A5101645817- Virus-based gene therapy research
- Mast cells and histamine
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Interference and Gene Delivery
- Systemic Sclerosis and Related Diseases
- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Sarcoma Diagnosis and Treatment
- Dermatologic Treatments and Research
- Skin and Cellular Biology Research
- Neurofibromatosis and Schwannoma Cases
- Eosinophilic Esophagitis
- Gastrointestinal Tumor Research and Treatment
- Eosinophilic Disorders and Syndromes
- Mosquito-borne diseases and control
- Fibroblast Growth Factor Research
- Cellular Mechanics and Interactions
- Peripheral Nerve Disorders
- Virology and Viral Diseases
- Congenital heart defects research
- Vascular Tumors and Angiosarcomas
- Monoclonal and Polyclonal Antibodies Research
- Cerebrospinal fluid and hydrocephalus
- Connective tissue disorders research
- Cardiac tumors and thrombi
Christian Medical College & Hospital
2005-2022
Manipal-Tata Medical College, Jamshedpur
2022
Christian Medical College
2005-2015
Massachusetts General Hospital
2013
Efficient generation of competent vasculogenic cells is a critical challenge human induced pluripotent stem (hiPS) cell-based regenerative medicine. Biologically relevant systems to assess functionality the engineered vessels in vivo are equally important for such development. Here, we report unique approach derivation endothelial precursor from hiPS using triple combination selection markers—CD34, neuropilin 1, and kinase insert domain-containing receptor—and an efficient 2D culture system...
We hypothesized that the AAV2 vector is targeted for destruction in cytoplasm by host cellular kinase/ubiquitination/proteasomal machinery and modification of their targets on capsid may improve its transduction efficiency. In vitro analysis with pharmacological inhibitors serine/threonine kinases (protein kinase A, protein C, casein II) showed an increase (20-90%) AAV2-mediated gene expression. The three-dimensional structure was then analyzed to predict sites ubiquitination...
Abstract Fibrosis is a pervasive disease in which the excessive deposition of extracellular matrix (ECM) compromises tissue function. Although underlying mechanisms are mostly unknown, stiffness increasingly appreciated as contributor to fibrosis rather than merely manifestation disease. Here we show that loss Fibulin-5, an elastic fibre component, not only decreases stiffness, but also diminishes inflammatory response and abrogates fibrotic phenotype mouse model cutaneous fibrosis....
Fibrosis is a prevalent pathological condition arising from the chronic activation of fibroblasts. This results extensive intercellular crosstalk mediated by both soluble factors and direct cell-cell connections. Prominent among these are interactions fibroblasts with immune cells, in which fibroblast-mast cell connection, although acknowledged, relatively unexplored. We have used Tg mouse model skin fibrosis, based on expression transcription factor Snail epidermis, to probe mechanisms...
Recombinant adeno-associated virus vectors based on serotype 8 (AAV8) have shown significant promise for liver-directed gene therapy. However, to overcome the vector dose dependent immunotoxicity seen with AAV8 vectors, it is important develop better that provide enhanced expression at significantly low doses. Since known AAV during intracellular trafficking are targeted destruction in cytoplasm by host-cellular kinase/ubiquitination/proteasomal machinery, we modified specific...
Despite significant advancements with recombinant AAV2 or AAV8 vectors for liver directed gene therapy in humans, it is well-recognized that host and vector-related immune challenges need to be overcome long-term transfer. To these limitations, alternate AAV serotypes (1–10) are being rigorously evaluated. AAV5 the most divergent (55% similarity vs. other serotypes) like AAV1 vector known transduce efficiently. also immunologically distinct by virtue of their low seroprevalence minimal cross...
Systemic sclerosis is a fibrotic disease that initiates in the skin and progresses to internal organs, leading poor prognosis. Unraveling etiology of chronic, multifactorial such as systemic has been aided by various animal models recapitulate certain aspects human pathology. We found transcription factor SNAI1 overexpressed epidermis patients with sclerosis, transgenic mouse recapitulating this expression pattern sufficient induce many clinical features disease. Using model discovery...
Mast cell disease (MCD) is a clonal disorder of the mast and its precursor cells. Cardiac surgery in MCD rarely described. We report an unusual case 14-year-old girl who was admitted for atrial septal defect closure incidentally found to have hepatosplenomegaly with lymphadenopathy underwent successful open-heart surgery, after diagnosis MCD.
Key Clinical Message Ichthyosis prematurity syndrome (IPS) is reported mainly from Scandinavia where most of the cases are homozygous or compound heterozygous for nonsense mutation c.504C>A (p.Cys168*) in exon3 indicating a common ancestor this mutation. The occurrence IPS an Indian patient suggests that it more widespread than previously reported.
Adeno-associated virus (AAV)-mediated gene therapy holds great promise if challenges related to vector neutralization by pre-existing antibodies are circumvented. The use of autologous or allogeneic cells shield the might offer possibility successful transfer in such a situation. In present study, we evaluated feasibility AAV-transduced mesenchymal stromal (MSCs) as vehicle for hepatic murine liver injury model. our initial studies determine most suitable vector, observed that AAV1 (91%) and...
ABSTRACT Fibrosis is a result of chronically activated fibroblasts leading to the overproduction extracellular matrix (ECM), causing tissue hardening and loss organ function. Systemic sclerosis (SSc) fibrotic skin disease marked by inflammation, autoimmunity vasculopathy along with progressive fibrosis internal organs. A major bottleneck in understanding etiology SSc has been lack holistic animal model that can mimic human disease. We found transcription factor Snail overexpressed epidermis...
Severe combined immunodeficient (SCID) mice lack functional T and B cells. This renders them useful for implantation of human The absence immune cells, however, makes severe highly susceptible to infections spontaneous development malignancies; 2 114 CB17/Icr-Prkdc(scid) /IcrIcoCrl aged 9 10 months developed acute leukaemia thymic lymphoma. differential diagnosis such an atypical lymphoid infiltrate includes 'leaky' mice, lymphoma leukaemia. Until this time, the link between neoplasms in...
Malignant triton tumors (MTTs) are peripheral nerve sheath tumors: Perivascular origin is extremely rare. We report a sporadic perivascular MTT in 37-year-old man. He presented with swelling the right popliteal fossa. Imaging revealed solid lesion adherent to artery. underwent en bloc excision of tumor repair studied histopathology and electron microscopy (EM) features this tumor. received local radiotherapy remains disease-free at 2 years. EM may help prognosticate behavior when these occur...