A5100716267- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Protist diversity and phylogeny
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Bacteriophages and microbial interactions
- Microbial Natural Products and Biosynthesis
- Cancer-related gene regulation
- Aquaculture disease management and microbiota
- Immunotherapy and Immune Responses
- Plant Virus Research Studies
- DNA and Nucleic Acid Chemistry
- Vibrio bacteria research studies
- DNA Repair Mechanisms
- Medicinal Plants and Bioactive Compounds
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Cancer Genomics and Diagnostics
- Plant Pathogens and Fungal Diseases
- Protein Hydrolysis and Bioactive Peptides
- RNA Research and Splicing
- Genetic factors in colorectal cancer
Chinese Academy of Medical Sciences & Peking Union Medical College
2021-2025
Yunnan University
2014-2024
Shantou University
2024
Zhengzhou University
2017-2023
Chinese University of Hong Kong
2018-2022
Qingdao University
2006-2021
Qingdao Municipal Hospital
2021
State Key Laboratory of Plant Genomics
2017-2020
Institute of Genetics and Developmental Biology
2017-2020
Chinese Academy of Sciences
2017-2020
Abstract: DNA nanostructures prepared by self-assembly possess good stability, high biocompatibility, and low immunogenicity as drug delivery vehicles. In this work, tetrahedron (TD) was constructed modified with SL2B aptamer (S) folic acid (F). TD possessed a small diameter (~6 nm) entered into the nucleus quickly. can inhibit cancer cell growth disturbing vascular endothelial factor/Notch signaling pathways. To explore effect of number on colorectal inhibition, multimers (dimer, trimer,...
Craniofacial microsomia (CFM) is a congenital defect that usually results from aberrant development of embryonic pharyngeal arches. However, the molecular basis CFM pathogenesis largely unknown. Here, we employ zebrafish model to investigate mechanisms pathogenesis. In early embryos, tet2 and tet3 are essential for cartilage development. Single-cell RNA sequencing reveals loss Tet2/3 impairs chondrocyte differentiation due insufficient BMP signaling. Moreover, biochemical genetic evidence...
Rosmarinic acid (RA) has numerous pharmacologic effects, including anti-oxidant, anti-inflammatory, and analgesic effects. This study aimed to evaluate the preventive activity of RA in a murine model asthma investigate its possible molecular mechanisms. Female BALB/c mice sensitized challenged with ovalbumin (Ova) were pretreated (5, 10 or 20 mg/kg) at 1 h before Ova challenge. The results demonstrated that markedly inhibited increases inflammatory cells Th2 cytokines bronchoalveolar lavage...
Salidroside is a major component extracted from Rhodiola rosea. In this study, we investigated protective effects of salidroside on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. the mouse model, found that pretreatment with single 120 mg/kg dose prior to administration intratracheal LPS induced significant decrease W/D ratio and myeloperoxidase activity lung, reduction protein concentration, number total cells, neutrophils macrophages bronchoalveolar lavage fluid....
Summary The taxonomy of marine and non‐marine organisms rarely overlap, but the mechanisms underlying this distinction are often unknown. Here, we predicted three major ocean‐to‐land transitions in evolutionary history Flavobacteriaceae , a family known for polysaccharide peptide degradation. These unidirectional were associated with repeated losses signature genes gains adaptive genes. This included various Na + ‐dependent transporters, osmolyte transporters glycoside hydrolases (GH)...
Many major marine bacterial lineages such as SAR11, Prochlorococcus, SAR116, and several Roseobacter have members that are abundant, relatively slow-growing, genome streamlined. The isolation of phages infect SAR11 SAR116 demonstrated the dominance these in virosphere. However, no been isolated from bacteria RCA lineage, another abundant group bacteria. In this study, seven three different strains were characterized. All belong to Podoviridae family sizes ranging 39.6 58.1 kb. Interestingly,...
