Bionanotechnology is the combination of biotechnology and nanotechnology for development biosynthetic environmentally friendly nanomaterial synthesis technology. The presented research work adopted a reliable sustainable approach to manufacturing silver nanoparticles from Brachychiton populneus (BP-AgNPs) leaf extract in aqueous medium. populneus-derived were characterized by UV–Vis spectroscopy, Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy...

In this study, the antibacterial and antifungal properties of silver nanoparticles synthesized with aqueous plant extract Acer oblongifolium leaves were defined using a simplistic, environmentally friendly, reliable, cost-effective method. The oblongifolium, which served as capping reducing agent, was used to biosynthesize nanoparticles. UV visible spectroscopy, X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), scanning electron microscopy analyze biosynthesized...

The important role of Lactiplantibacillus plantarum strains in improving the human mucosal and systemic immunity, preventing non-steroidal anti-provocative drug-induced reduction T-regulatory cells, as probiotic starter cultures food processing has motivated in-depth molecular genomic research these strains. current study, building on this concept, reveals importance 13-3 a potential bacteriocin-producing strain that helps condition digestive system thus enhances immunity living beings via...

Mitochondrial aminoacyl-tRNA synthetases (aaRSs) are essential enzymes in protein synthesis since they charge tRNAs with their cognate amino acids. Mutations the genes encoding mitochondrial aaRSs have been associated a wide spectrum of human diseases. Here we report identification pathogenic mutations (a partial genomic deletion and highly conserved p. Asp325Tyr missense variant) FARS2, gene phenylalanyl-tRNA synthetase, patient early-onset epilepsy isolated complex IV deficiency muscle....

Abstract Disorders of the mitochondrial genome cause a wide spectrum disease, these present mainly as neurological and/or muscle related pathologies. Due to intractability human there are currently no effective treatments for disorders. The majority pathogenic mutations lie in genes encoding tRNA s. Consequently, biochemical deficiency is due protein synthesis defects, which manifest aberrant cellular respiration and ATP synthesis. It has previously been reported that overexpression...

Natural products, medicinal plants, and phytoconstituents serve as important sources accelerators for anticancer drug discovery, especially when they are combined with virtual screening molecular simulations against targets. Proto-oncogene serine/threonine-protein kinase Pim1 (PIM1) is involved in cell survival proliferation, great relevance oncogenesis. PIM1 plays a major role the progression of various common complex human cancers, including prostate cancer, acute myeloid leukemia, other...

Providencia heimbachae, a Gram -ve, rod-shaped, and opportunistic bacteria isolated from the urine, feces, skin of humans engage in wide range infectious diseases such as urinary tract infection (UTI), gastroenteritis, bacteremia. This bacterium belongs to Enterobacteriaceae family can resist antibiotics known multidrug-resistant (MDR), be life-threatening humans. After retrieving whole proteomic sequence P. heimbachae ATCC 35613, total 6 non-homologous pathogenic proteins were separated....

Background/objectives The primary healthcare system plays an important role in addressing the health needs of communities and preventing diseases. Saudi Arabia is undergoing a major transformation under Arabia's Vision 2030, with main aim increasing efficiency accessibility services to all members community. This reform provides opportunity revolutionize patient care by integrating genetic services. study assesses readiness physicians provide services, identifies possible impacts barriers...

Abstract Background: Tinnitus is a common audiological complaint with multifaceted etiology. Understanding its prevalence and contributing factors crucial for effective management. However, the overall characteristics of tinnitus in Saudi population remain largely unknown. Objectives: This study aimed to determine frequency tinnitus, explore related demographics, assess potential associations between relevant clinical factors, impact on mental health daily activities. Methodology: A...

Sepsis results from a major systemic inflammatory response and can induce disorders in multiple organs. The present study evaluated the potential protective effects of oleuropein (OLE) against hyperinflammatory responses during lipopolysaccharide (LPS)-induced sepsis mice. Sixty male Balb/c mice were randomly categorized into five groups 12 animals each: control, intraperitoneally injected with OLE (50 mg/kg), LPS (10 mg/kg, intraperitoneal), two administered (25 50 mg/kg) for 3 days prior...

Glycogen synthase kinase-3 (GSK3β), a serine/threonine protein kinase, has been discovered as novel target for anticancer drugs. Although GSK3β is involved in multiple pathways linked to the etiology of various cancers, no specific inhibitor authorized cancer therapy. Most its inhibitors have toxicity effects therefore, there need develop safe and more potent inhibitors. In this study, library 4,222 anti-cancer compounds underwent rigorous computational screening identify potential...

