ABSTRACT Analogues based on the insect cecropin–bee melittin hybrid peptide (CEME) were studied and analyzed for activity salt resistance. The new variants designed to have an increase in amphipathic α-helical content (CP29 CP26) overall positive charge (CP26). α-helicity of these peptides was demonstrated by circular dichroism spectroscopy presence liposomes. CP29 shown against gram-negative bacteria that similar or better than those parent peptides, CP26 had activity. cytoplasmic membrane...

A series of alpha-helical cationic antimicrobial peptide variants with small amino acid changes was designed. Alterations in the charge, hydrophobicity, or length variant peptides did not improve activity, and there no statistically significant correlation between any these factors MIC for Pseudomonas aeruginosa, Escherichia coli, Salmonella typhimurium. Individual demonstrated synergy conventional antibiotics against antibiotic-resistant strains P. aeruginosa. The varied considerably...

The lack of suitable animal models for sarcopenic obesity (SO) limits in-depth research into the disease. Emerging studies have demonstrated that gut dysbiosis is involved in development SO. As importance microbial metabolites starting to unveil, it necessary comprehend specific associated with microbiota and We aimed investigate whether high-fat diet (HFD) causes SO natural aging are linking HFD Young rats received or control 80 weeks, obesity-related metabolic disorders sarcopenia were...

Electrical stimulation (ES) is able to enhance angiogenesis by stimulating fibroblasts. Fibroblast growth factor 2 (FGF2) an independent inducer. The present study aimed evaluate the role of ES-induced FGF2 secretion in affecting during wound healing via mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) signaling pathway. Fibroblasts and human umbilical vein endothelial cells (HUVECs) were exposed ES, HUVECs cocultured with ES-treated fibroblast culture...

Abstract Acute burn-induced coagulopathy (ABIC) occurs after severe burns. However, the incidence, prognostic value, and clinical significance of ABIC an extensive burn remain inconclusive due to wide variances in severity coagulation profile evaluation timings previous studies. This retrospective study explored incidence early phase 129 adult patients with burns (>50% total body surface area [TBSA]) admitted centers two hospitals within 10 hours postburn injury during 2009–2017....

This study was the first to investigate genetic abnormalities and structural dysplasia of anorectal malformations (ARMs) in male rats induced by di(n-butyl) phthalate (DBP). DBP administered timed-pregnant establish ARM rat model. The incidence ARMs offspring 39.5%. In neonatal period, decreased body weight anogenital distance were observed. general image histological analysis confirmed presence ARMs. Anatomical examination revealed solid organs (heart-lung, liver, spleen, kidney). decreases...

Abstract Background Recurrent implantation failure (RIF) brings great challenges to clinicians and causes deep frustration patients. Previous data has suggested that prednisone may play a promising role in the establishment of pregnancy help improve outcome women with RIF. But there is insufficient evidence from randomized clinical trials had adequate power determine if can enhance live births as primary outcome. Methods/design This trial prospective, multicenter, randomized, double-blind,...

Wound healing refers to the process of skin and other tissues appear be broken or defective after one body suffers external force. Accelerating wound through use natural products isolated from plants microbes is a hot research topic. Tetrahydrocurcumin major in vivo colorless hydrogenated metabolites curcumin with superior anti-inflammatory properties. The poor bioavailability these curcuminoids limits their clinical applications. Preparation curcuminoids-loaded nanoparticles appropriate...

Abstract Zinc finger gene 217 (ZNF217) is a candidate of polycystic ovary syndrome (PCOS) which vulnerable to ovarian hyperstimulation (OHSS). However, the relationship between ZNF217 and OHSS largely unknown. Our study demonstrated that was mainly distributed in granulosa cells rat ovary. Significantly higher expression detected rats, being consistent with serum 17β-estradiol concentration aromatase. Moreover, rats also showed decreased TSP-1 mRNA, an acknowledged VEGF signaling suppressor....

Polycystic ovary syndrome, a common condition characterized by endocrine dysfunction, menstrual irregularity, anovulation and polycystic ovaries, affects 5–7% of reproductive-age women. RAB5B , which is identified genome-wide association study as risk locus for this encodes small GTPase involved in control receptor internalization early endosome fusion. We found that RAB5A mRNA levels luteinized granulosa cells obese patients with syndrome were lower than those women without the syndrome....

Several studies have suggested that migraineurs suffer from neurocognitive abnormalities, but this phenomenon and exact mechanisms remain controversial. In study, we aimed to reevaluate visual spatial attention via event-related potential (ERP) examinations explore further correlations between ERP data migraine characteristics.Altogether, 25 patients (9 males, 16 females; mean age 35.240 years) in the interictal period 21 age-matched healthy controls (8 13 35.286 were recruited. A modified...

Patch-clamp methods were used to examine the effects of volatile general anesthetic halothane on large-conductance calcium-dependent potassium channels (BK channels) in dispersed cerebrovascular smooth muscle cells adult rats.Inside-out membrane patches for recordings at 21-23 degrees C. Halothane was administered aqueous concentrations 0.5-2.8 mM conjunction with free cytoplasmic calcium 1 or 100 microM and a potential -60 mV +60 mV.At concentration microM, clinically relevant dose 0.5 (2...

Objective: To analyze the relationship between serum lactic acid value and risk of death in patients with extensive burn during shock stage related influencing factors. Methods: Clinical data 127 (111 males 16 females) admitted to Institute Burn Research First Affiliated Hospital Army Medical University from January 2009 December 2013 Department Plastic Surgery Burns Southwest 2016 2018, who met admission criteria, were retrospectively analyzed. The aged 21 62 years, total area more than 50%...

Background and Objectives Chronic wound repair is a major problem in treatment. Recently, several studies have suggested that carbon dioxide (CO 2 ) laser can be used to improve the healing of chronic wounds. The aim present study was preliminarily investigate efficacy debridement treating through comparison traditional instrument/surgical with ultrapulsed CO terms healing, infection control, blood perfusion. Study Design/Materials Methods Patients admitted Wound Repair Clinic at Affiliated...

This study used laser speckle contrast imaging (LSCI) to evaluate the difference in blood perfusion between hypertrophic scars and keloids.A total of 30 keloids, 21 early scars, 20 proliferative regressive mature were enrolled into this study. Vancouver Scar Scale (VSS) was assessed by a plastic surgeon. LSCI whole (W), marginal (M), central (C) regions, surrounding normal skin ratios (M/N, C/N) calculated.The region keloid significantly higher than that region. Nevertheless, there no...

Several reports have indicated potential cognitive decline for cerebral small vessel disease (CSVD), especially in attention domain, whereas the attentional function at network level is still elusive. In this study, we used test (ANT) paradigm to characterize efficiency of alerting, orienting, and executive control networks patients with CSVD explore possible correlations between efficiencies obtained total score.A 31 30 healthy controls matched age, gender, education were recruited. After...

Purpose: To compare the clinical result of mini-dose GnRH-a long protocol with short in older patients undergoing IVF.Materials and methods: This was a retrospective study. Four hundred sixty-one women aged above 35-year-old first cycle were assigned to two groups: (n = 359); 102). Both groups divided based on their age, into over under 38 years old. Primary outcome include live birth rate per started cycle. Other outcomes good-quality embryo rate, pregnancy rate.Results: Patients treated...