A5047027663- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- IL-33, ST2, and ILC Pathways
- Dermatology and Skin Diseases
- Eosinophilic Esophagitis
- Transgenic Plants and Applications
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Contact Dermatitis and Allergies
- Cancer Immunotherapy and Biomarkers
- Viral gastroenteritis research and epidemiology
- Celiac Disease Research and Management
- Drug-Induced Adverse Reactions
- Mast cells and histamine
- Diabetes and associated disorders
- Acute Kidney Injury Research
- Viral Infections and Immunology Research
- Immune cells in cancer
- CAR-T cell therapy research
- Respiratory viral infections research
- Listeria monocytogenes in Food Safety
- Pancreatic function and diabetes
Virginia Mason Medical Center
2014-2025
Benaroya Research Institute
2016-2025
Icahn School of Medicine at Mount Sinai
2023-2025
Stallergenes Greer (France)
2008-2011
A unique T helper cell signature in allergic patients isolates the pathogenic cells and provides a target for disease intervention.
Abstract In this study, we used HLA-DRB1*0101, DRB1*0401, and DRB1*1501 peptide tetramers combined with cytokine surface capture assays to characterize CD4+ T cell responses against the immunodominant epitope (peptide 141–155) from major birch pollen allergen Bet v 1, in both healthy allergic individuals. We could detect 1-specific cells PBMC of 20 patients, but also 9 individuals tested. Analysis at a single-cell level revealed that allergen-specific secrete IFN-γ IL-10 response allergen,...
Abstract The significance of islet Ag-reactive T cells found in peripheral blood type 1 diabetes (T1D) subjects is unclear, partly because similar are also healthy control (HC) subjects. We hypothesized that key disease-associated would show evidence prior Ag exposure, inferred from expanded TCR clonotypes, and essential phenotypic properties their transcriptomes. To test this, we developed single-cell RNA sequencing procedures for identifying clonotypes transcript phenotypes individual...
The PALISADE study, an international, phase 3 trial of peanut oral immunotherapy (POIT) with AR101, resulted in desensitization children and adolescents who were highly allergic to peanut. An improved understanding the immune mechanism induced response food allergen would enable more informed effective therapeutic strategies. Our main purpose was examine immunological changes blood samples from a subset peanut-allergic individuals undergoing AR101.
House dust mite (HDM) allergies are prevalent, yet current treatments like allergen avoidance, pharmacotherapy, and conventional immunotherapy present limitations. The novel LAMP (lysosomal-associated membrane protein)-based DNA vaccine ASP2390 targets major HDM allergens, potentially shifting immune responses toward nonallergic pathways minimizing the risk of atopy, with positive safety efficacy signals in preclinical models. We evaluated safety, tolerability, first-in-human intradermal...
The US Food and Drug Administration (FDA) hosted a workshop on February 22, 2024, to discuss the status of biomarkers in drug development for allergic asthma food allergy. provided forum open discussion among regulators, academicians, NIH staff industry inform stakeholders requirements FDA adopt biomarker as surrogate endpoint clinical trial, various development. was divided into three sessions: 1) EU regulators discussing regulatory perspectives use programs; 2) investigators pediatric...
Surrogate biomarkers of efficacy are needed in support allergen-specific immunotherapy.The aim this study was to relate changes peripheral CD4(+) T cell responses clinical during sublingual immunotherapy (SLIT).Allergen-specific were assessed blood mononuclear cells (PBMCs) from 89 grass pollen-allergic individuals enrolled a double-blind placebo-controlled SLIT conducted an allergen exposure chamber (ClinicalTrials.gov NCT00619827). Surface phenotype, proliferative responses, cytokine...
Checkpoint inhibitors target the inhibitory receptors expressed by tumor-infiltrating T cells in order to reinvigorate an anti-tumor immune response. Therefore, understanding cell composition and phenotype human tumors is crucial. We analyzed flow cytometry lymphocytes (TILs) from two independent cohorts of patients with different cancer types, including RCC, lung colon cancer. In healthy donors, peripheral are usually either CD4+ or CD8+ a small percentage DP (5%, reaching 30-50% some...
A better understanding of allergen-specific CD4(+) T cell responses is needed to help improving immunological therapies. Objective To compare against seasonal (Bet v 1) and perennial (Der p 1, Der 2) allergens.Major histocompatibility complex class II peptide tetramers were engineered monitor responses. After in vitro expansion, tetramer(+) cells tested for surface markers using cytofluorometry. Cytokine gene expression production assessed quantitative PCR cytokine capture assays,...
Conceptually, allergic responses may involve cross-reactivity by antibodies or T-cells. While IgE among grass-pollen allergens has been observed, at the allergen-specific T-cell level less documented. Identification of patterns improve our understanding, allowing optimization better immunotherapy strategies.We use Phleum pratense as model for studying CD4(+) T cell DR04:01 restricted Pooideae epitopes.After in vitro culture blood mono-nucleated cells from grass-pollen-allergic subjects with...