments examine pattern lncRNA PKMYT1AR, miR-485-5p. Both vitro vivo functional assays performed investigate role...

BackgroundCCN family, comprising six members (Cyr61, CTGF, Nov, WISP-1, WISP-2, WISP-3), is involved in the stimulation of cell proliferation, migration, adhesion, angiogenesis, and tumorigenesis. Several studies have shown that expression Cyr61, WISP-1 affects tumorigenic potential lung cancer cells vitro. However, correlation CCN family proteins clinical features remains unknown.Methodology Principal FindingsIn present work, we quantified mRNA levels samples from 60 primary cancers their...

Abstract The incidence of cutaneous melanoma (CM) has been increasing annually worldwide. In this study, we identify that MrgprF, a MAS related GPR family member, is decreased in tissues and cell lines due to hypermethylation its promoter region, show patients with CM expressing high levels MrgprF exhibit an improved clinical outcome. We demonstrate forced expression inhibits tumor proliferation, migration, xenograft growth, metastasis. On the contrary, knockdown promotes proliferation...

Abstract Background We have reported previously that overexpression of glucose-regulated protein 78 (GRP78) promotes the invasion hepatocellular carcinoma. However, whether GRP78 knockdown affects extracellular matrix degradation has not been elucidated. Here we are going to determine affect ECM and role MMP-2 MMP-9 in these process carcinoma cells. Methods Human cell line SMMC7721 HepG2 were cultured DMEM supplemented with 10% FBS, RT-PCR western blot used detect endogenous expression...

Abstract Background Little is known about the relationship between N6-methyladenosine (m6A)-related genes and tumor immune microenvironment (TIME) in non-small cell lung cancer (NSCLC). It unclear which m6A regulators are essential for NSCLC progression. The aim of this work was to excavate role m6A-related TIME progression NSCLC. Methods Based on bioinformatics analysis, heterogeneous nuclear ribonucleoprotein C (HNRNPC) considered as most influential regulator. Further study investigated...

Background: There is a rapid increase in lung adenocarcinomas (LUAD), and studies suggest associations between cuproptosis the occurrence of various types tumors. However, it remains unclear whether plays role LUAD prognosis. Methods: Dataset TCGA-LUAD was treated as training cohort, while validation cohort consisted merged datasets GSE29013, GSE30219, GSE31210, GSE37745, GSE50081. Ten studied cuproptosis-related genes (CRG) were used to generated CRG clusters cluster-related differential...

Invasion is a major characteristic of hepatocellular carcinoma and one the main causes refractory to treatment. We have previously reported that GRP78 promotes invasion although mechanism underlying this change remains uncertain. In paper, we explored role cell surface in regulation cancer cells. found neutralization endogenous with anti-GRP78 antibody inhibited adhesion lines Mahlavu SMMC7721. However, forced expression facilitated further demonstrated inhibition specifically secretion...

Emerging data have suggested that cell surface GRP78 is a multifunctional receptor and has been linked to proliferative antiapoptotic signaling cascades. Activated α2-macroglobin (α2M*) natural circulating ligand of GRP78. Association with α2M* involved in the regulation proliferation, survival apoptosis human cancers.The invasion metastasis HCC cells were examined using transwell wound healing assay; Cell expression was detected by western assay. Translocation from cytosol observed...

The aim of this study was to discover and identify potential protein biomarkers specific for non-small cell lung cancer (NSCLC). Two hundred thirty five (235) Serum samples with 112 NSCLC 123 controls were randomly divided into a training set blind testing set. proteomic profiles analyzed using surface-enhanced laser desorption/ionization time-of-flight mass spectroscopy (SELDI-TOF-MS). Candidate purified by high Performance Liquid Chromatography (HPLC) identified liquid chromatography...

// Yan-jiao Gu 1,* , Hong-dan Li 2,* Liang Zhao 2 Song 3 Wu-bin He 1 Rui Chang Su 4 Hua-chuan Zheng 5 and Rong-jian Pathology Department, The First Affiliated Hospital of Liaoning Medical College, Jinzhou, China Central Laboratory, Pharmacy Veterinary Medicine Cancer Research Center, Key Laboratory Brain Spinal Cord Injury Province Animal * These authors have contributed equally to this work Correspondence to: Su, email: Keywords : GRP78, chemoresistance, 5-FU, LSF, TS Received April 16,...

<h3>Objective:</h3> L1 cell adhesion molecule (L1CAM) exhibits oncogenic activity in tumors. However, the link between L1CAM and tumor microenvironment remains poorly understood patients with esophageal squamous carcinoma (ESCC). In this study, we investigated how expression ESCC affects characteristics of cells microenvironment. <h3>Methods:</h3> Human samples were collected, mRNA protein levels examined by real-time PCR immunohistochemistry. Overexpression knockdown gene assays used for...

A triage test is needed to increase the detection rate for esophageal cancer.To investigate whether breathomics can detect cancer among patients without a previous diagnosis of using high-pressure photon ionization time-of-flight mass spectrometry (HPPI-TOFMS).This diagnostic study included participants who planned receive an upper endoscopy or surgery esophagus at single center in China. Exhaled breath was collected with self-designed collector and air bags before underwent these...

This study aims to investigate the influence of glucose regulated protein (GRP) 78 on osteoblast differentiation in periodontal ligament fibroblasts (PDLFs) under cyclic mechanical stretch and determine underlying mechanism.

Calcyphosine (CAPS), a calcium-binding protein, has been identified as potential diagnostic and prognostic biomarker in several human carcinomas. However, little is known about CAPS esophageal squamous cell carcinoma (ESCC). The present study aimed to investigate the expression levels of ESCC tissues evaluate its clinicopathological significance. Reverse transcription-quantitative polymerase chain reaction immunohistochemical staining were conducted detect adjacent non-cancerous tissues....

Lung adenocarcinoma (LUAD) is the leading histological subtype of lung cancer worldwide, causing high mortality each year. The tumor immune cell infiltration (ICI) closely associated with clinical outcome LUAD patients. present study was designed to construct a gene signature based on ICI predict prognosis.Downloaded raw data three cohorts TCGA-LUAD, GSE72094, and GSE68465 treat them as training cohort, validation cohort one, two for this research. Unsupervised clustering detailed grouped...

The majority of pregnant women present an increase in lipids. To investigate the influence non-fasting state lipid and lipoprotein profile pregnancy, we have aimed to assess dynamic change serum with glucose pregnancy contrast differences between fasting state. Forty-five 41 controls were included our study. All samples assayed for TC, TG, HDL-C, LDL-C, ApoB, ApoA-1, Lp(a), sdLDL, Glu concentrations. comparison (fasting 2 h after breakfast), these measurement results at three point-in-time,...

Melanoma-associated antigens (MAGEs) are a group of well-characterized members the cancer/testis antigen family, which expressed in variety malignant tumors. MAGE-A9, subfamily MAGE-As, has been studied number types cancer and have associated with unfavorable survival outcome. However, expression MAGE-A9 human esophageal squamous cell carcinoma (ESCC) association clinicopathological characteristics ESCC, particularly prognostic characteristics, remains unknown. The present study aimed at...

Background The extract of Ginkgo biloba leaves tablets, ginaton, is widely used in treating ischemic cerebrovascular disease the clinic. This study aimed to investigate expression aquaporin-1 (AQP-1) rat lung with ischemia/ reperfusion injury after pretreatment and whether ginaton reduces acute caused by ischemia/reperfusion injury. Methods Adult Wistar rats were divided into two groups. Some as donors ( n =20), others recipients =20). Left lungs donor for isolated model, which perfused only...