Jonathan Lambourne
A5070681956- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Antifungal resistance and susceptibility
- Mycobacterium research and diagnosis
- Cytomegalovirus and herpesvirus research
- Fungal Infections and Studies
- Immunodeficiency and Autoimmune Disorders
- Autoimmune and Inflammatory Disorders Research
- Viral Infections and Vectors
- Neutropenia and Cancer Infections
- Mosquito-borne diseases and control
- Parvovirus B19 Infection Studies
- COVID-19 and healthcare impacts
- Antimicrobial Resistance in Staphylococcus
- Plant Pathogens and Fungal Diseases
- vaccines and immunoinformatics approaches
- Herpesvirus Infections and Treatments
- COVID-19 epidemiological studies
- Infective Endocarditis Diagnosis and Management
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial Identification and Susceptibility Testing
- Complement system in diseases
- Hepatitis B Virus Studies
- Respiratory viral infections research
- Long-Term Effects of COVID-19
Barts Health NHS Trust
2016-2025
National Health Service
2025
Royal London Hospital
2017-2024
University College London
2013-2023
Hospital for Tropical Diseases
2015-2023
St Bartholomew's Hospital
2020-2022
King's College Hospital NHS Foundation Trust
2017
King's College London
2017
University College Hospital
2013-2015
University College London Hospitals NHS Foundation Trust
2015
Abstract Individuals with potential exposure to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) do not necessarily develop PCR or antibody positivity, suggesting that some individuals may clear subclinical infection before seroconversion. T cells can contribute the rapid clearance of SARS-CoV-2 and other infections 1–3 . Here we hypothesize pre-existing memory cell responses, cross-protective against (refs. 4–11 ), would expand in vivo support viral control, aborting infection....
A boost from infection During clinical trials of severe acute respiratory syndrome coronavirus 2 vaccines, no one who had survived with the virus was tested. year after pandemic declared, vaccination previously infected persons is a reality. Reynolds et al. address knowledge gap in cohort UK health care workers given Pfizer/BioNTech vaccine which half participants experienced natural infections early (see Perspective by Crotty). Genotyping indicated that genetic component underlies...
The Omicron, or Pango lineage B.1.1.529, variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) carries multiple spike mutations with high transmissibility and partial neutralizing antibody (nAb) escape. Vaccinated individuals show protection against disease, often attributed to primed cellular immunity. We investigated T B cell immunity B.1.1.529 in triple BioNTech BNT162b2 messenger RNA-vaccinated health care workers (HCWs) different SARS-CoV-2 infection histories....
Understanding the nature of immunity following mild/asymptomatic infection with SARS-CoV-2 is crucial to controlling pandemic. We analyzed T cell and neutralizing antibody responses in 136 healthcare workers (HCW) 16-18 weeks after United Kingdom lockdown, 76 whom had captured by serial sampling. Neutralizing antibodies (nAb) were present 89% previously infected HCW. tended be lower asymptomatic than those reporting case-definition symptoms COVID-19, while nAb titers maintained irrespective...
BackgroundSARS-CoV-2 serology is used to identify prior infection at individual and population level. Extended longitudinal studies with multi-timepoint sampling evaluate dynamic changes in antibody levels are required the time horizon which these applications of valid, explore longevity protective humoral immunity.MethodsHealthcare workers were recruited a prospective cohort study from first SARS-CoV-2 epidemic peak London, undergoing weekly symptom screen, viral PCR blood over 16–21 weeks....
BackgroundWe hypothesised that host-response biomarkers of viral infections might contribute to early identification individuals infected with SARS-CoV-2, which is critical breaking the chains transmission. We aimed evaluate diagnostic accuracy existing candidate whole-blood transcriptomic signatures for infection predict positivity nasopharyngeal SARS-CoV-2 PCR testing.MethodsWe did a nested case-control study among prospective cohort health-care workers (aged ≥18 years) at St Bartholomew's...
Invasive aspergillosis is a devastating infection with attributable mortality of 40% despite antifungal therapy. In animal models aspergillosis, deficiency mannose-binding lectin (MBL), pattern recognition receptor that activates complement, susceptibility factor. MBL occurs in 20%-30% the population. We hypothesized may be factor for invasive humans.Serum concentrations were measured by enzyme-linked immunosorbent assay 65 patients proven or probable acute and 78 febrile immunocompromised...
The integration of long-read sequencing technology, such as nanopore technology [Oxford Nanopore Technologies (ONT)], into routine diagnostic laboratories has the potential to transform bacterial infection diagnostics and improve patient management. Analysis amplicons from 16S rRNA gene generates a comprehensive view microbial community within clinical samples, significantly enhancing sensitivity capacity analyse mixed populations compared short read approaches. This study evaluates various...
