A5106691619- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Streptococcal Infections and Treatments
- Influenza Virus Research Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Bacterial Infections and Vaccines
- Gastrointestinal disorders and treatments
- Circular RNAs in diseases
- Viral gastroenteritis research and epidemiology
- COVID-19 diagnosis using AI
- Mobile Health and mHealth Applications
- Congenital Anomalies and Fetal Surgery
- Problem and Project Based Learning
- Immunodeficiency and Autoimmune Disorders
- Biomedical and Engineering Education
- SARS-CoV-2 detection and testing
- Intravenous Infusion Technology and Safety
- COVID-19 Digital Contact Tracing
- Infection Control and Ventilation
- Cancer-related molecular mechanisms research
- Antimicrobial Resistance in Staphylococcus
- RNA Research and Splicing
- Child Nutrition and Water Access
- SARS-CoV-2 and COVID-19 Research
- Fecal contamination and water quality
Aga Khan University
2017-2025
Donghua University
2021
Tianjin University
2021
Wuhan University of Technology
2021
Higher Institute of Engineering
2021
Shaheed Zulfiqar Ali Bhutto Institute of Science and Technology
2021
This study aimed to identify differentially expressed non-coding RNAs (ncRNAs) associated with preterm birth (PTB) and determine biological pathways being influenced in the context of PTB. We processed cell-free RNA sequencing data identified seventeen (DE) ncRNAs that could be involved onset Per validation via customized RT-qPCR, recorded variations expressions eleven were concordant in-silico analyses. The results this provide insights into role DE their impact on pregnancy-related lead...
Introduction Environmental enteropathy is an important contributor to childhood malnutrition in the developing world. Chronic exposure fecal pathogens leads alteration intestinal structure and function, resulting impaired gut immune malabsorption, growth faltering leading environmental enteropathy. Methods A community-based intervention study was carried out on children till 24 months of age Matiari district, Pakistan. Blood specimens were collected from enrolled aged 3–6 9 months. real-time...
The etiology of intussusception, the leading cause bowel obstruction in infants, is unknown most cases. Adenovirus has been associated with intussusception and slightly increased risk rotavirus vaccination found. We conducted a case-control study among children <2 years old Bangladesh, Nepal, Pakistan, Vietnam to evaluate infectious etiologies before vaccine introduction.
Pakistan was one of the first South-Asian countries to introduce ten-valent pneumococcal conjugate vaccine (PCV10) at national level, using a 3+0 schedule without catchup, in 2013.From 2014-18, fifteen children <2 years old were recruited every week Matiari, Sindh, and nasopharyngeal swabs collected. The samples cultured, pneumococcus further serotyped through multiplex PCR Aga Khan University Hospital as per method described by Centers for Disease Control Prevention, USA.Pneumococcus...
Background: Pakistan’s immunization uptake rates are still significantly lower than anticipated despite several initiatives. Lack of awareness, forgetting about vaccination schedule, and vaccine misconception/misinformation a few the major drivers that mitigate immunization. The current COVID-19 pandemic emphasizes importance significant reductions in regular childhood during have increased risk outbreaks vaccine-preventable diseases. Concerns among parents over possibly exposing their...
Benefits of pneumococcal conjugate vaccine programs have been linked to the vaccine's ability disrupt nasopharyngeal carriage and transmission. The 10-valent (PCV10) was included in Expanded Program on Immunization (EPI) Sindh, Pakistan February 2013. This study carried out immediately before PCV10 introduction establish baseline prevalent serotypes young children determine if differed urban rural communities. Nasopharyngeal specimens were collected from a random sample 3-11 12-59 months age...
Laboratory biosafety is currently a major global issue in clinical research and academic laboratories. To alleviate these concerns, subject-specific education of standardized laboratory practices essential should be delivered comprehensible, interactive, appealing manner. The objective the present study was to engage university-level students biosecurity related through Socratic method learning. A pre/post-test model implemented assess knowledge improvement after workshop. Two workshops were...
Next-generation sequencing technology has revolutionised pathogen surveillance over the last two decades. However, benefits are not equitably distributed, with developing countries lagging far behind in acquiring required and analytical capacity. Recent declines cost associated sequencing—equipment running consumables have created an opportunity for broader adoption. During COVID-19 pandemic, rapid diagnostics development DNA ability to diagnose sequence SARS-CoV-2 rapidly. Socioeconomic...
Pneumonia is the leading cause of morbidity and mortality in children worldwide. The ten valent pneumococcal vaccine (PCV10) was introduced Pakistan's Expanded Program on Immunization (EPI) 2012 as a 3 + 0 schedule without catchup immunization. Nasopharyngeal carriage taken surrogate marker to measure impact populations. Carriage surveys are necessary monitor persistence Vaccine Type (VT) serotypes, emergence Non-Vaccine (NVT) their role both transmission disease. This article describes...
The 10-valent pneumococcal vaccine was introduced in Pakistan’s Expanded Program on Immunization (EPI) 2013 as a 3 + 0 schedule without catchup. We conducted three annual cross-sectional surveys from 2014−2016 to measure vaccine-type (VT) carriage infants rural part of Pakistan. Nasopharyngeal specimens were collected by random sampling two union councils Matiari. Samples then transported the Infectious Disease Research Laboratory (IDRL) at Aga Khan University within 6−8 h collection....
Staphylococcus aureus and Streptococcus pneumoniae are common colonizers of the human nasopharynx. In this study, we describe S. nasopharyngeal carriage evaluate its association with post-10-valent pneumococcal conjugate vaccine (PCV10) introduction in Pakistan.A serial cross-sectional study was undertaken from 2014 to 2018, children <2 years were randomly selected, swabs collected using standard WHO guidelines. isolates identified methods tested for antimicrobial susceptibility by...
In early 2021, the 10-valent Pneumococcal conjugate vaccine (PCV10) was replaced with 13-valent (PCV13) by federal directorate of immunization (FDI), Pakistan. We assessed impact a higher valent vaccine, PCV13, on serotype distribution nasopharyngeal carriage in rural
The 10-valent pneumococcal vaccine was introduced in Pakistan&rsquo;s Expanded Program on Immunization (EPI) 2013 as a 3+0 schedule without catchup. We conducted three annual cross-sectional surveys from 2014-2016 to measure vaccine-type (VT) carriage infants rural part of Pakistan. Nasopharyngeal specimens were collected by random sampling 2 union councils Matiari. Samples then transported Infectious Disease Research Laboratory (IDRL) at the Aga Khan University within 6-8 hours...
Background: We present our experience with optimization and diagnostic use of quantitative real-time polymerase chain reaction (PCR) targeting the lytA gene Streptococcus pneumoniae for detection S. in whole blood children <5 years age. The assay was optimized to detect ≥5 CFU/10 μl or 1 copy DNA/2 blood. Methods: This applied on 1912 specimens collected from age pneumonia, which 35 were positive. bacterial loads determined through categorization load into five different categories, i.e.,...