A5000525310- Optical Coherence Tomography Applications
- Photoacoustic and Ultrasonic Imaging
- Advanced Fluorescence Microscopy Techniques
- Coronary Interventions and Diagnostics
- Cardiac Imaging and Diagnostics
- Retinal and Macular Surgery
- Cerebrovascular and Carotid Artery Diseases
- Cardiac Fibrosis and Remodeling
- ECG Monitoring and Analysis
- Atherosclerosis and Cardiovascular Diseases
- Cardiac electrophysiology and arrhythmias
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Angiogenesis and VEGF in Cancer
- Optical Imaging and Spectroscopy Techniques
- Sepsis Diagnosis and Treatment
- Peripheral Artery Disease Management
- Aortic Thrombus and Embolism
- Advanced Fiber Laser Technologies
- Hemodynamic Monitoring and Therapy
- Inflammatory Biomarkers in Disease Prognosis
- Cardiac Arrest and Resuscitation
- Atrial Fibrillation Management and Outcomes
- Cardiac, Anesthesia and Surgical Outcomes
- Artificial Intelligence in Healthcare and Education
- Nitric Oxide and Endothelin Effects
Massachusetts General Hospital
2016-2025
Center for Systems Biology
2014-2025
Harvard University
2016-2025
Harvard–MIT Division of Health Sciences and Technology
2005-2024
Universidad Politécnica de Madrid
2020
University of California, San Diego
2018
Institute of Cardiology
2017
Harbin Medical University
2014-2017
Second Affiliated Hospital of Harbin Medical University
2014-2017
Massachusetts Institute of Technology
2006-2016
<h3>Importance</h3> Coronavirus disease 2019 (COVID-19) is an acute respiratory illness with a high rate of hospitalization and mortality. Biomarkers are urgently needed for patient risk stratification. Red blood cell distribution width (RDW), component complete counts that reflects cellular volume variation, has been shown to be associated elevated morbidity mortality in wide range diseases. <h3>Objective</h3> To investigate whether association between RDW at hospital admission during...
Abstract Tissue macrophage numbers vary during health versus disease. Abundant inflammatory macrophages destruct tissues, leading to atherosclerosis, myocardial infarction and heart failure. Emerging therapeutic options create interest in monitoring patients. Here we describe positron emission tomography (PET) imaging with 18 F-Macroflor, a modified polyglucose nanoparticle high avidity for macrophages. Due its small size, Macroflor is excreted renally, prerequisite the isotope flourine-18....
Sterile tissue injury is thought to locally activate innate immune responses via damage-associated molecular patterns (DAMPs). Whether pathways are remotely activated remains relatively unexplored. Here, by analyzing ~145,000 single-cell transcriptomes at steady state and after myocardial infarction (MI) in mice humans, we show that the type I interferon (IFN) response, characterized expression of IFN-stimulated genes (ISGs), begins far from site injury, neutrophil monocyte progenitors...
Background Neutrophils are thought to be short-lived first responders tissue injuries such as myocardial infarction (MI), but little is known about their diversification or dynamics. Methods and Results We permanently ligated the left anterior descending coronary arteries of mice performed single-cell RNA sequencing analysis >28 000 neutrophil transcriptomes isolated from heart, peripheral blood, bone marrow on days 1 4 after MI at steady-state. Unsupervised clustering cardiac neutrophils...
Abstract Sudden cardiac death, arising from abnormal electrical conduction, occurs frequently in patients with coronary heart disease. Myocardial ischemia simultaneously induces arrhythmia and massive myocardial leukocyte changes. In this study, we optimized a mouse model which hypokalemia combined infarction triggered spontaneous ventricular tachycardia ambulatory mice, showed that major subsets have opposing effects on conduction. Neutrophils increased via lipocalin-2 whereas neutrophilia...
Inflammation is the physiologic reaction to cellular and tissue damage caused by trauma, ischemia, infection, other pathologic conditions. Elevation of white blood cell count (WBC) altered levels acute phase reactants are cardinal signs inflammation, but dynamics these changes their resolution not well established. Here we studied inflammatory recovery from infection tracking longitudinal clinical laboratory measurements in hospitalized patients. We identified a universal trajectory defined...
Optical coherence microscopy (OCM) is demonstrated with a high-speed, broadband, reflective-grating phase modulator and femtosecond Ti:Al2O3 laser. The novel system design permits high-resolution OCM imaging in new operating regime which short gate used to relax the requirement for high-numerical-aperture confocal axial sectioning. In vivo cellular Xenopus laevis tadpole human skin 3-μm 30-μm gate. ability achieve lower numerical aperture should facilitate development of miniaturized probes...
