A5101867711- interferon and immune responses
- Immune Response and Inflammation
- NF-κB Signaling Pathways
- Hepatitis B Virus Studies
- Inflammasome and immune disorders
- Yersinia bacterium, plague, ectoparasites research
- Pharmacological Effects of Natural Compounds
- DNA Repair Mechanisms
- Cytokine Signaling Pathways and Interactions
- Animal Virus Infections Studies
- Bone Tissue Engineering Materials
- Circular RNAs in diseases
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- AI in cancer detection
- Immunotherapy and Immune Responses
- Cancer-related Molecular Pathways
- Cardiac Structural Anomalies and Repair
- Microtubule and mitosis dynamics
- Carcinogens and Genotoxicity Assessment
- Colorectal Cancer Screening and Detection
- 3D Printing in Biomedical Research
- Multi-Agent Systems and Negotiation
- Electrospun Nanofibers in Biomedical Applications
- Protein Kinase Regulation and GTPase Signaling
Worcester Polytechnic Institute
2023
Shaoxing University
2023
Cooper Union
2021
Beijing Biocytogen (China)
2019
Anhui University
2014-2015
Czech Academy of Sciences, Institute of Biotechnology
2010
Jilin University
2009-2010
Guangxi Medical University
2009
National Natural Science Foundation of China
2008
Duke Medical Center
2008
Previous studies have shown that both hepatitis A virus and C inhibit innate immunity by cleaving the mitochondrial antiviral signaling (MAVS) protein, an essential component of virus-activated pathway activates NF-kappaB IFN regulatory factor-3 to induce production type I IFN. For human B (HBV), s-Ag, e-Ag, or HBV virions been suppress TLR-induced activity with reduced IFN-beta subsequent induction IFN-stimulated genes. However, HBV-mediated suppression RIG-I-MDA5 is unknown. In this study,...
Stringent control of inflammasome signaling pathway is important for maintaining immunological balance, yet the molecular mechanisms responsible its tight regulation are still poorly understood. In this study, we found that dependent on mitochondrial antiviral protein (MAVS) was required optimal activation apoptosis-associated specklike (ASC)-dependent inflammasome. particular, TNFR-associated factor 3 to be a direct E3 ligase ASC. Ubiquitination ASC at Lys(174) critical speck formation and...
Abstract Innate immunity to viruses involves receptors such as Retinoic Acid Induced Gene-1 (RIG-I), which senses viral RNA and triggers a signaling pathway involving the outer mitochondrial membrane protein antiviral (MAVS). Recent work has identified that NLRX1, member of another class innate immune receptors, sequesters MAVS away from RIG-I thereby prevents immunity. In this study, we demonstrate proteasome PSMA7 (α4) subunit associates with in vivo vitro. Expression results potent...
ABSTRACT Retinoic acid-inducible gene I (RIG-I) is an intracellular RNA virus sensor that induces type interferon-mediated host-protective innate immunity against viral infection. Although cylindromatosis (CYLD) has been shown to negatively regulate antiviral response by removing K-63-linked polyubiquitin from RIG-I, the regulation of its expression and underlying regulatory mechanisms are still incompletely understood. Here we show RIG-I activity regulated inhibition CYLD mediated microRNA...
The mitochondrial antiviral signaling protein MAVS (IPS-1, VISA, or Cardif) plays an important role in the host defense against viral infection by inducing type I interferon. Recent reports have shown that is also critical for virus-induced apoptosis. However, mechanism of MAVS-mediated apoptosis induction remains unclear. Here, we show binds to voltage-dependent anion channel 1 (VDAC1) and induces caspase-3 activation, which independent its innate immunity. modulates VDAC1 stability...
Significance We investigated the possible role of TANK-binding kinase 1 (TBK1) protein in tamoxifen resistance and found that phosphorylation by TBK1 at Ser-305 site stabilized estrogen receptor α (ERα) modulated its transcriptional activity. Ectopic expression rendered breast cancer cells resistant to tamoxifen. inhibition sensitized tamoxifen-induced cell death. The was increased subjects with positively correlated ERα, ERα Ser-305, cyclin D1 expression. Subjects tumors highly expressed...