Background Candida auris is an emerging multidrug-resistant fungal pathogen associated with bloodstream, wound and other infections, especially in critically ill patients. C. carriage persistent difficult to eradicate from the hospital environment. Aim We aimed pilot admission screening for intensive care units (ICUs) England estimate prevalence ICU population inform public health guidance. Methods Between May 2017 April 2018, we screened admissions eight adult ICUs hospitals no previous...
Objective The aim of this study was to assess the efficacy, safety and tolerability pegylated interferon ribavirin in HIV/hepatitis C virus (HCV)‐coinfected patients, prescribed for same duration at dosage as that used HCV monoinfection studies. Design It an open‐label, single‐centre, prospective study. Methods Forty‐five patients coinfected with HIV CD4 counts >200 cells/μL were treated interferon‐ α2b 1.5 μg/kg/week 1000–1200 mg/day 24–48 weeks depending on genotype. Safety assessed...
Pleural infections cause major morbidity and mortality, particularly amongst paediatric elderly populations. The aetiology is broad, but pleural culture fails to yield a causative pathogen in approximately 40 % of cases. Alternative identification methods are therefore required. aim the study was investigate from impact on patient care when performing 16S rRNA PCR culture-negative fluid specimens determine whether any individual laboratory parameters were associated with positive result. We...
Determining the protection an individual has to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants of concern (VoCs) is crucial for future immune surveillance, vaccine development, and understanding changing response. We devised informative assay current ELISA-based serology using multiplexed, baited, targeted proteomics direct detection multiple proteins in SARS-CoV-2 anti-spike antibody immunocomplex. Serum from individuals collected after infection or first- second-dose...
We present two cases of cryptococcal meningitis in people subsequently diagnosed with idiopathic CD4+ lymphopenia. Both presented new onset headaches without sinister features and were sent home on multiple occasions from emergency departments. Cryptococcal HIV-negative patients poses major diagnostic management problems; the associated mortality is 9%–27%. suggest performing blood cerebrospinal fluid antigen tests all lymphocytic meningitis.
Abstract Studies of adaptive immunity to SARS-CoV-2 include characterisation lethal, severe and mild cases 1-8 . Understanding how long lasts in people who have had or asymptomatic infection is crucial. Healthcare worker (HCW) cohorts exposed infected by during the early stages pandemic are an invaluable resource study this question 9-14 The UK COVIDsortium a longitudinal, London hospital HCW cohort, followed from time lockdown 9,10 ; weekly PCR, serology symptom diaries allowed capture...
<h3>ABSTRACT</h3> <i>Brucella</i> and <i>Coxiella</i> are similar; both obligate intracellular, zoonotic pathogens with a broad geographic distribution. Infection in animals is usually asymptomatic, but causes fetal loss therefore has significant economic impact. Human infection may be asymptomatic or give rise to either organ-specific multi-system disease. Organism culture challenging for can lack sensitivity <i>Brucella</i>. Therefore, most commonly diagnosed by serology, this negative...
Objective: To describe a case of delayed-type hypersensitivity (DTH) reaction to piperacillin/tazobactam in which painful paresthesiae were predominant feature. Case Summary: A 27-year-old man with history intravenous drug abuse was admitted for treatment pulmonary parenchymal abscess the setting lower-limb deep-venous thrombosis and methicillin-sensitive Staphylococcus aureus bacteremia. He treated 4.5 g 3 times daily; however, after 2 weeks therapy, he developed symptoms (eg, fever,...
Abstract We hypothesised that host-response biomarkers of viral infections may contribute to early identification SARS-CoV-2 infected individuals, critical breaking chains transmission. identified 20 candidate blood transcriptomic signatures infection by systematic review and evaluated their ability detect infection, compared the gold-standard virus PCR tests, among a prospective cohort 400 hospital staff subjected weekly testing when fit attend work. The transcriptional had limited overlap,...
Reports of methicillin-resistant Staphylococcus aureus (MRSA) harboring the mecC gene have increased in UK since first being described. To our diagnostic S. multiplex PCR, a primer set was designed and implemented, then prevalence patient population investigated. Fewer than 1% clinical isolates possessed gene, confirming that mecA remains dominant genetic determinant MRSA East London.
Abstract Background SARS-CoV-2 serology is used to identify prior infection at individual and population level. Extended longitudinal studies with multi-timepoint sampling evaluate dynamic changes in antibody levels are required the time horizon which these applications of valid, explore longevity protective humoral immunity. Methods Health-care workers were recruited a prospective cohort study from first epidemic peak London, undergoing weekly symptom screen, viral PCR blood over 16-21...