Abstract Heart failure (HF) is a frequent consequence of myocardial infarction (MI). Identification the precise, time-dependent composition inflammatory cells may provide clues for establishment new biomarkers and therapeutic approaches targeting post-MI HF. Here, we investigate spatiotemporal dynamics MI-associated immune in mouse model MI using spatial transcriptomics single-cell RNA-sequencing (scRNA-seq). We identify twelve major cell populations; their proportions dynamically change...

Silicon monoxide (SiO), which exhibits better cyclability compared to silicon while delivering higher capacity than that of graphite, is an adequate material for the development lithium-ion batteries (LIBs) having energy densities. However, incorporating silicon-based materials including SiO into stable graphite anode inevitably degrades not only cycle life but also calendar LIBs, little known about their aging mechanisms. Here, SiO-induced thermal instability graphite/SiO composite...

Abstract Predicting the risk of metastasis before starting prostate cancer (PCa) treatment can minimize overtreatment indolent cases and help choosing appropriate treatment. The levels circulating microRNAs (miRNAs) from body fluids be used as noninvasive prognostic biomarkers. In this study, urinary exosomal miRNA expression profiles 149 PCas were determined miRNAs associated with identified: miR-21, miR-16, miR-142-3p, miR-451, miR-636. When evaluating clinical factors together, miR-636,...

Vulvar squamous cell carcinoma (SCC) consists of two different etiologic categories: human papilloma virus (HPV)-associated (HPV (+)) and HPV-non-associated (−)). There have been no genome-wide studies on the genetic alterations vulvar SCCs or differences between HPV (+) (−) SCCs. In this study, we performed whole-exome sequencing copy number profiling 6 9 found known mutations (TP53, CDKN2A HRAS) (CNAs) (7p 8q gains 2q loss) in (+), novel PIK3CA, BRCA2 FBXW7 that had not reported exhibited...

Abstract Endometriosis consists of ectopic endometrial epithelial cells (EEECs) and stromal (EESCs) mixed with heterogeneous cells. To address how endometriosis‐constituting are different from normal endometrium among endometriosis subtypes their molecular signatures related to phenotypic manifestations, we analyzed ovarian cyst (OEC), superficial peritoneal (SPE), deep infiltrating (DIE) 12 patients using single‐cell RNA‐sequencing (scRNA‐seq). We identified 11 cell clusters, including...

Although high grade prostatic intraepithelial neoplasia (HGPIN) is considered a neoplastic lesion that precedes prostate cancer (PCA), the genomic structures of HGPIN remain unknown.Identification landscape and differences between PCA may drive progression to PCA.We analyzed 20 regions paired from six patients using whole-exome sequencing array-comparative hybridization.Somatic mutation copy number alteration (CNA) profiles were measured compared.The total mutations CNAs HGPINs significantly...

The mechanism underlying the progression of actinic keratosis (AK) and cutaneous squamous cell carcinoma (SCC) in situ (SCCIS) to SCC remains unclear. To investigate this, we performed regional microdissection targeted deep sequencing (n = 10) paired adjacent sun-damaged epidermis (SE)/AK/SCCIS 13) samples detect mutations copy number alterations. Most (11/13) SE/AK/SCCIS tissues harbored ≥1 driver alterations, indicating their precancerous nature. All pairs except one showed genome...

Although PD-1 blockade is effective for treating several types of cancer, the efficacy this agent in glioblastoma largely limited. To overcome non-responders and immunosuppressive tumor microenvironment, combinational immunotherapeutic strategies with anti-PD-1 need to be considered. Here, we developed IL-12-secreting mesenchymal stem cells (MSC_IL-12) tropism evaluated therapeutic effects anti-PD-1, MSC_IL-12, their combination against glioblastoma. Therapeutic responses were using an...

Abstract The overexpression of trefoil factor family 3 (TFF3) is observed in a variety cancers, including prostate cancer (PCa), and its potential role carcinogenesis, such as activating the PI3K/AKT pathway, suggested. However, related mechanisms tumorigenesis remain unknown. To elucidate TFF3 PCa, we silenced two PCa cell lines that overexpressed explored molecular mechanism behind antiapoptotic role. We also examined expression 108 Korean specimens 106 normal tissues by...