Ultrahigh resolution two and three-dimensional optical coherence tomography (OCT) imaging was performed using a miniaturized, two-axis scanning catheter based upon microelectromechanical systems (MEMS) mirror technology. The incorporated custom-designed fabricated, 1-mm diameter MEMS driven by angular vertical comb (AVC) actuators on both an inner axis outer, orthogonal gimbal axis. Using differential drive scheme, linearized position response over +/- 6 degrees mechanical angle achieved....
Abstract Three-dimensional (3D) tissue imaging methods are expected to improve surgical management of cancer. In this study, we examined the feasibility two 3D technologies, optical coherence tomography (OCT) and microscopy (OCM), view human breast specimens based on intrinsic contrast. Specifically, imaged 44 ex vivo including 34 benign 10 malignant lesions with an integrated OCT OCM system developed in our laboratory. The enabled 4-μm axial resolution (OCT OCM) 14-μm 2-μm (OCM) transverse...
Significance Despite advances in cardiac imaging technologies such as MRI, computed tomography, and ultrasound it has not been possible to image function animal models at the cellular level owing insufficient resolution excess motion. As a result, our understanding of biology is derived from either whole-organ techniques or isolated vitro heart preparations model systems. We demonstrate technique for intravital optical microscopy that compensates motion contracting allows measurement...
Plaque rupture may be the local expression of a widespread coronary instability. This study aimed to investigate: (1) prevalence and characteristics nonculprit plaque rupture; (2) pancoronary atherosclerotic phenotype in patients with without (3) predictors multiple ruptures.Six hundred seventy-five plaques from 261 (34 acute myocardial infarction, 73 unstable angina pectoris, 154 stable pectoris) were analyzed by 3-vessel optical coherence tomography. Nonculprit ruptures identified 51...
Supervised machine learning applications in health care are often limited due to a scarcity of labeled training data. To mitigate the effect small sample size, we introduce pre-training approach, P atient C ontrastive L earning R epresentations (PCLR), which creates latent representations electrocardiograms (ECGs) from large number unlabeled examples using contrastive learning. The resulting expressive, performant, and practical across wide spectrum clinical tasks. We develop PCLR system...
A distally actuated, rotational-scanning micromotor endoscope catheter probe is demonstrated for ultrahigh-resolution in vivo endoscopic optical coherence tomography (OCT) imaging. The permits focus adjustment visualization of tissue morphology at varying depths with improved transverse resolution compared standard OCT imaging probes. distal actuation avoids nonuniform scanning motion artifacts that are present other designs and can permit a wider range speeds. Ultrahigh-resolution rabbit...
Ultrahigh resolution, real time OCT imaging is demonstrated using a compact femtosecond Nd:Glass laser that spectrally broadened in high numerical aperture single mode fiber. A reflective grating phase delay scanner enables broad bandwidth, high-speed group scanning. We demonstrate vivo, ultrahigh at 1 microm center wavelength with <5 axial resolution free space (<4 tissue). The light source robust, portable, and well suited for vivo studies.
Ultrahigh resolution optical coherence tomography (OCT) is demonstrated at 800 nm and 1300 using continuum generation in a single photonic crystal fiber with parabolic dispersion profile two closely spaced zero wavelengths. Both wavelengths are generated simultaneously by pumping the ~78 mW average power 1064 52 MHz, 85 fs pulse train from compact Nd:Glass oscillator. Continuum processes result double peak spectrum > 110 30 150 48 nm. OCT imaging < 5 mum tissue 3 demonstrated. Numerical...
Optical coherence tomography (OCT) is an emerging medical imaging technology that can generate high resolution, cross-sectional images of tissue in situ and real time. Although endoscopic OCT has been used successfully to identify certain pathologies the gastrointestinal tract, resolution current systems limited 10-15 microm for vivo studies. In this study, rabbit tract demonstrated at a three-fold higher (< 5 microm), using broadband Cr(4+):Forsterite laser as optical light source. Images...
Institutional review board approval at the participating institutions was obtained. Informed consent waived for this HIPAA-compliant study. The study purpose to establish correspondence of optical coherence tomographic (OCT) image findings with histopathologic understand which features characteristic breast lesions can be visualized OCT. Imaging performed in 119 specimens from 35 women aged 29-81 years 3.5-microm axial resolution and 6-microm transverse 1.1-microm wavelength on freshly...