A5052720019- Acute Myeloid Leukemia Research
- Diabetes Treatment and Management
- Statistical Methods in Clinical Trials
- Melanoma and MAPK Pathways
- T-cell and B-cell Immunology
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Lymphatic System and Diseases
- Pancreatic and Hepatic Oncology Research
- Microscopic Colitis
- Asthma and respiratory diseases
- Gestational Diabetes Research and Management
- Inflammatory Bowel Disease
- Retinoids in leukemia and cellular processes
- HIV/AIDS oral health manifestations
- Peptidase Inhibition and Analysis
- Cytokine Signaling Pathways and Interactions
- HIV Research and Treatment
- Immune Cell Function and Interaction
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Antifungal resistance and susceptibility
- Clostridium difficile and Clostridium perfringens research
- Neuroendocrine Tumor Research Advances
- Immunodeficiency and Autoimmune Disorders
- Optimal Experimental Design Methods
- Animal Virus Infections Studies
University of North Carolina at Chapel Hill
2013-2023
Canadian Nuclear Laboratories
2022
Hôpital Maisonneuve-Rosemont
2007-2019
Université de Montréal
2007-2019
Institute for Research in Immunology and Cancer
2017-2019
The Coordinating Center
2019
Leukemia & Lymphoma Society of Canada
2016
Importance Insulin is recommended for pregnant persons with preexisting type 2 diabetes or diagnosed early in pregnancy. The addition of metformin to insulin may improve neonatal outcomes. Objective To estimate the effect added pregnancy on a composite adverse outcome. Design, Setting, and Participants This randomized clinical trial 17 US centers enrolled adults aged 18 45 years prior 23 weeks’ gestation between April 2019 November 2021. Each participant was treated assigned add either...
To compare pancreatic cancer incidence and diagnostic evaluation among patients initiating dipeptidyl-peptidase-4 (DPP-4) inhibitor treatment with those sulfonylureas (SU) thiazolidinediones (TZD).
Abstract In acute myeloid leukemia (AML), risk stratification based on cytogenetics and mutation profiling is essential but remains insufficient to select the optimal therapy. Accurate biomarkers are needed improve prognostic assessment. We analyzed RNA sequencing survival data of 430 AML patients identified HMGA2 as a novel marker. validated quantitative PCR test study association expression with clinical outcomes in 358 samples. this training cohort, was highly expressed 22.3% AML, mostly...
The ability of IL-21 to promote in vitro T cell survival led us investigate its biological activity vivo. We report that overexpression transgenic mice drives CD8(+) memory accumulation with a concomitant reduction naive numbers. These cells are functional, given their rapidly produce IFN-gamma and proliferate following stimulation. Since the homeostasis is controlled by cytokines, we evaluated whether influences cytokine receptor expression. show inhibits IL-7R expression on vitro,...
Abstract IL21 is a Type I cytokine, which uses the common γ chain (γc) in its receptor. As members of γc cytokine/cytokine receptors family play crucial role differentiation, activation, and survival lymphocytes, we have investigated if IL-21 could promote T cell thus, contribute to homeostasis expansion. Unlike most cytokine receptors, report that IL-21R constitutively expressed by all mature lymphocytes stromal cells lymphoid organs are constitutive source IL-21. These observations...
OBJECTIVE To add to the evidence on comparative long-term effects of insulin analog glargine versus human NPH risk for cancer. RESEARCH DESIGN AND METHODS We identified cohorts initiators and without an prescription during prior 19 months among patients covered by Inovalon Medical Outcomes Research Effectiveness Economics Registry (MORE2 Registry) between January 2003 December 2010. Patients were required have a second same within 180 days be free balanced factors cancer outcomes based...
Candida albicans causes oropharyngeal candidiasis (OPC) but rarely disseminates to deep organs in human immunodeficiency virus (HIV) infection. Here, we used a model of OPC CD4C/HIV(Mut) transgenic (Tg) mice investigate the role polymorphonuclear leukocytes (PMNs) and CD8+ T cells limiting mucosa. Numbers circulating PMNs their oxidative burst were both augmented CD4C/HIV(MutA) Tg expressing rev, env, nef HIV type 1 (HIV-1), while phagocytosis killing C. largely unimpaired compared those...
ABSTRACT We previously reported that CD4C/human immunodeficiency virus (HIV) Nef transgenic (Tg) mice, expressing in CD4 + T cells and of the macrophage/dendritic cell (DC) lineage, develop a severe AIDS-like disease, characterized by depletion cells, as well lung, heart, kidney diseases. In order to determine contribution distinct populations hematopoietic development this five additional Tg strains through restricted cell-specific regulatory elements were generated. These express DCs,...