The development of an in vitro three-dimensional (3D) culture system with cryopreserved biospecimens could accelerate experimental research screening anticancer drugs, potentially reducing costs and time bench-to-beside. However, minimal has explored the application 3D bioprinting-based cancer models to derived from patients advanced melanoma. We investigated whether 3D-printed collagen scaffolds enable propagation maintenance patient-derived melanoma explants (PDMEs). were fabricated a 3DX...

Avian influenza (AIV) outbreaks can induce fatal human pulmonary infections in addition to economic losses the poultry industry. In this study, we aimed develop a rapid and sensitive point-of-care AIV test using loop-mediated isothermal amplification (LAMP) technology. We designed three sets of reverse transcription LAMP (RT-LAMP) primers targeting matrix (M) hemagglutinin (HA) genes H5 H9 subtypes. RT-LAMP universal M gene was screen for presence assays H5-HA H9-HA were discriminate between...

Salmonella species are among the major pathogens that cause foodborne illness outbreaks. In this study, we aimed to develop a loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive detection of species. We designed LAMP primers targeting hilA gene as universal marker A total seven strains 11 non-Salmonella pathogen from eight different genera were used in study. All showed positive signals with assay; however, there was no non-specific signal strains. The limit 100...

Many studies have demonstrated the mechanisms of progression to castration-resistant prostate cancer (CRPC) and novel strategies for its treatment. Despite these advances, molecular underlying CRPC remain unclear, currently, no effective treatments are available. Here, we characterized key genes involved in gain insight into potential therapeutic targets. Bicalutamide-resistant cells derived from LNCaP were generated named Bical R. RNA sequencing was used identify differentially expressed...

In many epithelial tissues, the Cl(-) efflux via Ca(2+)-activated channels (Cl(Ca)) play a key role for fluid secretion. To elucidate mechanism of prostatic secretion, properties whole-cell chloride conductance were investigated.Rat prostate secretory cells (RPSECs) isolated by collagenase treatment, and used voltage clamp. Both extra- intracellular monovalent cations replaced N-methyl-D-glucamate to record current selectively.A bath application Ca(2+)-ionophore, ionomycin (0.2 micro M),...

OBJECTIVE To characterize the voltage‐activated ion‐channel currents in guinea‐pig prostate smooth muscle cells (GPSMCs). MATERIALS AND METHODS GPSMCs were isolated using collagenase, and used a whole‐cell patch clamp study. RESULTS When dialysed with CsCl solution all outward K + blocked step‐like depolarization (holding voltage − 70 mV) of cell membrane evoked inward that completely by nifedipine (1 µmol/L). With KCl solution, step depolarizations showed composed fast, transient current (I...

Troponin C type 1 (TNNC1) is commonly overexpressed in ovarian cancer. However, the biological implications of TNNC1 overexpression on cancer malignization and its related mechanism remain unknown. To elucidate these implications, we knocked out gene TNNC1-overexpressing SKOV-3-13 cells using CRISPR/Cas-9 technology observed changes metastatic phenotypes molecular pathways. TNNC1-knockout (KO) showed significantly reduced proliferation colony formation when compared with wild-type (P <...

Robust identification of genetic alterations is important for the diagnosis and subsequent treatment tumors. Screening using tumor tissue samples may lead to biased interpretations because heterogeneous nature mass. Liquid biopsy has been suggested as an attractive tool non-invasive follow-up cancer outcomes. In this study, we aimed verify whether mutations identified in primary could be consistently detected plasma cell-free DNA (cfDNA) by digital polymerase chain reaction (dPCR). We first...

The prostate gland contains numerous neuroendocrine cells (PNECs) innervated by adrenergic neurons. PNECs are believed to influence the growth and physiological function of via paracrine release hormones.Using fura-2 fluorescence measurement patch-clamp techniques, we investigated effects stimulation on cytosolic concentration Ca2+ ([Ca2+]c) high voltage-activated channel currents (HVA-I(Ca)) putative rat (RPNECs) freshly isolated an enzymic digestion.Noradrenaline (NA, 1 microM) induced a...