Abstract IFN regulatory factors play a pivotal role in many cellular processes, including inflammatory and immune responses. Their activation is tightly regulated by TANK-binding kinase 1 (TBK1). In response to microbial components, TBK1 activates factor 3 (IRF3) cytokine expression. this article, we show that novel target of the IpaH4.5 protein, Shigella type III effector possessing E3 ubiquitin ligase activity. Remarkably, interacts with promotes its K48-linked polyubiquitylation....
Yersinia pestis uses type III effector proteins to target eukaryotic signaling systems. The outer protein (Yop) M from the Y. strain is a critical virulence determinant; however, its role in pathogenesis just beginning emerge. Here we first identify YopM as structural mimic of bacterial IpaH E3 ligase family vitro, and establish that conserved CLD motif N-terminal responsible for function. Furthermore, show NLRP3 novel protein. Specially, associates with NLRP3, mediates activating K63-linked...
During the past decades, immunotherapy, especially antibody-mediated immune checkpoint blockade (ICB) has shown durable tumor inhibition and changed paradigm of cancer treatment. However, a growing body evidence suggests that ICB treatment induces severe immune-related adverse events (irAEs), side effect even leads to discontinuation lifesaving Here, we found colitis in melanoma patients promotes infiltration CD8 + effector T cells into colitic lesions. Further transcriptomic dissection...
MINT‐7297498, MINT‐7297511, MINT‐7297557, MINT‐7297574: MAVS (uniprotkb:Q7Z434) physically interacts (MI:0915) with c‐Abl (uniprotkb:P00519) by anti tag coimmunoprecipitation (MI:0007)MINT‐7297542: bait (MI:0006)MINT‐7297526: far western blotting (MI:0047)
AIM:To investigate whether hepatitis B virus (HBV) infection activates DNA damage response and repair cofactors inhibit HBV replication.METHODS: Human hepatocyte cell line HL7702 was studied.Immunoblotting performed to test the expression of ataxia telangiectasia-mutated (ATM)-Rad3-related protein (ATR), p21 level phosphorylation Chk1, p53, H2AX, ATM in HBVinfected or non-infected-cells.Special short RNAi oligos transfected induce transient ATR knockdown HL7702.ATR-ATM chemical inhibitors...
Background Innate immunity to viruses involves receptors such as RIG-I, which senses viral RNA and triggers an IFN-β signaling pathway involving the outer mitochondrial membrane protein MAVS. However, functional status of MAVS phosphorylation remains elusive. Methodology/Principal Findings Here we demonstrate for first time that undergoes extensive tyrosine upon infection, indicating might play important role in function. A tyrosine-scanning mutational analysis revealed tyrosine-9 (Y9) is a...
There has been a growing interest in enhancing rule-based agent-based models (ABMs) for social media platforms (\emph{i.e.}, X, Reddit) with more realistic large language model (LLM) agents, thereby allowing nuanced study of complex systems. As result, several LLM-based ABMs have proposed the past year. While they hold promise, each simulator is specifically designed to particular scenario, making it time-consuming and resource-intensive explore other phenomena using same ABM. Additionally,...
Abstract Passive mechanical reinforcement of the infarcted heart has been shown to counteract infarct expansion and left ventricular (LV) functional degradation. However, traditional patch plasty ischemic region also restricts diastolic filling, reducing cardiac output. These negative side-effects can be minimized with strategic modification standard patch, suggesting further improvements could possible through a broader exploration materials. This study examines potential advantages graft...
The phospholipase C (PLC) is a family of kinases that hydrolyze phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] to generate two second messengers, inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG), which stimulate distinct downstream signaling.Recently, it has been reported PLC signaling activated by multiple viruses for efficient replication the virus-induced inflammatory response.In this study, we demonstrated PLC-specific inhibitor U73122 strongly suppressed porcine...