The classical mitogen-activated protein kinases (MAPKs) ERK1 and ERK2 are activated upon stimulation of cells with a broad range extracellular signals (including antigens) allowing cellular responses to occur. ERK3 is an atypical member the MAPK family highest homology ERK1/2. Therefore, we evaluated role in mature T cell response. Mouse resting do not transcribe but its expression induced both CD4⁺ CD8⁺ following receptor (TCR)-induced activation. This induction lymphocytes requires...
In contrast to pediatric Crohn's disease (CD), little is known in ulcerative colitis (UC) about the relationship between phenotype and serologic reactivity microbial other antigens. The aim of this study was examine serology a well-characterized inception cohort children newly diagnosed with UC during PROTECT Study (Predicting Response Standardized Pediatric Colitis Therapy). Patients were recruited from 29 participating centers. Demographic, clinical, laboratory, (pANCA, ASCA IgA/IgG,...
Extracellular signal-regulated kinase 3 (ERK3) is an atypical member of the mitogen-activated protein (MAPK) family whose function largely unknown. Given central role MAPKs in T cell development, we hypothesized that ERK3 may regulate thymocyte development. Here have shown deficiency leads to a 50% reduction CD4+ CD8+ (DP) number. Analysis hematopoietic chimeras revealed DP thymocytes intrinsic cells. We found early thymic progenitors seed Erk3−/− thymus and can properly differentiate...
Extracellular signal-regulated kinase 3 (ERK3 )is an atypical member of the mitogen-activated protein (MAPK) family. We have previously shown that ERK3 is expressed during thymocyte differentiation and its expression induced in mature peripheral T cells following activation ERK1/2 by T-cell receptor (TCR) signalling. Herein, we investigated whether required for proper selection. Using a knock-in mouse model which coding sequence replaced gene encoding β-galactosidase reporter, show...
The Precision Interventions for Severe and/or Exacerbation-prone Asthma (PrecISE) study is an adaptive platform trial designed to investigate novel interventions severe asthma. conducted under a master protocol and utilizes crossover design with each participant receiving up five at least one placebo. Treatment assignments are based on the patients' biomarker profiles precision health methods incorporated into interim final analyses. We describe key elements of PrecISE including multistage...
To evaluate the relative propensities of oritavancin and vancomycin to induce Clostridium difficile infection (CDI) in hamster vitro human gut models. Hamsters received clindamycin (100 mg/kg orally or subcutaneously), (50 orally) orally). C. spores were administered next day. Control hamsters vehicle only (polyethylene glycol 400) plus but no spores. monitored for clinical signs 20 days. Caecal contents analysed cells, presence (cyto)toxin. Oritavancin instilled over 7 days into separate...
Vaccines containing mRNA with the capacity to self-amplify represent an alternative vaccines that came prominence during COVID-19 pandemic. To gain further insights on safety profile of self-amplifying mRNA- (SAM-) vaccines, this preclinical toxicology study in rats evaluated effect (i) type delivery system (lipid nanoparticle [LNP] vs cationic nano-emulsion [CNE]); (ii) antigen-encoding sequence (rabies glycoprotein G SARS-CoV-2 Spike); and (iii) RNA amplification. Further analyses also...
Abstract The impairments of protective mucosal immunity which cause susceptibility to oropharyngeal candidiasis (OPC) in HIV infection remain undefined. This study used a model OPC CD4C/HIV MutA transgenic (Tg) mice expressing Rev, Env, and Nef HIV-1 investigate the role transgene dendritic cells (DCs) CD4+ T maintenance chronic oral carriage Candida albicans. DCs were depleted Tg had an immature phenotype, with low expression MHC class II IL-12. quantitatively reduced mucosa, cervical lymph...
Current strategies for risk stratification of patients with acute myeloid leukemia assign approximately 40% to the intermediate-risk group, where uncertainty about optimal therapy still persists.The objective this study was assess cost effectiveness a HMGA2 prognostic test based on HMGA2+/HMGA2- expression, which improves genetic in leukemia, and compare current standard care Canada.A cost-effectiveness model developed from Canadian National Healthcare Service societal perspective using data...
(Abstracted from JAMA 2023;330(22):2182–2190) Preexisting type 2 diabetes in pregnant people increases the risk for adverse pregnancy outcomes, intrapartum complications, and neonatal hypoglycemia at birth. To achieve normal maternal glucose levels optimize management includes blood monitoring, dietary management, insulin therapy.