A5042412393- Ovarian cancer diagnosis and treatment
- Cervical Cancer and HPV Research
- Hepatitis B Virus Studies
- Endometriosis Research and Treatment
- Genital Health and Disease
- Reproductive tract infections research
- Reproductive System and Pregnancy
- Endometrial and Cervical Cancer Treatments
- PARP inhibition in cancer therapy
- Renin-Angiotensin System Studies
- Advanced Electron Microscopy Techniques and Applications
- CRISPR and Genetic Engineering
- Pregnancy and Medication Impact
- Reproductive Biology and Fertility
- Renal and Vascular Pathologies
- Cancer Immunotherapy and Biomarkers
- Cardiovascular Disease and Adiposity
- Pregnancy-related medical research
- Chromatin Remodeling and Cancer
- Ion channel regulation and function
- Colorectal and Anal Carcinomas
- FOXO transcription factor regulation
- Gene Regulatory Network Analysis
- Cancer Mechanisms and Therapy
- Optical Imaging and Spectroscopy Techniques
Niigata University
2016-2024
University of Virginia
2023-2024
Niigata University Medical and Dental Hospital
2020-2021
The fundamental morphology of the endometrial glands is not sufficiently understood by 2D observation because these have complicated winding and branching patterns. To construct a large picture gland structure, we performed tissue-clearing-based 3D imaging human uterine tissue. Our immunohistochemistry layer analyses revealed that form plexus network in stratum basalis expand horizontally along muscular layer, similar to rhizome grass. We then extended our method assess tissue affected...
Proactive recommendations for human papillomavirus (HPV) vaccines in Japan have been suspended 5 years because of safety concerns. While no scientific evidence exists to substantiate these concerns, one reason given not reinstating is the lack reliable vaccine effectiveness (VE) data a Japanese population. This study reports VE bivalent HPV women aged 20-22 years.During cervical screening between 2014 and 2016, had Papanicolaou smears tests performed provided about their sexual history....
It has become evident that somatic mutations in cancer-associated genes accumulate the normal endometrium, but spatiotemporal understanding of evolution and expansion mutant clones is limited. To elucidate timing mechanism clonal we sequence 1311 endometrial glands from 37 women. By collecting different parts show multiple with same occupy substantial areas endometrium. We demonstrate "rhizome structures", which basal run horizontally along muscular layer vertical rise gland, originate...
We explored the frequency of germline and somatic mutations in homologous recombination (HR)-associated genes major histological types ovarian cancer. performed targeted sequencing to assess 16 HR-associated 4 mismatch repair (MMR) among 207 cancer patients (50 high-grade serous carcinomas (HGSC), 99 clear cell (CCC), 39 endometrioid (EC), 13 mucinous (MC), 6 low-grade (LGSC)). Germline or were detected 44% HGSC, 28% CCC, 23% EC, 16% MC, 17% LGSC patients. The profile gene was remarkably...
Objective: In Japan, the possible adverse events upon HPV vaccination was widely reported in media. MHLW announced suspension of aggressively encouraging 2013, and inoculation rate has sharply declined. The aim present study estimation future cervical cancer risk.Methods: latest data on at each age Sakai City were first investigated. experiencing sexual intercourse 12, 13, 14, 15, 16, 17 throughout lifetime is assumed to be 0%, 1%, 2%, 5%, 15%, 25%, 85% respectively. risk regarded...
Abstract Molecular characteristics of carcinoma arising from mature cystic teratoma the ovary (MCT) remain unclear due to its rarity. We analyzed RNA-sequencing data 2322 pan-cancer [1378 squamous cell carcinomas (SCC), 6 adenosquamous (ASC), and 938 adenocarcinomas (AC)] including six MCT (four SCCs, one ASC, AC). Hierarchical clustering principal component analysis showed that gene expression profiles were different between each histological type SCCs (MCT-SCCs) was apparently similar...
In Japan, recommendations for HPV vaccines were suspended in 2013 due to unfounded safety fears. Although vaccine opponents claim modifying sexual behavior can prevent cervical cancer, no comprehensive data exist on and the risk of high-grade disease a Japanese population. This study investigates infection 3968 women aged 20-41 yrs undergoing screening between April 2014 March 2016. Mean age at first intercourse was 18.4 ± 2.8 32% reported ≥ 6 lifetime partners. regression analyses, number...
Japan has no national vaccine registry and approximately 1700 municipalities manage the immunization records independently. In June 2013, proactive recommendations for human papillomavirus (HPV) were suspended after unconfirmed reports of adverse events following in media, despite safety signal having been raised. Furthermore, studies assessing HPV effectiveness published post suspension are predominantly based on self-reported information. Our aim was to examine accuracy vaccination status...