Viruses play a key role in biogeochemical cycling and host mortality, metabolism, physiology evolution the ocean. that infect globally abundant SAR11 bacteria (pelagiphages) were reported to be an important component of marine viral communities. Our current knowledge pelagiphages is based on few studies therefore limited. In this study, 10 new isolated genomically characterized. These represent first cultivated representatives four lineages only found metagenomic sequencing datasets...
A Gram-stain-positive, non-motile, rod-shaped, catalase-positive and oxidase-negative bacterium, designated YIM CS25T, was isolated from a soil sample collected Turpan desert in Xinjiang Uyghur Autonomous Region, north-western China. The isolate grew at 15-40 °C, pH 6.0-8.0 with 0-6 % (w/v) NaCl. phylogenetic trees based on 16S rRNA gene sequences revealed that strain CS25T belonged to the genus Arthrobacter closely related halodurans JSM 078085T (95.89 similarity). peptidoglycan type...
ABSTRACT The metabolism and reproduction of plants depend on the division labors among cells. However, cells with various functions are often studied as a bulk where their specificities were diluted. Here, we apply single-cell RNA sequencing to aerial part rice seedlings growing in environments. We capture transcriptomes thousands cells, identify all major cell types, reconstruct developmental trajectories. find that abiotic stresses not only affect gene expression cell-type-specific manner...
Abstract Background Liver cancer (LC) is one of the most fatal cancers throughout world. More efficient and sensitive gene signatures that could accurately predict survival in LC patients are vitally needed to promote a better individualized effective treatment. Material/methods 422 adjacent normal tissues with both RNA-Seq clinical data TCGA were embedded our study. Gene set enrichment analysis (GSEA) was applied identify genes hallmark sets more valuable for liver therapy. Cox regression...
Background: Aptamers have been widely used as targeted therapeutic agents due to its relatively small physical size, flexible structure, high specificity, and selectivity. functionalized nanomaterials, not only enhance the targeting of but can also improve stability aptamers. We developed aptamer C2NP (Apt) conjugated straight DNA nanotubes (S-DNT-Apt) twisted (T-DNT-Apt) nanocarriers for doxorubicin (DOX). Methods: The (T-DNT) (S-DNT) were assembled with a scaffold hundreds staples. Apt was...
Abstract Background PolyC-RNA-binding protein 1 (PCBP1) functions as a tumour suppressor and RNA regulator that is downregulated in human cancers. Here, we aimed to reveal the biological function of PCBP1 lung adenocarcinoma (LUAD). Methods First, was identified an important biomarker maintains LUAD through The Cancer Genome Atlas (TCGA) project screening confirmed by immunohistochemistry qPCR. Via colony formation, CCK8, IncuCyte cell proliferation, wound healing Transwell assays, closely...
Abstract Marine bacterioplankton communities are dominated by cells equipped with small genomes. Streamlining selection has been accepted as the main force driving their genome reduction. Here, we report that a neutral evolutionary mechanism governs reduction in Roseobacter group represents 5-20% of coastal waters. Using representative strains fall into three size groups (2-3, 3-4, and 4-5 Mbp), measured genomic mutation rates (μ) through long-term accumulation experiments followed...
A Gram-stain-negative, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated YIM X0211T, was isolated from a soil sample of Shiling County, Yunnan Province, south-west China. The new isolate characterized taxonomically by using polyphasic approach. strain grew optimally at 30 °C, pH 7.0 and with 0–3 % (w/v) NaCl. It positive for catalase oxidase but negative H2S production. Comparative 16S rRNA gene sequence analysis showed that X0211T fell within the...
Two extremely halophilic archaea, strains YIM 93745T and 93707, were isolated from a saline soil sample collected Loulan, China. Cells of the two coccus, non-motile Gram-stain negative. The aerobic grew at 25-50 °C (optimum, 37 °C), in presence 5-35 % (w/v) NaCl 20 %), 0.01-0.1 M Mg2+ 0.03 M) pH 6.0-8.5 7.0-7.5). lysed distilled water with 0-5 NaCl. Major polar lipids phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, sulfate, sulfated mannosyl glycosyl diether unidentified...