We previously showed that high expression levels of SOX9 correlate with hepatocellular carcinoma (HCC) progression. However, the exact role plays in HCC remains unclear. In this study, we firstly confirmed miRNA‐101 directly targets HCC. Ectopic miR‐101 significantly inhibited cell proliferation and tumorigenicity by targeting SOX9. Moreover, down‐regulation clinical tissues correlates tumor aggressiveness poor prognosis. Therefore, may suppress progression down‐regulating MiR‐101 be a...

Focal bone destruction within inflamed joints is the most specific hallmark of rheumatoid arthritis (RA). Our previous study indicated that therapeutic efficiency triptolide in RA may be due partially to its chondroprotective and anti-inflammatory effects. However, roles are still unclear. In this study, our data firstly showed effects on severity progression collagen-induced (CIA) mice. Then, by micro-CT quantification, treatment significantly increased mineral density, volume fraction,...

Steroid-induced osteonecrosis of the femoral head (steroid-induced ONFH) presents great challenges due to various effects steroids on multi-system pathways involved into osteoblast differentiation, and osteoclast apoptosis, lipid metabolism, calcium metabolism coagulation. As one most frequently used herbs in Traditional Chinese Medicine formulas that are prescribed for regulation bone mineral therapeutic Achyranthes bidentata steroid-induced ONFH remain unclear. Thus, aim current study was...

Steroid-induced osteonecrosis of the femoral head (steroid-induced ONFH) is characterized by increase intraosseous pressure because lipid metabolism disturbance such as elevation adipogenesis and fat cell hypertrophy in bone marrow, subsequently leading to disturbances coagulation-fibrinolysis system finally resulting ischemia. Pravastatin has been demonstrated be useful preventing steroid-induced ONFH animal models. However, its exact mechanisms acting on this disease have not fully...

This study develops a readily implementable surface modification technique for Al alloys by combining electroplating alloy films with depositing stearic acid to make durable superhydrophobic coating. Selecting as anode innovatively, the introduction of element improves adherence and roughness coating through principle identical reactive (RE) effect. can be preferentially combined oxygen reduce partial pressure oxygen, inhibiting outward diffusion oxidation electroplated components, reducing...

Abstract Background Radiation-induced skin injury (RISI) commonly manifests in cancer patients undergoing radiotherapy (RT). However, a universally accepted standard for treating radiation has not yet been established. Our objective was to provide detailed molecular overview of pre- and post-radiation therapy, aiming enhance our understanding the subclusters mechanisms contributing radiodermatitis. Methods C57BL/6 mice were subjected single fraction (20 Gy) RT targeting right dorsal skin. We...

Several complex molecular events are involved in tumorigenesis of hepatocellular carcinoma (HCC). The interactions these molecules may constitute the HCC imbalanced network. Gansui Banxia Tang (GSBXT), as a classic Chinese herbal formula, is popular complementary and alternative medicine modality for treating HCC. In order to investigate therapeutic effects pharmacological mechanisms GSBXT on reversing network, we current study developed comprehensive systems approach integrating...

Sanmiao formula (SM) is a basic prescription for the treatment of gouty and rheumatoid arthritis that has been used in China over long period history. However, there no evidence associating SM with osteoarthritis (OA). In this study, characterization anti-OA effect was conducted using an vivo rat model induced by anterior cruciate ligament transection medial meniscus resection (ACLT plus MMx), together vitro studies chondrocytes further molecular characterization. Rats subjected to ACLT MMx...

Abstract Panax ginseng (GS) and Veratrum nigrum (VN) are representative of incompatible pairs in “eighteen antagonistic medicaments” that have been recorded the Chinese medicinal literature for over 2,000 years. However, evidence linking interference effects with combination use is scare. Based on estrogen-like effect GS described our previous studies, we undertake a characterization interaction estrogenic activity VN using vivo models immature ovariectomized (OVX) mice vitro studies MCF-7...

Hypoxia and sulfide are inducing potential damage to aquatic organisms. However, the effects of hypoxia on their immune systems molecular mechanisms not fully understood. In present study, clam Tegillarca granosa was exposed alone or in combination with (0.1, 0.5 mM) investigate physiological transcriptomic responses haemolymph. The IBR analysis revealed that moderate stimulated via increasing total hemocyte counts, phagocytic activity, antibacterial antioxidant activity. many critical...