We have expressed our concern about HPV vaccination coverage in Japan remaining below 1%, which will result an increase vaccine-targeted infection rates and cervical cancer morbidity [1Simms KT Hanley SJB Smith MA Keane A Canfell K. Impact of vaccine hesitancy on Japan: a modelling study.The Lancet Public health. 2020; 5 (-e34): e223Summary Full Text PDF PubMed Scopus (81) Google Scholar, 2Ueda Y Yagi Ikeda S Enomoto T Kimura T. Beyond resumption the Japanese Government's recommendation...
Abstract ARID1A loss-of-function mutation accompanied by a loss of protein expression is considered one the most important driver events in endometriosis-associated ovarian cancer. Although our recent genomic study clarified that mutations were detected 13% endometriosis, an association between status and endometriosis remains unclear. We performed immunohistochemical staining for 78 samples 99 clear cell carcinoma samples. revealed not only 70 without but also eight with retained...
Abstract KRAS is the most frequently mutated in ovarian endometriosis. However, it unclear whether mutant allele's mRNA expressed and plays a biological role Here, we performed mutation‐specific RNA situ hybridization to evaluate allele expression of p.G12V, detected mutation endometriosis our previous study, formalin‐fixed paraffin‐embedded tissue (FFPE) samples endometriosis, cancer cell lines, cancers. First, verified that or wild‐type were all 5 lines 9 cases corresponding status. Next,...
In Japan, public funding for HPV vaccination began in 2010 girls aged 13-16 years (birth cohort 1994-1997) and women born 1994 who turned 25 2019. We aimed to verify the long-term effectiveness of bivalent vaccine years. Subjects were 25-26 underwent cervical cancer screening testing Niigata from 2019 2020 1993-1994). Information on status sexual behavior was obtained a questionnaire municipal records. compared infection rates vaccinated unvaccinated groups. Of 429 registrants, 150 (35.0%)...
Abstract BRCA1/2 mutations are robust biomarkers for platinum-based chemotherapy in epithelial ovarian cancers. However, clear cell carcinoma (CCC) less frequent compared with high-grade serous cancer (HGSC). The discovery of that can be applied to CCC is an unmet need chemotherapy. Schlafen 11 (SLFN11) has attracted attention as a novel sensitizer DNA-damaging agents including platinum. In this study, we investigated the utility SLFN11 HGSC and expression was analyzed retrospectively by IHC...
In Japan, the rate of routine cervical cancer screening is quite low, and incidence has recently been increasing. Our objective was to investigate ways effectively influence parental willingness recommend that their 20-year-old daughters undergo screening.We targeted parents whose were living with them. fiscal year 2013, as usual, daughter received a reminder postcard several months after they had free coupon for screening. 2014, information leaflet, well cartoon about show daughters,...
Organized human papillomavirus vaccination (OHPV) in Japan was introduced 2010 for girls aged 12-16 years who were born 1994 or later. The rate of OHPV coverage 70-80%. However, after suspension the government recommendation, dramatically decreased. We aim to investigate change prevalence HPV infection initiation vaccination. recruited females 20-21 attending public cervical cancer screening from 2014 2017 fiscal (April March 2018). Residual Pap test specimens collected testing. compared...
Japanese girls aged 12-16 years are offered free human papillomavirus (HPV) vaccination and cervical cancer screening is conducted with cytology not HPV testing from the age of 20 years. So far, no study has analyzed effect against precancers considering infection status sexual activity. We aimed to analyze vaccine effectiveness (VE) cytological abnormalities, adjusted for This comprised women 20-26 who underwent in Niigata. obtained municipal records a questionnaire along information...
BackgroundAlthough ovarian clear cell carcinoma (CCC) is associated with high incidence of thromboembolism, the clinicopathological and biological significance hypercoagulable status in CCC remains unclear.Materials methodsWe retrospectively analyzed pretreatment D-dimer levels, thromboembolic status, clinical outcome 125 CCCs discovery set 143 two other independent validation sets. Next, we performed RNA sequencing 93 compared coagulation-related gene profiles 2492 pan-cancer data. We...
Advanced-stage gynecologic cancer remains a life-threatening disease. Here, we present protocol for establishment of stable in vitro 3D spheroid cells derived from human uterine endometrial and ovarian tissues. The tumor-derived have stem cell-related characteristics, including tumorigenesis, can be used biological biochemical analyses drug efficacy assays. Because these possess the characteristics original tumors, spheroid-derived xenografts will applications personalized medicine future....