A5006181270- Ion channel regulation and function
- Cervical and Thoracic Myelopathy
- Cardiac Valve Diseases and Treatments
- Nitric Oxide and Endothelin Effects
- Aortic Disease and Treatment Approaches
- Spine and Intervertebral Disc Pathology
- Heterotopic Ossification and Related Conditions
- Cardiac electrophysiology and arrhythmias
- Cardiac Ischemia and Reperfusion
- Eicosanoids and Hypertension Pharmacology
- Cardiomyopathy and Myosin Studies
- Calcium signaling and nucleotide metabolism
- Natural Compound Pharmacology Studies
- Hormonal Regulation and Hypertension
- Insect and Pesticide Research
- Venomous Animal Envenomation and Studies
- Adipose Tissue and Metabolism
- Adenosine and Purinergic Signaling
- Immune Response and Inflammation
- Infective Endocarditis Diagnosis and Management
- Mesenchymal stem cell research
- Biochemical effects in animals
- Neuroscience and Neuropharmacology Research
- Cardiac tumors and thrombi
- Endometriosis Research and Treatment
Hirosaki University
2015-2024
Hashimoto Municipal Hospital
2017-2021
Tohoku University
1994-2013
Akita University
2012
Matsuyama Red Cross Hospital
1987-2011
Shizuoka University of Welfare
2001-2005
Health First
2003
Tohoku Medical and Pharmaceutical University
1994-2002
Tokyo Metropolitan University
2002
Kyorin University
2000
Journal Article Regulation of the Cardiac Ryanodine Receptor by Protein Kinase-Dependent Phosphorylation Get access Toshiyuki Takasago, Takasago Department Molecular Physiology, National Cardiovascular Center Research InstituteSuita, Osaka 565 Search for other works this author on: Oxford Academic PubMed Google Scholar Toshiaki Imagawa, Imagawa Ken-ichi Furukawa, Furukawa Tarou Ogurusu, Ogurusu Munekazu Shigekawa The Biochemistry, Volume 109, Issue 1, January 1991, Pages 163–170,...
Calcific aortic valve stenosis (CAS) is the most frequent heart disease in elderly, accompanied by calcification. Tumor necrosis factor-α (TNF-α), a pleiotropic cytokine secreted mainly from macrophages, has been detected human calcified valves. However, role of TNF-α calcification remains unclear. To clarify whether accelerates valves, we investigated effect on interstitial cells (HAVICs) obtained patients with CAS (CAS group) and regurgitation or dissection having noncalcified (control...
Triadin, a calsequestrin-anchoring transmembrane protein of the sarcoplasmic reticulum (SR), was successfully purified from heavy fraction SR (HSR) rabbit skeletal muscle with an anti-triadin immunoaffinity column. Since depletion triadin solubilized HSR column increased [3H]ryanodine binding activity, we tested possibility for negative regulator ryanodine receptor/Ca2+ release channel (RyR). Purified not only inhibited to but also reduced openings RyR incorporated into planar lipid...
Ossification of the posterior longitudinal ligament spine (OPLL) is leading cause myelopathy in Japan and diagnosed by ectopic bone formation paravertebral ligament. OPLL a systemic high mass disease with strong genetic background. To detect genes relevant to pathogenesis OPLL, we performed cDNA microarray analysis systematic gene expression profiles during osteoblastic differentiation cells from patients (OPLL cells), disorder called ossification yellow (OYL), non-OPLL controls together...
Plasma membrane (Ca2+-Mg2+) purified from bovine aortic microsomes by calmodulin affinity chromatography was incorporated into soybean phospholipid liposomes. In the reconstituted proteoliposomes, a protein corresponding to ATPase phosphorylated [γ-32] in presence of cGMP and dependent kinase. Both for Ca2+ maximum uptake activity proteoliposomes were increased cGMP-dependent phosphorylation, there good parallelism between rate extent phosphorylation. These results strongly suggest that...
Platelet-rich plasma (PRP) and hyaluronic acid (HA) injection are both therapeutic options for osteoarthritis chronic tendinopathy. Although several comparative studies on the two have been published, effects of mixing PRP HA not fully understood. The purpose this study is to investigate influence platelets in by measuring releasing growth factors. was produced from nine healthy adult volunteers (mean age, 32.8 ± 2.9 years; range, 29–37) with a commercial separation system. weight-average...
