Daniel E. Cruz
A5018502312- Metabolomics and Mass Spectrometry Studies
- Nutritional Studies and Diet
- Cardiovascular Health and Risk Factors
- Genetic Associations and Epidemiology
- Particle physics theoretical and experimental studies
- Advanced Proteomics Techniques and Applications
- Diet and metabolism studies
- Bioinformatics and Genomic Networks
- Machine Learning in Bioinformatics
- Click Chemistry and Applications
- Health disparities and outcomes
- High-Energy Particle Collisions Research
- Nutrition, Genetics, and Disease
- Alcohol Consumption and Health Effects
- Particle Accelerators and Free-Electron Lasers
- Soil Geostatistics and Mapping
- Phagocytosis and Immune Regulation
- Angiogenesis and VEGF in Cancer
- Immune cells in cancer
- Muscle metabolism and nutrition
- Cardiac Health and Mental Health
- Reproductive System and Pregnancy
- Cancer-related gene regulation
- Superconducting Materials and Applications
- Liver Disease Diagnosis and Treatment
Beth Israel Deaconess Medical Center
2019-2025
Harvard University
2021-2025
Hadassah Medical Center
2025
Universidade Federal de Viçosa
2024
Boston University
2022-2023
Beth Israel Deaconess Hospital
2020
Johns Hopkins Medicine
2019
Johns Hopkins University
2019
University of California, Los Angeles
1998-2018
Ronald Reagan UCLA Medical Center
2018
High-throughput proteomic profiling using antibody or aptamer-based affinity reagents is used increasingly in human studies. However, direct analyses to address the relative strengths and weaknesses of these platforms are lacking. We assessed findings from SomaScan1.3K ( N = 1301 reagents), SomaScan5K platform 4979 Olink Explore 1472 reagents) techniques 568 adults Jackson Heart Study 219 participants HERITAGE Family across four performance domains: precision, accuracy, analytic breadth,...
Background: Heart failure (HF) is a heterogeneous disease characterized by significant metabolic disturbances; however, the breadth of dysfunction before onset overt not well understood. The purpose this study was to determine association circulating metabolites with incident HF uncover novel pathways disease. Methods: We performed targeted plasma metabolomic profiling in deeply phenotyped group Black adults from JHS (Jackson Study; n=2199). related associated established etiological...
Abstract Integrating genetic information with metabolomics has provided new insights into genes affecting human metabolism. However, gene-metabolite integration been primarily studied in individuals of European Ancestry, limiting the opportunity to leverage genomic diversity for discovery. In addition, these analyses have principally involved known metabolites, majority profiled peaks left unannotated. Here, we perform a whole genome association study 2,291 metabolite (known and unknown...
Plasma proteins are critical mediators of cardiovascular processes and the targets many drugs. Previous efforts to characterize genetic architecture plasma proteome have been limited by a focus on individuals European descent leveraged genotyping arrays imputation. Here we describe whole genome sequence analysis in with greater African ancestry, increasing our power identify novel determinants.
Measures from affinity-proteomics platforms often correlate poorly, challenging interpretation of protein associations with genetic variants (pQTL) and phenotypes. Here, we examined 2,157 proteins measured on both SomaScan 7k Olink Explore 3072 across 1,930 participants similarity to European, African, East Asian, Admixed American ancestry references. Inter-platform correlation coefficients for these followed a bimodal distribution (median r=0.30). Protein measures were associated (pQTLs),...
Metabolic responses to exercise training are variable. Metabolite profiling may aid in the clinical assessment of an individual's responsiveness interventions.To investigate association between a novel circulating biomarker hepatic fat, dimethylguanidino valeric acid (DMGV), and metabolic health traits before after 20 weeks endurance training.This study involved cross-sectional longitudinal analyses Health, Risk Factors, Exercise Training, Genetics (HERITAGE) Family Study, 20-week,...
<h3>Importance</h3> African American individuals have disproportionate rates of coronary heart disease (CHD) but lower levels artery calcium (CAC), a marker subclinical CHD, than non-Hispanic White individuals. may distinct metabolite profiles associated with incident CHD risk compared individuals, and examination these differences could highlight important processes that differ between them. <h3>Objectives</h3> To identify novel biomarkers CAC among to replicate findings in multiethnic...
Racial differences in metabolomic profiles may reflect underlying social determinants of health by self-reported race and be related to racial disparities coronary heart disease (CHD) among women the United States. However, magnitude between Black White States has not been well-described. It also remains unknown whether such are CHD risk. Plasma were analyzed using liquid chromatography-tandem mass spectrometry WHI-OS (Women's Health Initiative-Observational Study; 138 696 women), WHI-HT...
