A5101971748- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- MicroRNA in disease regulation
- 3D Printing in Biomedical Research
- Reproductive Biology and Fertility
- RNA Interference and Gene Delivery
- Animal Genetics and Reproduction
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer-related Molecular Pathways
- Bone health and osteoporosis research
- Angiogenesis and VEGF in Cancer
- melanin and skin pigmentation
- Bone Metabolism and Diseases
- Molecular Sensors and Ion Detection
- Renal and related cancers
- Protein Tyrosine Phosphatases
- Viral Infectious Diseases and Gene Expression in Insects
- Cardiac Valve Diseases and Treatments
- Circular RNAs in diseases
- Higher Education and Teaching Methods
- NF-κB Signaling Pathways
- Antimicrobial Resistance in Staphylococcus
- Electrohydrodynamics and Fluid Dynamics
- Bioinformatics and Genomic Networks
The First Affiliated Hospital, Sun Yat-sen University
2014-2024
Zhongshan Hospital
2014-2024
Fudan University
2014-2024
Sun Yat-sen University
2014-2024
Shanghai Institute of Hematology
2019-2021
Zhujiang Hospital
2020
Southern Medical University
2020
University of California, Davis
2020
Binzhou Medical University
2019
Northeast Forestry University
2015-2017
Summary Introduction To investigate the cardioprotective effect of Micro RNA ‐21 (miR‐21) in murine myocardial infarction ( MI ). Methods Forty C57 BL /6 male mice were divided into sham group, LV ‐ GFP and miR‐21 group. Mice group subjected to by left anterior descending artery LAD ) ligation, while chest was opened/closed without ligation In expression area its surrounding areas detected at 1st, 2nd, 4th week after experiment. Subsequently, lentivirus expressing that did not express...
Until recently, it has been difficult to derive and maintain stable embryonic stem cells lines from livestock species. Sheep ESCs with characteristics similar those described for rodents primates have not produced. We report the derivation of sheep under a chemically defined culture system containing fibroblast growth factor 2 (FGF2) tankyrase/Wnt inhibitor (IWR1). also show that several conditions used stabilizing naïve intermediate pluripotency states in humans mice were unsuitable ovine...
Induced pluripotent stem cells (iPSCs) have attracted keen interest in regenerative medicine. The generation of iPSCs from somatic can be achieved by the delivery defined transcription factor (Oct4, Sox2, Klf4, and c-Myc[OSKM]). However, most instances iPSC-generation been potentially harmful genome-integrating viral vectors. Here we report mouse embryonic fibroblasts (MEFs) using arginine-terminated 4 polyamidoamine (G4Arg) nanoparticles as a nonviral transfection vector for single plasmid...
Utilizing oxidative stress has recently been regarded as a potential strategy for tumor therapy. The NUAK family SNF1-like kinase 1 (NUAK1) is critical component of the antioxidant defense system and necessary survival tumors. Therefore, NUAK1 considered an attractive therapeutic target in cancer. However, therapy induced elevated ROS levels to activate Unc-51-like (ULK1) pathway promote protective autophagy ULK1-dependent mitophagy. Thus, combined inhibition ULK1 showed strong synergistic...
Abstract: Mesenchymal stem cell (MSC) transplantation has attracted much attention in myocardial infarction therapy. One of the limitations is poor survival grafted cells ischemic microenvironment. Small interfering RNA-mediated prolyl hydroxylase domain protein 2 (PHD2) silencing MSCs holds tremendous potential to enhance their and paracrine effect after transplantation. However, an efficient biocompatible PHD2 system for clinical application lacking. Herein, we developed a novel based on...
Both microRNAs (miRNAs) and endogenous small interfering RNAs (endo-siRNAs) play key regulatory roles in gene expression. Some studies have demonstrated that the function of miRNA is suppressed mouse oocytes, suggesting endo-siRNA, not miRNA, essential for female meiosis. This finding has yet to be confirmed other species. In this study, by knockdown DICER1, DROSHA its cofactor DiGeorge syndrome critical region 8 (DGCR8) porcine we found proportion oocytes with DICER1 deficiency developed...
Patients with bicuspid aortic valve (BAV) are at increased risk for ascending dilation (AAD). Our study was aimed systemically analyzing the expression profile and mechanism of circulating plasma exosomal microRNAs (miRNAs) related to BAV AAD.
Microarray data of osteoporosis (OP) were analyzed based on prediction transcription factors (TFs) or their targets as well influences TFs TF network to uncover key in OP. The microarray E-GEOD-35956 was downloaded from the GPL570 platform. Differentially expressed genes (DEGs) with logarithm fold change (|logFC|) >2 and P-value <0.05 identified between OP samples normal controls. screened DEGs ITFP, Marbach 2016, TRRUST databases. enriched using Fisher's exact test. selected impact factors....
Cardiomyocyte differentiation is a multi-step process which involves number of signalling pathways. microRNAs exhibit regulatory functions in various diseases and are involved the pathways multiple physiological processes, but specific particular mRNAs often not fully understood. an example this that role miR-590-3p cardiomyocytes remains unclear. In current study, RT-qPCR was used to determine expression differentiated from embryonic carcinoma cell line P19CL6. MTT, EdU, caspase-3 activity...
Aortic aneurysm is a common cardiovascular disease characterised by continuous dilation of the aorta, and this places heavy burden on healthcare worldwide. Few drugs have been suggested to be effective in controlling progression aortic aneurysms. Preclinical drug responses from traditional cell culture animals are usually controversial. An vitro model great demand for successful screening. In study, we induced an microphysiological system test metformin, which potential treatment Human...
Differentiated cell nuclei can be reprogrammed to a pluripotent state in several ways, including incubation with oocyte extracts, transfer into enucleated oocytes, and induced stem technology. Nuclear transfer-mediated reprogramming has been proven the most efficient method. Maternal factors stored oocytes have critical roles on nuclear early embryo development, but remain elusive.In this study, we showed of porcine became matured at 33 h IVM rate had no significant difference 42 (p > 0.05)....
Abiotrophia (ABI) and Granulicatella (GRA) are rare causative pathogens in infective endocarditis (IE). This study aims to describe the epidemiology, clinical characteristics, outcome of ABI/GRA-IE. The main features ABI/GRA-IE were compared with Viridans group streptococci (VGS) IE.