Parthenium hysterophorus L. is a poisonous Asteraceae weed. The phytochemical profile, antioxidant activity, total phenolic contents (TPC), flavonoid (TFC), and cytotoxicity of flower extract were evaluated in this study, the toxic effects assessed rabbits. HPLC-DAD system was used for analysis. hemolytic DPPH assays performed. orally administering crude to rabbits (n = 5) at four different doses (10, 20, 40, 80 mg/kg) ten days on hematological biochemical parameters investigated. contained...

Purpose: Helicobacter pylori is one of the most prevalent human pathogens worldwide. However, outcomes H. infection are markedly variable from asymptomatic mild lesion to malignant transformation. Many factors suggested influence these outcomes, including host immunity and genetic susceptibility. Toll-like receptors (TLRs) can recognise different microbial components play an essential role in mucosal immune response against infection. Materials Methods: The association between common single...

Background and objective Osteoarthritis (OA) is influenced by genetics environmental factors, including vitamin D deficiency. This study aimed to investigate the association between levels, growth/differentiation factor 5 (GDF-5) gene polymorphism, serum GDF-5 in obese females with knee OA (KOA) Saudi Arabia. Methodology The enrolled 60 female patients healthy as controls. Blood samples were collected evaluate T>C (rs143383) polymorphism using polymerase chain reaction-restriction...

Diabetes mellitus is a complex metabolic syndrome that involves dysfunction of spleen and other lymphoid organs. Medicinal plants, including okra (Abelmoschus esculentus (L.) Moench), were used widely for diabetes treatment. Scarce data are available about the potential anti-diabetic effects okra, histopathological alterations in splenic tissues mechanistic pathways underlying this association. The current research investigated pod extract on biochemical parameters expression CD8+ T cells...

Here, we investigated the protective efficacy of protocatechuic acid (PCA) against lipopolysaccharide (LPS)-induced septic lung injury. Eighty-two male Balb/c mice were divided into six groups: control, PCA30 (30 mg/kg), LPS (10 PCA10-LPS, PCA20-LPS, and PCA30-LPS treated with 10, 20 30 mg/kg PCA, respectively, for seven days before intraperitoneal injection. PCA pre-treatment, especially at higher dose, significantly reduced LPS-induced tissue injury as indicated by increased heat shock...

Gastric cancer is the fifth most frequent and third major cause of mortality worldwide. Helicobacter pylori, a bacterial infection linked with GC, injects cytotoxin-associated antigen A (CagA; an oncoprotein) into host cells. When phosphorylated CagA protein enters cell, it attaches to other cellular components, interfering normal signaling pathways. plays important role in progression GC by interacting phosphatidylserine cell membrane. Therefore, disrupting CagA-phosphatidylserine...

Background/Objectives: Laryngeal squamous cell carcinoma (LSCC) is the most prevalent form, accounting for nearly 95% of laryngeal cancer. This study evaluates effectiveness chemotherapy and radiation therapy combined treating LSCC, along with exploring miR-449a as a potential biomarker predicting treatment response prognosis in LSCC patients. Patients Methods: The had 81 patients advanced cancer 50 healthy controls. Chemotherapy was given two cycles, followed by an evaluation response....

Osteoporosis (OP) stands as a prevalent bone ailment affecting the elderly, globally. The identification of reliable diagnostic markers crucially aids OP clinical management.

Spleen tyrosine kinase (SYK) is a non-receptor that plays an essential role in signal transduction across different cell types. In the context of allergy and autoimmune disorders, it crucial regulator immune receptor signaling inflammatory cells such as B cells, mast macrophages, neutrophils. Developing SYK inhibitors has gained significant interest for potential therapeutic applications neurological cancer-related conditions. The clinical use most advanced inhibitor, Fostamatinib, been...

Background Diabetes mellitus (DM) is a chronic metabolic disorder. Hepatopathy one of the serious effects DM Melatonin (MT) potent endogenous antioxidant that can control insulin output. However, little information available about potential association between melatonin and hepatic alpha-fetoprotein expression in diabetes. Objective This study was conducted to assess influence MT on diabetes-related injuries determine how β-cells pancreas diabetic rats respond administration. Materials...

Cathepsin L (CTSL) expression is dysregulated in a variety of cancers. Extensive empirical evidence indicates their direct participation cancer growth, angiogenic processes, metastatic dissemination, and the development treatment resistance. Currently, no natural CTSL inhibitors are approved for clinical use. Consequently, novel inhibition strategies an urgent necessity. In this study, combined machine learning (ML) structure-based virtual screening strategy was employed to identify...

In the past decade, use of marine mussels as seafood is being more popular. They considered a rich source various nutritional bioactive compounds that aroused scientific community's interest. This study investigated antioxidant and antithrombotic consequences on Sprague-Dawley male rats after adding dried New Zealand mussel Perna canaliculus in their diet. The biochemical, hematological histopathological changes were also observed. Forty divided into four groups according to amount diet,...