Calcified aortic valve stenosis (CAS) is a common heart disease in elderly people, and mostly accompanied by ectopic calcification. We recently demonstrated that tumor necrosis factor-α (TNF-α) induces calcification of human interstitial cells (HAVICs) obtained from CAS patients. In this study, we investigated the role matrix Gla protein (MGP), known inhibitor antagonizes bone morphogenetic 2 (BMP2) TNF-α-induced HAVICs. HAVICs isolated valves were cultured, was significantly induced with 30...
Orexins (OXs) regulate wakefulness, and a lack of OX Type-I receptors cause narcolepsy. selectively increases norepinephrine (NE) release from rat cerebral cortical slices, brain noradrenergic neurons are involved in the sleep-wakefulness cycle. Ketamine NE cortex. We hypothesized that would affect ketamine anesthesia's interactions with neuronal activity.We used Sprague Dawley rats. studied 1) vivo effects orexin A (OXA) SB-334867-A (Orexin-1 receptor antagonist) on ketamine-induced...
Four corticotropin-releasing factor (CRF)-related peptides have been found in mammals and are known as CRF, urocortin, urocortin II, III (also stresscopin). The three urocortins considerably higher affinities for CRF receptor type 2 (CRF R2) than II highly selective R2. In the present study, authors examined hypothesis that or III, addition to produces vasodilation a candidate natural ligands of R2beta rat thoracic aorta. Involvement protein kinases on urocortin-induced was also explored....
Goniodomin A, one of the polyether macrolides, isolated from dinoflagellate Goniodoma pseudogoniaulax stimulated skeletal actomyosin ATPase activity 2-3 times, with a peak at 3 × 10(-8) to 10(-7) M. The effect goniodomin A was dependent on concentration actin, but not myosin. increased by pretreatment actin (but myosin) A. induced sustained and concentration-dependent increase in fluorescence intensity (excitation wavelength, 277 nm; emission 329 nm) actin. maximum response obtained...
Sarcopenia is characterized by the age-related loss of muscle mass and strength. One mechanisms sarcopenia in function number satellite cells. Royal jelly (RJ) a health food used worldwide. To obtain better digestion absorption than RJ, protease-treated RJ (pRJ) has been developed. pRJ have suggested to potential pharmacological benefits such as prolonging life span reducing fatigue. Because these effects may improve functions cells, we examined or treatment on skeletal muscles an animal...
Ossification of the posterior longitudinal ligament spine (OPLL) is characterized by ectopic bone formation in spinal ligaments, and mechanical stress has been suggested to play an important role progression OPLL. To identify genes that participate OPLL, differential display reverse transcription-polymerase chain reaction (RT-PCR) method was used. A 283-base pair cDNA fragment corresponding prostaglandin I<sub>2</sub> (PGI<sub>2</sub>) synthase highly expressed OPLL cells compared with...
To better understand the molecular pathogenesis of OPLL (ossification posterior longitudinal ligament) spine, an ectopic bone formation disease, we performed cDNA microarray analysis on cultured ligament cells from patients. We found that TSG-6 (tumour necrosis factor α-stimulated gene-6) is down-regulated during osteoblastic differentiation. Adenovirus vector-mediated overexpression inhibited differentiation human mesenchymal stem induced by BMP (bone morphogenetic protein)-2 or OS...
Mesenchymal stem cells (MSCs) isolated from spinal ligaments with ectopic ossification have a propensity toward the osteogenic lineage. To explore epigenetic control of features MSCs, we treated MSCs obtained yellow ligament (OYL) patients and non-OYL DNA methyltransferase inhibitor, 5-aza-2′-deoxycytidine (5AdC). We compared groups (untreated 5AdC) OYL by genome-wide microarray analysis. Next, used methylated immunoprecipitation combined quantitative real-time PCR to assess gene...
For the elucidation of diversity secondary metabolites Dictyostelium cellular slime molds, we investigate constituent three species molds. From methanol extract their fruit bodies, obtained novel compounds, dictyopyrone A (1) and B (2) from D. discoideum rhizoposium C (3) longosporum. They possess a unique α-pyrone moiety with side chain at C-3 position. Their relative structures were elucidated by spectral means, absolute configuration was confirmed asymmetric synthesis 1. Since these...