A Chinese herbal preparation, SiMiaoFang (SMF), has been used clinically for treating arthralgia by virtue of its anti-inflammatory and pain-relieving activities. However, no evidence base links SMF to anti-osteoarthritis (OA), particularly link inhibiting cartilage matrix degradation. In this study, we undertook a characterization anti-OA activity using an in vivo rat model induced anterior cruciate ligament transection medial meniscus resection (ACLT+MMx) together with vitro studies...

To evaluate the estrogenic efficacy of Renshen (Radix Ginseng) (GS) on reproductive target tissues in immature mice. One hundred and ten female Kunming (KM) mice, 21-day-old, were randomly assigned to eleven groups, 10 for each; one served as control group treated with 0.154 mg/kg estradiol valerate (EV, n = 10), rest respectively GS intragastrically at a daily dose 0.5, 1.0, 1.5, 3.0, 6.0, 12.0, 18.0, 24.0 30.0 g/kg (n per group) 7 days. The estrous cycle, uterine weight, hormone levels...

Traditional Chinese medicines (TCM) contain multi-interactive compounds that have been used for treatment of peri-menopausal syndrome and become a new phytoestrogens resource. The QiBaoMeiRan formula (QBMR), including Polygoni Multiflori Radix, Angelicae Sinensis Achyranthis Bidentatae Semen Cuscutae, Fructus Lycii, Poria, Psoraleae, has clinically treating osteoporosis in post-menopausal women by virtue its kidney-invigorating function. However, no evidence base links QBMR to estrogen...

Chuanwu (CW), the mother root of Aconitum carmichaelii Debx., is a traditional Chinese medicine (TCM) for treating traumatic injuries, rheumatoid arthritis, and tumors. CW coadministered with banxia (BX), Pinellia ternata, also widely prescribed in clinical practice. However, mechanism this combination yet deciphered. Current study aimed to investigate effects CW, including raw chuanwu (RCW) processed (PCW) alone, as well BX on CYP3A activity. Buspirone (BP) testosterone (Tes) were used...

Wu-tou decoction (WTD) is a classic traditional Chinese medicine formula and has been used effectively to treat joint diseases clinically. Previous reports indicated that WTD possesses anti-inflammatory activity; however, its actions on pain have not clarified. Here, we investigated the antinociceptive activity of in CFA-induced mice, possible mechanism action associated with transient receptor potential (TRP) ion channels was also explored. Our results showed 1.58, 3.15, 6.30 g/kg...

To investigate the effects of Huogu I formula on regulation lipid metabolism in steroid-induced osteonecrosis femoral head (SONFH) rats and verify our hypothesis that regulates by down-regulating peroxisome proliferator-activated receptor gamma (PPARgamma) expression activating Wnt signaling pathways.Eighty-five were divided into four groups: control, model, 15 g/kg 30 g/kg. Six weeks later, animals anaesthetized, femora was dissected for histopathological examination osteonecrotic changes...

Abstract The Chinese herbal preparation QiBaoMeiRan formula (QBMR) displayed estrogenic effects in ovariectomized rats after long-term administration a previous study. uterus and vagina are negatively influenced by estrogens hormone therapy. While QBMR is known to be phytoestrogen, its safety on reproductive tissues short-term mechanism via estrogen receptor (ER) pathway haven’t been studied. Here, we characterized using immature mice together with vitro studies for further molecular...

Salvia miltiorrhiza bunge(SM) is a popular herb for alleviating menopausal symptoms, although the scientific evidence of applying SM to estrogen replacement therapy limited. In this study, we characterized estrogenic activity using in vivo models immature and ovariectomized (OVX) mice performed vitro studies focusing on receptor (ER) pathway further molecular characterizations. treatments demonstrated significant by promoting development uterus vagina mice, restoring estrus cycle reversing...

Processed Chuanwu (PCW), the mother root of Aconitum carmichaelii Debeauxv, has been widely used as a classic Traditional Chinese Medicine for pain relieve over two millennia clinically. However, its action on chronic inflammatory not clarified. Here, we investigated antinociceptive effect PCW in complete freund's adjuvant (CFA)-induced mice and possible mechanisms associated with opioid system TRPV1 ion channel.Male ICR were intraplantarly injected CFA. (0.34, 0.68 1.35 g/kg) was orally...