The endothelium plays a critical role in promoting inflammation cardiovascular disease and other chronic inflammatory conditions, many small-molecule screens have sought to identify agents that prevent endothelial cell activation. Conversely, an augmented immune response can be protective against microbial pathogens cancer immunotherapy. Yet, induce activation not been reported. In this regard, bioassay was developed identifies activated by its capacity trigger macrophage protein 1 beta from...
The differentiation of intestinal intraepithelial lymphocytes (IEL) remains controversial, which may be due in part to the phenotypic complexity these T cells. We have investigated here development IEL mice on recombination activating gene (RAG)-2−/− background express a cell antigen receptor (TCR) transgene specific for an H-Y peptide presented by Db (H-Y/Db × RAG-2− mice). In contrast thymus, small intestine female H-Y/Db is severely deficient number IEL; TCR transgene+ CD8αα and CD8αβ are...
Brief Summary COVID-19 is one of the most consequential pandemics in last century, yet biological mechanisms that confer disease risk are incompletely understood. Further, heterogeneity outcomes influenced by race, though relative contributions structural/social and genetic factors remain unclear. 1,2 Very recent unpublished work has identified two loci greater for respiratory failure COVID-19: ABO locus 3p21.31 locus. 3 To understand how these might whether this differs we utilized...
Background: Increased left ventricular (LV) mass is associated with adverse cardiovascular events including heart failure (HF). Both increased LV and HF disproportionately affect Black individuals. To understand the underlying mechanisms, we undertook a proteomic screen in cohort compared findings to results from White cohort. Methods: We measured 1305 plasma proteins using SomaScan platform 1772 participants (mean age, 56 years; 62% women) JHS (Jackson Heart Study) assessed by 2-dimensional...
Nontargeted metabolomics methods have increased potential to identify new disease biomarkers, but assessments of the additive information provided in large human cohorts by these less biased techniques are limited. To diversify our knowledge diabetes-associated metabolites, we leveraged a method that measures 305 targeted or “known” and 2,342 nontargeted “unknown” compounds fasting plasma samples from 2,750 participants (315 incident cases) Jackson Heart Study (JHS)—a community cohort...
BACKGROUNDMost GWAS of plasma proteomics have focused on White individuals European ancestry, limiting biological insight from other ancestry-enriched protein quantitative loci (pQTL).METHODSWe conducted a discovery approximately 3,000 proteins measured by the antibody-based Olink platform in 1,054 Black adults Jackson Heart Study (JHS) and validated our findings Multi-Ethnic Atherosclerosis (MESA). The genetic architecture identified pQTLs was further explored through fine mapping admixture...
Scope New biomarkers are needed that representative of dietary intake. Methods and Results We assess metabolites associated with Southern patterns in 1401 Jackson Heart Study participants. Three empirically derived using principal component analysis: meat fast food, fish vegetables, starchy foods. randomly select two subsets the study population: two‐third sample for discovery ( n = 934) one‐third replication 467). Among 327 analyzed, 14 significantly food pattern, four vegetables none foods...
Abstract Severe heart failure is increasingly being managed by cardiac transplantation, and in some cases mechanical support devices serve as destination therapies. Left ventricular assist (LVADs) were approved for therapy end stage patients before the more advanced total artificial modality became available. One common complication of device placement acute kidney injury. Historically, with have had to inpatient hemodialysis until combined transplant. Though, units started accepting LVAD...
N-acyl amino acids are a large family of circulating lipid metabolites that modulate energy expenditure and fat mass in rodents. However, little is known about the regulation potential cardiometabolic functions humans. Here, we analyze phenotype associations genetic four plasma N-fatty acyl (N-oleoyl-leucine, N-oleoyl-phenylalanine, N-oleoyl-serine, N-oleoyl-glycine) 2,351 individuals from Jackson Heart Study. N-oleoyl-leucine N-oleoyl-phenylalanine were positively associated with traits...
N-acyl amino acids are a large family of circulating lipid metabolites that modulate energy expenditure and fat mass in rodents. However, little is known about the regulation potential cardiometabolic functions humans. Here, we analyze phenotype associations genomic four plasma (N-oleoyl-leucine, N-oleoyl-phenylalanine, N-oleoyl-serine, N-oleoyl-glycine) 2351 individuals from Jackson Heart Study. We find levels specific associated with disease endpoints independent free acid patterns...